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MHY1485

Cat. No. M9050
MHY1485 Structure
Size Price Availability Quantity
10mM*1mL USD 45  USD50 In stock
5mg USD 36  USD40 In stock
10mg USD 63  USD70 In stock
50mg USD 220.5  USD245 In stock
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Quality Control
Biological Activity

MHY1485 induced mTOR activation and correspondingly showed a higher docking score than PP242, a well-known ATP-competitive mTOR inhibitor, in docking simulation. In conclusion, MHY1485 has an inhibitory effect on the autophagic process by inhibition of fusion between autophagosomes and lysosomes leading to the accumulation of LC3II protein and enlarged autophagosomes. MHY1485 also induces mTOR activity, providing a possibility for another regulatory mechanism of autophagy by the MHY compound. MHY1485 treatment increases ribosomal protein S6 kinase (S6K) and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) phosphorylation, which are downstream targets of mTOR complex 1 (mTORC1), but decreases phosphorylation of Akt on mTOR complex 2 (mTORC2) target site serine 473.

Product Citations
Customer Product Validations & Biological Datas
Source Life Sciences (2020 Jun). Figure 5. MHY1485 (Abmole Bioscience, US)
Method Proliferation analysis by CCK-8 method
Cell Lines Rat HSCs cell line
Concentrations 5 μM
Incubation Time 48 h
Results Moreover, both PLD1-I and PLD1-SiRNA could significantly blunt proliferation of HSC-T6 treated with MHY1485 (mTOR activator)
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 387.39
Formula C17H21N7O4
CAS Number 326914-06-1
Purity 99.33%
Solubility DMSO 7 mg/mL (Need ultrasonic and warming)
Storage at -20°C
References

Administration of follicle-stimulating hormone induces autophagy via upregulation of HIF-1α in mouse granulosa cells
Jilong Zhou, et al. Cell Death Dis. 2017 Aug 17;8(8):e3001. PMID: 28817115.

Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators.
Cheng Y, et al. PLoS One. 2015 Feb 24;10(2):e0117769. PMID: 25710488.

Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion.
Choi YJ, et al. PLoS One. 2012;7(8):e43418. PMID: 22927967.

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  Catalog
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Keywords: MHY1485 supplier, mTOR, inhibitors

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