Certificate of Analysis
| Product Name: | Hoechst 33342 trihydrochloride |
| Catalog Number: | M13565 |
| CAS Number: | 875756-97-1 |
Physical and Chemical Properties
| Formula: | C27H31Cl3N6O |
| Molecular Weight: | 561.93 |
| Solubility: | DMSO ≥ 45 mg/mL; Water ≥ 5.5 mg/mL |
| Storage: | 2-8°C, protect from light, dry, sealed |
| Chemical Structure: |  |
Analytical Data
| QC Standard: | >99% |
| H-NMR: | Consistent with structure |
Product Information
| Description: | Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain. Hoechst binds to the grooves in the DNA double strand, which tends to be A/T-rich DNA strand. Although it binds to all nucleic acids, the A/T-rich double strand DNA significantly enhances fluorescence intensity Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. |
| Stability and Solubility Advice: | Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details. |