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Cat. No. M9096
H2DCFDA Structure

DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate

Size Price Availability Quantity
25mg USD 49.5  USD55 In stock
100mg USD 99  USD110 In stock
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Biological Activity

H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS).

ROS Measurements

For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA.

Product Citations
Customer Product Validations & Biological Datas
Source Comp Biochem Physiol C Toxicol Pharmacol (2021 Apr), Figure 3, H2DCFDA (Abmole Bioscience)
Method measure the ROS levels
Cell Lines Each C. elegans strain
Concentrations 50 μM
Incubation Time 1 h
Results The results showed that CEO exposure made no significant difference to the GSH amount in each worm, however, the lipid peroxidation degree decreased due to the reduced ROS levels.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 487.29
Formula C24H16Cl2O7
CAS Number 4091-99-0
Purity 99.61%
Solubility DMSO: ≥ 90 mg/mL
Storage -20°C, protect from light

Redox environment in stem and differentiated cells: A quantitative approach.
Lyublinskaya OG, et al. Redox Biol. 2017 Aug;12:758-769. PMID: 28426982.

Reactive Oxygen Species in Planarian Regeneration: An Upstream Necessity for Correct Patterning and Brain Formation
Nicky Pirotte, et al. Oxid Med Cell Longev. 2015;2015:392476. PMID: 26180588.

Sustained production of ROS triggers compensatory proliferation and is required for regeneration to proceed
Carole Gauron, et al. Sci Rep. 2013;3:2084. PMID: 23803955.

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Keywords: H2DCFDA, DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate supplier, Fluorescent Dye, inhibitors

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