Cisplatin

Biological Activity

Cisplatin is an inorganic platinum complex that inhibits DNA synthesis by forming DNA crosslinking agents, inactivates GPXs, reduces cell GSH and induces iron death in HCT116 and A549 cells.

DMSO can inactivate Cisplatin's activity!

Product Citations

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  bioRxiv. 2024 Jun 28;14(7):999.

  Functionality of BRCA1 supports the survival of prostate cancer cells during the development of castration resistance
  Cisplatin purchased from AbMole

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  Molecules. 2023 Mar 16;28(6):2685.

  Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities
  Cisplatin purchased from AbMole

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  J Immunother Cancer. 2022 Oct;10(10):e005270.

  CUL3/SPOP complex prevents immune escape and enhances chemotherapy sensitivity of ovarian cancer cells through degradation of PD-L1 protein
  Cisplatin purchased from AbMole

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  Sci Rep. 2022 Oct 17;12(1):17358.

  Screening of the siGPCR library in combination with cisplatin against lung cancers
  Cisplatin purchased from AbMole

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  Phytochemistry. 2022 Dec 12;207:113558.

  Diverse alkaloids from the aerial parts of Aconitum carmichaelii and antiproliferative activity of costemline via inhibiting SIRT1/ROCK1/P-STAT3 pathways
  Cisplatin purchased from AbMole

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  Evid Based Complement Alternat Med. 2022 Apr 6;2022:4445293.

  Zhenzhu Xiaoji Decoction Induces Autophagy and Apoptosis Cell Death in Liver Cancer Cells through AKT/mTOR and JAK2/STAT3 Signaling Pathway
  Cisplatin purchased from AbMole

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  Clin Epigenetics. 2021 Jul 22;13(1):142.

  Genome-wide DNA methylome analysis identifies methylation signatures associated with survival and drug resistance of ovarian cancers
  Cisplatin purchased from AbMole

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  Bioengineered. 2021 Dec;12(1):2499-2510.

  Targeting the miR-6734-3p/ZEB2 axis hampers development of non-small cell lung cancer (NSCLC) and increases susceptibility of cancer cells to cisplatin treatment
  Cisplatin purchased from AbMole

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  Aging (Albany NY). 2020 Jun 9;12(11):11025-11041.

  LncRNA ADAMTS9-AS2 inhibits gastric cancer (GC) development and sensitizes chemoresistant GC cells to cisplatin by regulating miR-223-3p/NLRP3 axis
  Cisplatin purchased from AbMole

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  Open Life Sciences. 2020 June;15(1):409-417.

  Buformin suppresses osteosarcoma via targeting AMPK signaling pathway
  Cisplatin purchased from AbMole

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  2020 Dec.

  A Novel Circular RNA hsa_circRNA_103809/miR-377-3p/GOT1 Pathway Regulates Cisplatin-Resistance in Non-Small Cell Lung Cancer (NSCLC)
  Cisplatin purchased from AbMole

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  Cell Cycle. 2018;17(10):1235-1244.

  Apatinib, a novel tyrosine kinase inhibitor, suppresses tumor growth in cervical cancer and synergizes with Paclitaxel.
  Cisplatin purchased from AbMole

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  Cancer Biol Ther. 2018 Feb 5.

  Buformin suppresses proliferation and invasion via AMPK/S6 pathway in cervical cancer and synergizes with paclitaxel
  Cisplatin purchased from AbMole

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  J Korean Med Sci. 2016 Jun;31(6): 836–842.

  Silencing of ABCG2 by MicroRNA-3163 Inhibits Multidrug Resistance in Retinoblastoma Cancer Stem Cells
  Cisplatin purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Cell Cycle (2018 Jul). Figure 5. Cisplatin (Abmole Bioscience)
	Method	cellular proliferation assay
	Cell Lines	SiHa cells
	Concentrations	5 μM
	Incubation Time	-
	Results	However, we found no synergy between Apatinib and cisplatin (CI ranged from 1.149 to 5.647 in SiHa, CI ranged from 0.85 to 1.682 in Hcc94) or carboplatin (CI ranged from 0.669 to 1.402 in SiHa and from 0.955 to 1.206 in Hcc94)

	Source	Cancer Biol Ther (2018). Figure 6. cisplatin (Abmole Bioscience, Shanghai, China)
	Method	Buformin synergizes with chemotherapy
	Cell Lines	C33A cells
	Concentrations	1 μM
	Incubation Time
	Results	In C33A cells, low-dose buformin (1μM) significantly enhanced the anti-proliferative effects when combined with cisplatin, paclitaxel, or 5-FU

	Source	J Korean Med Sci (2016). Figure 5. cisplatin, mitoxantrone and topotecan were all purchased from AbMole BioScience (Shanghai, China).
	Method	CCK8 Assay and ABCG2 ATPase assay
	Cell Lines	RCSC cells
	Concentrations	0~10 μM
	Incubation Time	72 h
	Results	MiR-3163 plays important roles in multidrug resistance (Table 1, Fig. 5A). Results showed that when miR-3163 was overexpressed in RCSCs, the ATPase activity of ABCG2 was significantly downregulated compared to RCSCs with Scramble (Fig. 5B).

	Source	J Korean Med Sci (2016). Figure 4. cisplatin, mitoxantrone and topotecan were all purchased from AbMole BioScience (Shanghai, China).
	Method	CCK8 Assay and flow cytometry analysis
	Cell Lines	RCSC cells
	Concentrations	0~10 μM
	Incubation Time	72 h
	Results	"A lower proliferation rate was observed in RCSCs transfected with miR-3163 Mimics using the CCK-8 assay (Fig. 4A).RCSCs treated with miR-3163 Mimics were more susceptible to cisplatin-induced apoptosis compared to those treated with Scramble (Fig.4B)."

Protocol (for reference only)

Cell Experiment
Cell lines	A549-luc cell
Preparation method	Cells were allowed to adhere for 24 hours and subsequently incubated with either PRINT-Platin or cisplatin at concentrations ranging from 9.8 nM to 80 µM (drug concentration) for 72 hours at 37 °C in a humidified 5 % CO2 atmosphere. After the incubation period, all media/particles were aspirated off cells. 100 µL fresh medium was added back to cells, followed by the addition of 100 µL CellTiter-Glo® Luminescent Cell Viability Assay reagent.
Concentrations	9.8 nM to 80 µM
Incubation time	72 h

Animal Experiment
Animal models	healthy mice
Formulation	pH-adjusted 0.9 wt% NaCl solution
Dosages	3 mg/kg
Administration	vein injection

Chemical Information

Molecular Weight	300.05
Formula	Cl2H6N2Pt
CAS Number	15663-27-1
Solubility (25°C)	DMF: 9 mg/mL (Need ultrasonic) Water ~1 mg/mL (ultrasonic and heating)
Storage	2-8°C, protect from light

References

[1] Kai MP, et al. J Control Release. Evaluation of drug loading, pharmacokinetic behavior, and toxicity of a cisplatin-containing hydrogel nanoparticle.

[2] Wilmes A, et al. Toxicol In Vitro. Mechanism of cisplatin proximal tubule toxicity revealed by integrating transcriptomics, proteomics, metabolomics and biokinetics.

[3] Matthew D Hall, et al. Cancer Res. Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes