Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain. Hoechst binds to the grooves in the DNA double strand, which tends to be A/T-rich DNA strand. Although it binds to all nucleic acids, the A/T-rich double strand DNA significantly enhances fluorescence intensity Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
*The compound is unstable in solutions, freshly prepared is recommended
Carbohydrate Polymers. 2023 Dec 1.
Chitosan-based nano-micelles for potential anti-tumor immunotherapy: Synergistic effect of cGAS-STING signaling pathway activation and tumor antigen absorption
Hoechst 33342 trihydrochloride purchased from AbMole
J Transl Med. 2023 Aug 12;21(1):540.
Decreased cyclooxygenase-2 associated with impaired megakaryopoiesis and thrombopoiesis in primary immune thrombocytopenia
Hoechst 33342 trihydrochloride purchased from AbMole
Gastric Cancer. 2020 Mar;23(2):241-259.
The modification of ferroptosis and abnormal lipometabolism through overexpression and knockdown of potential prognostic biomarker perilipin2 in gastric carcinoma.
Hoechst 33342 trihydrochloride purchased from AbMole
PLoS Pathog. 2021 Feb 8;17(2):e1008992.
CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreatitis
Hoechst 33342 trihydrochloride purchased from AbMole
Evid Based Complement Alternat Med. 2022 Apr 6;2022:4445293.
Zhenzhu Xiaoji Decoction Induces Autophagy and Apoptosis Cell Death in Liver Cancer Cells through AKT/mTOR and JAK2/STAT3 Signaling Pathway
Hoechst 33342 trihydrochloride purchased from AbMole
Front Oncol. 2023 Mar 29;13:1140256.
Near-infrared fluorescence imaging of hepatocellular carcinoma cells regulated by β-catenin signaling pathway
Hoechst 33342 trihydrochloride purchased from AbMole
| Molecular Weight | 561.93 |
| Formula | C27H31Cl3N6O |
| CAS Number | 875756-97-1 |
| Solubility (25°C) | DMSO ≥ 45 mg/mL Water ≥ 5.5 mg/mL |
| Storage | 2-8°C, protect from light, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] Brad Chazotte. Cold Spring Harb Protoc. Labeling nuclear DNA with hoechst 33342
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