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|Cell Counting Kit-8
|Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.
|Y-27632 is a potent, selective inhibitor of Rho-associated protein kinases (ROCK) with IC50 values of 140-220 nM for ROCK1 and ROCK2. In addition, Y-27632 inhibited LPA-induced entosis.
|Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
|Protease inhibitor cocktails are used in mammalian cell lysates or tissue extracts to increase protein stability.
|LY294002 is a highly selective PI3K inhibitor with IC50 values of 0.31, 0.73, 1.06 and 6.60 μM for PI3Kβ, PI3Kα, PI3Kδ and PI3Kγ respectively. LY294002 also inhibits CK2 with an IC50 of 98 nM. LY294002 is a competitive DNA-PK inhibitor that binds reversibly to the kinase domain of DNA-PK with an IC50 of 1.4 μM.
|Dimethyl sulfoxide (cell cryopresevation)
|Dimethyl sulfoxide (abbreviated DMSO) is a common organic solvent which has the strongest dissolving ability, it can dissolve most of the organic compounds.
|MG132 is a specific and potent cell-permeable proteasome inhibitor (IC50 = 100 nM, Ki = 4 nM).
|Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, with IC50 of 532.5 nM.
|Actinomycin d is a bright red rhomboid prisms or red powder.
|Phosphatase Inhibitor Cocktail (EDTA-Free)
|AbMole Phosphatase Inhibitor Cocktail (EDTA-Free) is a mixture of phosphatase inhibitors prepared by 8 independent phosphatase inhibitors according to the optimized ratio. It is divided into A and B two tubes: A tube is a phosphate - root analogue to prevent the loss of phosphorylation of proteins and lipids, and B tube is a new natural product to provide more comprehensive protection for phosphorylation.
|Triton X-100 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.
|Rapamycin (Sirolimus,Rapamune)is an inhibitor of mTOR.The mammalian target of rapamycin (mTOR) is a kinase responsible for mitogen-induced cell proliferation/survival signaling.
|MK-2206 is a novel allosteric inhibitor of Akt with IC50 values of 8 nM, 12 nM and 65 nM for Akt1, Akt2 and Akt3, respectively.
|Acetylcysteine (NAC; N-acetyl-l-cysteine) is a ROS inhibitor that antagonizes the activity of several proteasome inhibitors. It is also an inhibitor of the tumor necrosis factor TNF.
|ML385 was a specific nuclear factor erythroid 2-related factor 2 (NRF2) inhibitor with an IC50 of 1.9 μM. ML385 inhibits Nrf2 transcription factor activity by binding to Neh1.
|Streptozotocin (STZ) is a kind of glucosamine - nitrosourea derivative. *The compound is unstable in solutions, freshly prepared is recommended
|3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM, respectively.
|HEPES is a multipurpose buffer.
|Dorsomorphin (Compound C) dihydrochloride
|Dorsomorphin (BML-275) dihydrochloride is a potent inhibitor of AMP-activated protein kinase (AMPK), with a Ki of 109 nM. Dorsomorphin dihydrochloride inhibits BMP pathway by targeting the type I receptors ALK2, ALK3, and ALK6. Dorsomorphin dihydrochloride induces autophagy.
|Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor which blocks all features of apoptosis.
|H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm). H2DCFDA can be used for the Bacterial reactive oxygen species (ROS) production assay.
|Phorbol 12-myristate 13-acetate
|Phorbol 12-myristate 13-acetate (PMA) is a dual SphK and protein kinase C (PKC) activator, increases the intracellular Ca2+ concentration ([Ca2+]i) in a dose-dependent manner, with an EC50 of 11.7 nM.
|U0126-EtOH is a potent and highly selective non-competitive inhibitor of MAPK/ERK kinase with IC50 of 72 nM for MEK1 and 58 nM for MEK2.
|PMSF is an irreversible serine/cysteine protease inhibitor.
|Cisplatin is an inorganic platinum complex that inhibits DNA synthesis by forming DNA crosslinking agents, inactivates GPXs, reduces cell GSH and induces iron death in HCT116 and A549 cells.
