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Hoechst 33342 trihydrochloride

Cat. No. M13565
Hoechst 33342 trihydrochloride Structure

bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride

Size Price Availability Quantity
25mg USD 48  USD48 In stock
50mg USD 78  USD78 In stock
100mg USD 115  USD115 In stock
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Quality Control & Documentation
Biological Activity

Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain. Hoechst binds to the grooves in the DNA double strand, which tends to be A/T-rich DNA strand. Although it binds to all nucleic acids, the A/T-rich double strand DNA significantly enhances fluorescence intensity Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.

*The compound is unstable in solutions, freshly prepared is recommended

Product Citations
Chemical Information
Molecular Weight 561.93
Formula C27H31Cl3N6O
CAS Number 875756-97-1
Solubility (25°C) DMSO ≥ 45 mg/mL
Water ≥ 5.5 mg/mL
Storage 2-8°C, protect from light, dry, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Ayad Eddaoudi, et al. Methods Mol Biol. Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining

[2] Lisa C Crowley, et al. Cold Spring Harb Protoc. Detection of DNA Fragmentation in Apoptotic Cells by TUNEL

[3] Lisa C Crowley, et al. Cold Spring Harb Protoc. Analyzing Cell Death by Nuclear Staining with Hoechst 33342

[4] Brad Chazotte. Cold Spring Harb Protoc. Labeling nuclear DNA with hoechst 33342

[5] Martin Purschke, et al. Photochem Photobiol Sci. Phototoxicity of Hoechst 33342 in time-lapse fluorescence microscopy

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Keywords: Hoechst 33342 trihydrochloride, bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride supplier, Fluorescent Dye, inhibitors, activators

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