Free shipping on all orders over $ 500

Actinomycin D

Cat. No. M4881
Actinomycin D Structure
Synonym:

Actinomycin Aiv; Dactinomycin; Act D; RASP-101

Size Price Availability Quantity
5mg USD 57  USD57 In stock
10mg USD 105  USD105 In stock
50mg USD 380  USD380 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

Actinomycin D is a compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy.

Product Citations
Customer Product Validations & Biological Datas
Source Theranostics (2020 Apr). Figure 5. Actinomycin D (Abmole Bioscience, US)
Method RNA decay assay
Cell Lines MHCC97H and HepG2 cells
Concentrations 5 μg/mL
Incubation Time -
Results We next sought to assess the RNA decay rate in Mettl3-WT and Mettl3-KR cells treated with the transcription inhibitor actinomycin D to block new RNA synthesis. Snail mRNA expression was decreased, and the Snail mRNA half-lives were significantly shortened in SUMO-mutant Mettl3 cells
Chemical Information
Molecular Weight 1255.41
Formula C62H86N12O16
CAS Number 50-76-0
Solubility (25°C) DMSO ≥ 30 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Heinicke U,et.al. Oncotarget. JNJ-26481585 primes rhabdomyosarcoma cells for chemotherapeutics by engaging the mitochondrial pathway of apoptosis.

[2] Anthony A Cuneo, et al. J Mol Cell Cardiol. Il-19 reduces VSMC activation by regulation of mRNA regulatory factor HuR and reduction of mRNA stability

[3] Elisabetta Bini, et al. RNA. Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricus

[4] S Li, et al. Toxicol Appl Pharmacol. Superinduction of IL-2 gene expression by vomitoxin (deoxynivalenol) involves increased mRNA stability

Related DNA/RNA Synthesis Products
3-Hydroxy-2-methylpyridine

3-Hydroxy-2-methylpyridine, isolated from alkaline extracts of cocoa, is used in the synthesis of pyrimidine.

DMT-dT-CE

DMT-dT-CE is typically used in the synthesis of DNA.

DMT-dC(Ac)-CE

DMT-dC(Ac)-CE is a modified phosphoramidite monomer, which can be used for the oligonucleotide synthesis.

DMT-dC(Bz)-CE

DMT-dC(Bz)-CE is typically used in the synthesis of DNA.

DMT-dG(dmf)-CE

DMT-dG(dmf)-CE is a phosphinamide monomer that can be used in the preparation of oligonucleotides.

  Catalog
Abmole Inhibitor Catalog




Keywords: Actinomycin D, Actinomycin Aiv; Dactinomycin; Act D; RASP-101 supplier, DNA/RNA Synthesis, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.