|Streptomycin sulfate is an antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.
|Talazoparib (BMN-673) is a highly potent, orally active PARP1/2 inhibitor.Talazoparib inhibits PARP1 and PARP2 enzyme activity with Kis of 1.2 nM and 0.87 nM, respectively. Talazoparib has antitumor activity.
|Pyrrolidinedithiocarbamate ammonium (PDTC)
|Pyrrolidinedithiocarbamate ammonium (PDTC) is a selective nuclear factor-κB (NF-κB) inhibitor.
|MCC950 (CP-456773) is a potent, selective NLRP3 inhibitor with IC50 of 7.5 nM and 8.1 nM in BMDMs and HMDMs, respectively.
|DAPI dihydrochloride is A fluorescent dye that binds DNA rich in a-T sequences. The maximum excitation/emission wavelength is 358nm/461 nm, and the blue fluorescence is often used to observe the nucleus and nucleic acid. Store at room temperature away from light.
|Adezmapimod (SB203580) is a P38 MAPK inhibitor with an IC50 of 0.3-0.5 μM, which is 10 times less selective than SAPK3(106T) and SAPK4(106T), and inhibits PKB phosphorylation with an IC50 of 3-5 μM.
|MCC950 sodium salt (CP-456773)
|MCC950 (CP-456773) sodium salt is a potent, selective NLRP3 inhibitor with IC50s of 7.5 and 8.1 nM in BMDMs and HMDMs, respectively. MCC950 specifically inhibits NLRP3 but not AIM2, NLRC4 or NLRP1 activation.
|Penicillin G Sodium
|Penicillin G Sodium is a β-lactam antibiotic produced by Penicillin spp.
|Lipopolysaccharides (LPS) is an endotoxin derived from the outer leaflet of the outer membrane of Gram-negative bacteria. This product is derived from Escherichia coli O55:B5.
|SP600125 is a novel and selective inhibitor of c-Jun N-terminal kinase (JNK), inhibited phosphorylation of c-Jun with an IC50 of 5-10 microM.
|Temozolomide (TMZ) is an oral DNA methylating, chemotherapeutic agent.
|Resatorvid (TAK-242) is a selective TLR4 signaling inhibitor. Resatorvid inhibited the production of NO, TNF-α and IL-6 with IC50 values of 1.8 nM, 1.9 nM and 1.3 nM, respectively. Resatorvid down-regulates the expression of MyD88 and TRIF signaling molecules downstream of TLR4. Resatorvid inhibits autophagy and plays a key role in a variety of inflammatory diseases.
|SCH772984 is a novel and selective inhibitor of ERK1/2 with IC50 of 4 nM and 1 nM, respectively.
|Tofacitinib (formerly tasocitinib, CP-690550) is a novel Janus Kinase 3 (JAK-3) kinase inhibitor with an IC50 value estimated at 0.2 μM.
|XAV939 is a potent, cell-permeable small molecule inhibitor of the Wnt/β-catenin pathway to TNKS1 and TNKS2 with IC50 values of 11 and 4 nM respectively.
|Mirdametinib (PD0325901) is a highly potent and selective MEK inhibitor with an IC50 in C26 cells of 0.33nM.
|Ferrostatin-1 (Fer-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.
|Puromycin dihydrochloride is an aminonucleoside antibiotic that acts as a protein synthesis inhibitor.
|Fingolimod (FTY720) HCl is a 1-phospho-neuraminol S1P receptor antagonist with an IC50 of 0.33 nM. , FTY720 can dephosphorylate AMPK by activating PP2A and reduce the expression level of phosphorylated eEF2K, ultimately leading to ADCD and iron death in human multiple myeloma cells.
|AZD 2281 (Olaparib, KU-0059436) is a potent PARP (poly ADP-ribose polymerase) inhibitorwith IC50 of 5 and 1 nM for PARP-1and PARP-2 respectively. Olaparib is an autophagy and mitophagy activator.
