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Actinomycin D is a compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy.
Molecules. 2022 May 16;27(10):3182.
A Novel Strategy for Regulating mRNA's Degradation via Interfering the AUF1's Binding to mRNA
Actinomycin D purchased from AbMole
iScience. 2022 Nov 10;25(12):105552.
Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability
Actinomycin D purchased from AbMole
Cells. 2022 Sep 27;11(19):3008.
m6A Modification of Long Non-Coding RNA HNF1A-AS1 Facilitates Cell Cycle Progression in Colorectal Cancer via IGF2BP2-Mediated CCND1 mRNA Stabilization
Actinomycin D purchased from AbMole
Antiviral Res. 2023 Jan;209:105482.
Ciliatoside A, isolated from Peristrophe japonica, inhibits HBsAg expression and cccDNA transcription by inducing autophagy
Actinomycin D purchased from AbMole
Int J Biol Sci. 2023 Jan 1;19(2):691-704.
Upregulation of LRRC8A by m5C modification-mediated mRNA stability suppresses apoptosis and facilitates tumorigenesis in cervical cancer
Actinomycin D purchased from AbMole
Adv Sci (Weinh). 2023 Jan;10(2):e2201724.
Silencing of IRF8 Mediated by m6A Modification Promotes the Progression of T‐Cell Acute Lymphoblastic Leukemia
Actinomycin D purchased from AbMole
Cell Biol Toxicol. 2022 Feb 28;1-17.
AS3MT facilitates NLRP3 inflammasome activation by m6A modification during arsenic-induced hepatic insulin resistance
Actinomycin D purchased from AbMole
Mol Cancer. 2022 Feb 14;21(1):49.
Exosomal circLPAR1 functions in colorectal cancer diagnosis and tumorigenesis through suppressing BRD4 via METTL3–eIF3h interaction
Actinomycin D purchased from AbMole
Cancer Lett. 2021 Dec 28;523:135-147.
IGFBP7-AS1 is a p53-responsive long noncoding RNA downregulated by Epstein-Barr virus that contributes to viral tumorigenesis
Actinomycin D purchased from AbMole
Theranostics. 2020 Apr 27;10(13):5671-5686.
SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma
Actinomycin D purchased from AbMole
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Source | Theranostics (2020 Apr). Figure 5. Actinomycin D (Abmole Bioscience, US) |
| Method | RNA decay assay | |
| Cell Lines | MHCC97H and HepG2 cells | |
| Concentrations | 5 μg/mL | |
| Incubation Time | - | |
| Results | We next sought to assess the RNA decay rate in Mettl3-WT and Mettl3-KR cells treated with the transcription inhibitor actinomycin D to block new RNA synthesis. Snail mRNA expression was decreased, and the Snail mRNA half-lives were significantly shortened in SUMO-mutant Mettl3 cells |
| Molecular Weight | 1255.41 |
| Formula | C62H86N12O16 |
| CAS Number | 50-76-0 |
| Solubility (25°C) | DMSO ≥ 30 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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