Actinomycin D is a compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy.
Cell Death Dis. 2023 Sep 21;14(9):619.
Interfering with Rac1-activation during neonatal monocyte-macrophage differentiation influences the inflammatory responses of M1 macrophages
Actinomycin D purchased from AbMole
Sci Total Environ. 2023 Sep 18;167202.
m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance
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BMC Complementary Medicine and Therapies. 2023 Jul 1.
NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression
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Clin Epigenetics. 2023 Jul 5;15(1):109.
FTO-dependent m6A regulates muscle fiber remodeling in an NFATC1–YTHDF2 dependent manner
Actinomycin D purchased from AbMole
J Transl Med. 2023 Jun 23;21(1):407.
METTL3-mediated m6A modification of SIRT1 mRNA inhibits progression of endometriosis by cellular senescence enhancing
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Plant Cell. 2023 Jun 27;koad189.
N4-acetylation of cytidine in (m) RNA plays essential roles in plants
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J Orthop Sci. 2023 May 27;S0949-2658(23)00133-1.
CircBBS9 accelerates the malignant progression of osteosarcoma through sponging miR-485-3p/HMGB1 axis
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Front Immunol. 2023 Mar 30;14:1144774.
LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma
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J Hazard Mater. 2023 May 6;455:131543.
BPDE exposure promotes trophoblast cell pyroptosis and induces miscarriage by up-regulating lnc-HZ14/ZBP1/NLRP3 axis
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Clin Epigenetics. 2023 Jul 5;15(1):109.
FTO-dependent m6A regulates muscle fiber remodeling in an NFATC1-YTHDF2 dependent manner
Actinomycin D purchased from AbMole
J Exp Clin Cancer Res. 2023 May 19;42(1):127.
Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m6A-dependent recognition of Nrf2 mRNA mediated by SND1
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J Cell Biochem. 2023 Feb;124(2):239-253.
IGF2BP2 enhances LincRNA01116 stability via m6A: A potential biomarker and therapeutic target for patients with pre‐eclampsia
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Biomed Pharmacother. 2023 May;161:114575.
Targeting the TNF-α–TNFR interaction with EGCG to block NF-κB signaling in human synovial fibroblasts
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Plant Biotechnol J. 2023 Mar 22.
N6‐methyladenosine RNA modification regulates cotton drought response in a Ca2+ and ABA‐dependent manner
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Molecules. 2022 May 16;27(10):3182.
A Novel Strategy for Regulating mRNA's Degradation via Interfering the AUF1's Binding to mRNA
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iScience. 2022 Nov 10;25(12):105552.
Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability
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Cells. 2022 Sep 27;11(19):3008.
m6A Modification of Long Non-Coding RNA HNF1A-AS1 Facilitates Cell Cycle Progression in Colorectal Cancer via IGF2BP2-Mediated CCND1 mRNA Stabilization
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Antiviral Res. 2023 Jan;209:105482.
Ciliatoside A, isolated from Peristrophe japonica, inhibits HBsAg expression and cccDNA transcription by inducing autophagy
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Int J Biol Sci. 2023 Jan 1;19(2):691-704.
Upregulation of LRRC8A by m5C modification-mediated mRNA stability suppresses apoptosis and facilitates tumorigenesis in cervical cancer
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Adv Sci (Weinh). 2023 Jan;10(2):e2201724.
Silencing of IRF8 Mediated by m6A Modification Promotes the Progression of T‐Cell Acute Lymphoblastic Leukemia
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Cell Biol Toxicol. 2022 Feb 28;1-17.
AS3MT facilitates NLRP3 inflammasome activation by m6A modification during arsenic-induced hepatic insulin resistance
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Mol Cancer. 2022 Feb 14;21(1):49.
Exosomal circLPAR1 functions in colorectal cancer diagnosis and tumorigenesis through suppressing BRD4 via METTL3–eIF3h interaction
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Cancer Lett. 2021 Dec 28;523:135-147.
IGFBP7-AS1 is a p53-responsive long noncoding RNA downregulated by Epstein-Barr virus that contributes to viral tumorigenesis
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Theranostics. 2020 Apr 27;10(13):5671-5686.
SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma
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Source | Theranostics (2020 Apr). Figure 5. Actinomycin D (Abmole Bioscience, US) |
| Method | RNA decay assay | |
| Cell Lines | MHCC97H and HepG2 cells | |
| Concentrations | 5 μg/mL | |
| Incubation Time | - | |
| Results | We next sought to assess the RNA decay rate in Mettl3-WT and Mettl3-KR cells treated with the transcription inhibitor actinomycin D to block new RNA synthesis. Snail mRNA expression was decreased, and the Snail mRNA half-lives were significantly shortened in SUMO-mutant Mettl3 cells |
| Molecular Weight | 1255.41 |
| Formula | C62H86N12O16 |
| CAS Number | 50-76-0 |
| Solubility (25°C) | DMSO ≥ 30 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related DNA/RNA Synthesis Products |
|---|
| 3-Hydroxy-2-methylpyridine
3-Hydroxy-2-methylpyridine, isolated from alkaline extracts of cocoa, is used in the synthesis of pyrimidine. |
| DMT-dT-CE
DMT-dT-CE is typically used in the synthesis of DNA. |
| DMT-dC(Ac)-CE
DMT-dC(Ac)-CE is a modified phosphoramidite monomer, which can be used for the oligonucleotide synthesis. |
| DMT-dC(Bz)-CE
DMT-dC(Bz)-CE is typically used in the synthesis of DNA. |
| DMT-dG(dmf)-CE
DMT-dG(dmf)-CE is a phosphinamide monomer that can be used in the preparation of oligonucleotides. |
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