|Deferoxamine mesylate as a chelating agent used to remove excess iron from the body.
|CHIR-99021 (CT99021) is an aminopyrimidine derivative that inhibits GSK3α and GSK3β with IC50 values of 10 and 6.7 nM, respectively.
|Ionomycin is a calcium ionophor and an antibiotic produced by Streptomyces conglobatus.
|Rosiglitazone (BRL 49653) is an orally active selective PPARγ agonist (EC50=60 nM, Kd=40 nM). Rosiglitazone is an TRPC5 activator (EC50=30 μM) and TRPM3 inhibitor.
|Chloroquine is an inhibitor of autophagy and Toll-like receptors (TLRs). Chloroquine can effectively inhibit SARS-COV-2 infection (EC50=1.13 μM). Chloroquine is an anti-inflammatory widely used in malaria and rheumatoid arthritis.
|Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS. *The compound is unstable in solutions, freshly prepared is recommended
|DAPT (GSI-IX) is a γ-secretase inhibitor with IC50 values of 115 and 200 nM for total Aβ and Aβ42 respectively.
|AG490 (Tyrphostin AG490) is a selective inhibitor of EGF receptor tyrosine kinase (IC50 values are 2 and 13.5 μM for EGFR and ErbB2 respectively).
|Paclitaxel is a diterpenoid derived from the bark of the plant Picea abies, and is also a microtubule polymer stabilizer, which promotes polymerization of microtubule proteins to inhibit depolymerization, maintains the stability of microtubule proteins, and inhibits cellular mitosis, and acts on human endothelial cells with an IC50 of 0.1 pM.
|Mitomycin C (Ametycine) is an antineoplastic antibiotic by inhibiting DNA synthesis.
|Trametinib (GSK1120212; Jtp-74057) is an orally effective MEK inhibitor with IC50 of 2 nM against MEK1 and MEK2, respectively. Trametinib can activate autophagy and induce apoptosis.
|Doxorubicin HCl (Adriamycin, DOX, RP 13057) hydrochloride is an antibiotic-like agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis.
|GDC-0941 is a selective and potent inhibitor of Class I PI3K, p110a IC50=0.003uM, U87MG IC50=0.95μM.
|Hoechst 33342 trihydrochloride
|Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain. Hoechst binds to the grooves in the DNA double strand, which tends to be A/T-rich DNA strand. Although it binds to all nucleic acids, the A/T-rich double strand DNA significantly enhances fluorescence intensity Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
|Etoposide (VP-16-213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity.
|LY2109761 is a novel TGF-β receptor type I and type II dual inhibitor.
|Dabrafenib (free base) (GSK2118436A) is a V600 mutant BRAF-specific inhibitor.
|Calcein-am is a fluorescent dye that penetrates cells and is used to measure cell viability. The excitation and emission wavelengths of Calcein are 494 nm and 517 nm, respectively. Fluorescent green. Store at -20℃ away from light.
|Staurosporine (AM-2282) is a potent ATP-competitive kinase inhibitor with IC50 of 0.7, 7, 8.5, 6, 20 nM for PKC, PKA,PKG, p60v-src tyrosine protein kinase, CaM kinase II, respectively.
|Sulforaphane (SFN) extracted from broccoli sprout is an agent with potent anti-oxidant and anti-inflammatory activity.
|Ruxolitinib (INCB018424) is a first-in-class, potent, selective JAK1/2 inhibitor with an IC50 of 3.3 nM/2.8 nM. It is more than 130-fold more selective for JAK1, JAK2, and JAK3 than JAK3. It can be used in studies related to pemphigus, myelofibrosis and vitiligo.
|Succinimide (CFSE) is a luciferase - based tracer that penetrates and immobilizes cells for extremely long-term cell labeling. The maximum excitation/emission wavelength is 494nm/521 nm. Fluorescent green. Store away from light.
|Wortmannin (KY 12420)
|Wortmannin (KY 12420) is a specific, covalent inhibitor of phosphatidylinositol 3-kinase (PI 3-kinase) with IC50 of 3 nM.
|Sumatriptan succinate (GR 43175) is an orally active 5-HT1 receptor agonist with Kis of 17 nM, 27 nM and 100 nM for 5-HT1D, 5-HT1B and 5-HT1A receptors, respectively. Sumatriptan succinate can be used for the research of migraine headache.
|Hoechst 33342 (HOE 33342) is a cell membrane permeable nuclear staining reagent for DNA staining. The maximum excitation/emission wavelength is 361nm/497nm, and it gives blue fluorescence, which is often used to observe the nucleus/nucleolus. Store at 4°C away from light.
|ABT-263 (Navitoclax) is a potent, orally bioavailable inhibitor of the antiapoptotic Bcl-2 family members Bcl-2, Bcl-x (L), and Bcl-w.
|BAPTA-AM is a selective, membrane-permeable calcium chelator. BAPTA-AM inhibits hERG channels, hKv1.3 and hKv1.5 channels in HEK 293 cells with IC50s of 1.3 μM, 1.45 μM and 1.23 μM, respectively.
|Gefitinib (ZD1839) is an EGFR tyrosine kinase and Akt phosphorylation inhibitor. Gefitinib significantly down-regulates CD47 expression and increases DC phagocytosis in non-small cell lung cancer cells such as PC9, H1437 and H1573. Gefitinib combined with CD47 antibody can enhance the phagocytosis of macrophages.
|Fulvestrant is a Estrogen receptor (ER) antagonist with IC50 of 0.094 nM. Fulvestrant can also induce autophagy and apoptosis, and has anti-tumor effect.
|2-NBDG is a fluorescent glucose uptake indicator.
|BML-275 (Dorsomorphin) is a cell-permeable pyrazolopyrimidine compound shown to be an AMPK inhibitor with Ki of 109 nM in cell-free assays.
|ABT-199 is a highly potent, orally bioavailable and BCL-2 inhibitor. ABT-199 is the first inhibitor targeting Bcl-2 and PPI.
|Necrostatin-1 (NEC-1) is a potent inhibitor of necroptosis, with an EC50 of 490 nM in Jurkat cells. Necrostin-1 is the first identified RIPK1 inhibitor, EC50=182 nM, that reduces inflammation and inhibits colitis associated tumorigenesis. Necrostatin-1 (Nec-1) is also an inhibitor of IDO.
|Gemcitabine(LY-188011; NSC 613327) is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.
|Coelenterazine is the luciferin, the light-emitting molecule, used for monitoring reporter genes in BRET, ELISA and HTS techniques.*The compound is unstable in solutions, freshly prepared is recommended
|Insulin is a peptide hormone produced by beta cells of the pancreatic islets. The compound is unstable in solutions, freshly prepared is recommended.
|SBE-β-CD (Sulfobutylether beta-cyclodextrin)
|SBE-β-CD (Sulfobutylether beta-cyclodextrin) is a unique reproducible mixture of polyanionic β-cyclodextrin derivatives in which a sodium sulfonate salt is tethered to the lipophilic cyclodextrin cavity by a butyl ether group, or sulfobutylether (SBE).
|Carboplatin (NSC 241240) is a DNA synthesis inhibitor by binding to DNA and interfering with the cell's repair mechanism.
|Imatinib is a multi-target inhibitor of v-Abl, c-Kit and PDGFR with IC50 of 0.6 μM, 0.1 μM and 0.1 μM, respectively.
|BAY 11-7082 is an inhibitor of cytokine-induced IκB-α phosphorylation (IC50 ~ 10 μM). BAY 11-7082 inhibits ubiquitin-specific protease USP7 and USP21 (IC50=0.19, 0.96 μM, respectively).
|ICG-001 is a novel small molecule inhibitor of TCF/beta-catenin-mediated transcription.
|CX-4945 (Silmitasertib) is a potent small molecule protein kinase inhibitor that selectively binds to and inhibits the enzyme casein kinase II (CK2).
|Dithiothreitol (DTT) is a redox reagent commonly used as a reducing agent for thiolated DNA. Dithiothreitol (DTT) is also used to reduce the disulfide bonds of proteins.
|BEZ235 (NVP-BEZ235) is a potent dual PI3K and mTOR inhibitor of p110α, p110γ, p110δ and p110β with IC50 of 4 nM, 5 nM, 7 nM and 75 nM, respectively.
|Hoechst 33342 solution
|Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
|4-Phenylbutyric acid (4-PBA)
|4-Phenylbutyric acid is an inhibitor of HDAC, inhibits the growth of NSCLC Cell Lines at 2 mM.
|PD98059 is a potent and selective inhibitor of mitogen-activated protein kinase kinase (MKK / MEK) with IC50 value of 2-7 μM. The expression of KAT2B protein in cells is significantly decreased after PD98059 inhibits ERK signaling pathway.
|Decitabine (5-Aza-2'-deoxycytidine) is a potent inhibitor of DNA methylation for the treatment of myelodysplastic syndromes.
|CH-223191 is a potent and specific aryl hydrocarbon receptor (AhR) antagonist. CH-223191 inhibits TCDD-mediated nuclear translocation and DNA binding of AhR, and inhibits TCDD-induced luciferase activity with an IC50 of 0.03 μM. *The compound is unstable in solutions, freshly prepared is recommended
|Corn oil, extracted from the germ of corn, can be used as a carrier for drug molecules and has anti-tumor activity. It can be used to construct animal models of hypospadias.
|SB 202190 is a highly selective, potent and cell permeable inhibitor of p38 MAP kinase with IC50 values of 50 and 100 nM inhibiting p38α and p38β, respectively.
|Selisistat (EX 527)
|Selisistat (EX 527) is a potent and selective SIRT1 class III histone deacetylase enzyme inhibitor with an IC50 of 98 nM.
|D-Luciferin potassium salt
|D-Luciferin potassium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects.
|(+)-JQ1 is a BET bromodomain inhibitor that acts on BRD4(2) with an IC50 of 33 nM and binds to all bromodomain domains in the BET family, but not to bromodomain domains outside the BET family. JQ1 down-regulates CD47 expression through a C-MYC-mediated pathway, and JQ1 down-regulates CD47 expression through a C-MYC-mediated pathway.
|Bortezomib (PS-341) is a highly selective proteasome inhibitor with a ki value of 6nM.
|MHY1485 is an mTOR activator that potently inhibits autophagy by suppression of fusion between autophagosomes and lysosomes.
|Osimertinib (AZD-9291) is an oral, irreversible, and mutant-selective EGFR inhibitor with IC50 of 12.92, 11.44 and 493.8 nM for Exon 19 deletion EGFR, L858R/T790M EGFR, and WT EGFR, respectively.
|Insulin has anabolic properties that include the stimulation of both skeletal muscle and liver cells to incorporate glucose and convert it to glycogen, to synthesize proteins from amino acids in the blood, and to act on fat cells to enhance their uptake of glucose and the synthesis of fat.
|Carboxymethyl cellulose sodium
|Carboxymethyl cellulose sodium is frequently used as viscous agent, paste and barrier agent.
|EDTA disodium is a chelating agent.
|Rotenone is an inhibitor of mitochondrial electron transport at NADH:ubiquinone oxidoreductase, and is used to induce a Parkinson-like syndrome as an experimental model in rats.
|ZCL278 is a selective Cdc42 GTPase inhibitor with Kd of 11.4 μM.
|NSC 23766 is an inhibitor of Rac GTPase targeting Rac activation by guanine nucleotide exchange factors (GEFs) with IC50 of ~50 μM in a cell-free assay; does not inhibit the closely related targets, Cdc42 or RhoA.
|Perifosine is an orally active Akt inhibitor, the antiproliferative properties of Perifosine with an IC50 of 0.6–8.9 µM.
|Dexamethasone (Hexadecadrol) is a glucocorticoid receptor agonist that induces the production of phospholipase A2 inhibitory protein.It can be used to construct models of allergic conjunctivitis.
|Dynasore is a cell-permeable, reversible non-competitive dynamin inhibitor of GTPase activity of dynamin 1/2, with IC50 of 15 μM.
|Atezolizumab (atezolizumab) is a humanized anti-PD-L1 monoclonal antibody that inhibits PD-L1 signaling with PD-1 and B7-1 and restores tumor-specific T-cell immunity. However, it does not affect the interaction of PD-L2 with PD-1. MW: 145 KD. atezolizumab reacts with murine PD-L1 (for human and non-humanized mice).
|EDTA is a chelating agent. EDTA can activate NOTCH1 signaling.
|Simvastatin (MK 733)
|Simvastatin (MK-0733, MK 733) is a competitive inhibitor of HMG-CoA reductase with Ki of 0.1-0.2 nM in cell-free assays. Simvastatin induces ferroptosis, mitophagy, autophagy and apoptosis. Simvastatin inhibits exosome release. Prior to use in cell assays, Simvastatin needs to be activated by NaOH in EtOH treatment.
|D-Glucose (Glucose), a simple sugar (monosaccharide), is an important carbohydrate in biology.
|A 83-01 is a selective inhibitor of TGF-β type I receptor ALK5 kinase, type I activin/nodal receptor ALK4 and type I nodal receptor ALK7 (IC50 values are 12, 45 and 7.5 nM respectively).*The compound is unstable in solutions, freshly prepared is recommended
|GW9662 is a selective PPAR antagonist for PPARγ with IC50 of 3.3 nM, with at least 100 to 1000-fold functional selectivity in cells with PPARγ versus PPARα and PPARδ.
|Cytarabine (Cytosine arabinoside, AraC) is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM.
|CHIR-99021 HCl (CT99021) is a hydrochloride of CHIR-99021, with IC50 of 10 nM and 6.7 nM for GSK-3α and GSK-3β, respectively.
|Temsirolimus (WAY-130779, CCI-779) is a specific mTOR inhibitor with IC50 of 1.76 μM.
|MTT is a reagent used in the measurement of cell proliferation.
|Onx-0914 (PR-957) is a selective inhibitor of low molecular weight polypeptide 7 (LMP7), a chymotrypsin subunit of the immune proteasome. Onx-0914 blocks cytokine production and reduces the progression of experimental arthritis. Onx-0914 is a non-competitive irreversible inhibitor of mycobacterium proteasome (Ki=5.2 μM). Onx-0914 activates latent HIV-1 through HF-1-mediated p-TEFb activation.
|Forskolin (Coleonol) is a cell-permeable activator of adenylyl cyclase.
|VX-765 is a potent and selective inhibitor of caspase-1 with Ki of 0.8 nM.
|Tunicamycin is a mixture of nucleoside antibiotics that inhibits N-glycosylation and blocks GlcNAc phosphotransferase (GPT).
|AZD6244 (ARRY-142886, Selumetinib) is a potent MEK 1/2 inhibitor with GI50 values ranging from 14 to 50 nM.
|Indomethacin is a non-steroidal anti-inflammatory compound commonly used to reduce fever, pain, stiffness, and swelling.
|TH-302 is a highly potent and selective hypoxia-activated procompound targeting hypoxic regions of solid tumors with IC50 of 19 nM.
|IWP-L6 is a highly potent Porcn inhibitor with EC50 of 0.5 nM.
|Ionomycin (Calcium salt)
|Ionomycin is a calcium ionophor and an antibiotic produced by Streptomyces conglobatus.
|Cryptotanshinone is a potent anticancer agent targeting the activation STAT3 protein with antitumor activity.
|2-Deoxy-D-Glucose is a glucose analog that inhibits glycolysis via its actions on hexokinase, the rate limiting step of glycolysis. It is phosphorylated by hexokinase to 2-DG-P which cannot be further metabolized by phosphoglucose isomerase.
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