Rapamycin (Sirolimus,Rapamune)is an inhibitor of mTOR.The mammalian target of rapamycin (mTOR) is a kinase responsible for mitogen-induced cell proliferation/survival signaling.In vitro, rapamycin inhibited the proliferation of primary bone marrow cells induced by IL-3, GM-CSF, KL, or a complex mixture of factors present in cell-conditioned media.
Int J Case Rep Orthop. 2021;3(2):129-134.
Rapamycin's osteogenic effects on bone marrow mesenchymal stem cells through triggering autophagy
Rapamycin purchased from AbMole
Front Immunol. 2023 Mar 14;14:1120996.
ROS-AMPK/mTOR-dependent enterocyte autophagy is involved in the regulation of Giardia infection-related tight junction protein and nitric oxide levels
Rapamycin purchased from AbMole
Arch Oral Biol. 2023 Apr 18;151:105700.
Wrinkled topography regulates osteogenesis via autophagy-mediated Wnt/β-catenin signaling pathway in MC3T3-E1 cells
Rapamycin purchased from AbMole
Commun Biol. 2023 Apr 3;6(1):361.
WSSV exploits AMPK to activate mTORC2 signaling for proliferation by enhancing aerobic glycolysis
Rapamycin purchased from AbMole
Adv Sci (Weinh). 2023 Mar 27;e2207224.
Hypoxia Drives Material‐Induced Heterotopic Bone Formation by Enhancing Osteoclastogenesis via M2/Lipid‐Loaded Macrophage Axis
Rapamycin purchased from AbMole
J Orthop Surg Res. 2023 Feb 22;18(1):129.
Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy
Rapamycin purchased from AbMole
Exp Cell Res. 2022 Nov 9;422(1):113414.
Gastric cancer-derived mesenchymal stem cells promote gastric cancer cell lines migration by modulating CD276 expression
Rapamycin purchased from AbMole
Cell Cycle. 2022 Sep 19;1-23.
Mechanistic target of rapamycin (mTOR) regulates self-sustained quiescence, tumor indolence and late clinical metastasis in a Beclin-1-dependent manner.
Rapamycin purchased from AbMole
CNS Neurosci Ther. 2022 Nov 24.
Acupuncture promotes nerve repair through the benign regulation of mTOR-mediated neuronal autophagy in traumatic brain injury rats
Rapamycin purchased from AbMole
Immunology. 2022 Sep 25.
mTORC1-GLUT1-mediated glucose metabolism drives hyperactivation of B cells in primary Sjogren's syndrome
Rapamycin purchased from AbMole
Mol Psychiatry. 2022 Jul 15;1-14.
Social isolation reinforces aging-related behavioral inflexibility by promoting neuronal necroptosis in basolateral amygdala
Rapamycin purchased from AbMole
Metabolism. 2023 Jan 7;140:155398.
Metformin coordinates with mesenchymal cells to promote VEGF-mediated angiogenesis in diabetic wound healing through Akt/mTOR activation
Rapamycin purchased from AbMole
J Hepatol. 2022 Nov 9;S0168-8278(22)03285-8.
Hepatocyte-specific Mas activation enhances lipophagy and fatty acid oxidation to protect against acetaminophen-induced hepatotoxicity in mice
Rapamycin purchased from AbMole
Mol Cancer. 2022 Mar 18;21(1):77.
Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression
Rapamycin purchased from AbMole
Oxid Med Cell Longev. 2021 Jun 14;2021:6662225.
Impaired Autophagy Induced by oxLDL/ β 2GPI/anti- β 2GPI Complex through PI3K/AKT/mTOR and eNOS Signaling Pathways Contributes to Endothelial Cell Dysfunction
Rapamycin purchased from AbMole
Genes & Diseases. 2020 Jun.
Metformin ameliorates HMGB1-mediated oxidative stress through mTOR pathway in experimental periodontitis
Rapamycin purchased from AbMole
Mol Med Rep. 2016 Sep;14(3):2194-8.
Anti‑migratory effect of rapamycin impairs allograft imaging by 18F‑fluorodeoxyglucose‑labeled splenocytes
Rapamycin purchased from AbMole
2015 Apr.
THE MECHANOBIOLOGICAL MECHANISMS OF CAVEOLAE/CAVEOLIN-1 IN LOW SHEAR STRESS-INDUCED BREAST CARCINOMA CELL MIGRATION AND INVASION
Rapamycin purchased from AbMole
Biomed Res Int. 2020 Jun 20;2020:9243681.
Shenmai Injection Supresses Glycolysis and Enhances Cisplatin Cytotoxicity in Cisplatin-Resistant A549/DDP Cells via the AKT-mTOR-c-Myc Signaling Pathway
Rapamycin purchased from AbMole
Oncogene. 2020 Apr;39(15):3163-3178.
The EGFR-ZNF263 Signaling Axis Silences SIX3 in Glioblastoma Epigenetically
Rapamycin purchased from AbMole
Cell Commun Signal. 2020 Feb 17;18(1):27.
mTOR May Interact With PARP-1 to Regulate Visible Light-Induced Parthanatos in Photoreceptors
Rapamycin purchased from AbMole
Mol Immunol. 2020 Jan;117:12-19.
Toll-like receptor 4 knockout protects against diabetic-induced imbalance of bone metabolism via autophagic suppression.
Rapamycin purchased from AbMole
Biosci Rep. 2018 Nov 20;38(6).
Rapamycin attenuates mitochondrial injury and renal tubular cell apoptosis in experimental contrast-induced acute kidney injury in rats.
Rapamycin purchased from AbMole
BMC Genomics. 2017 Sep 11;18(1):714.
The dynamic landscape of gene regulation during Bombyx mori oogenesis
Rapamycin purchased from AbMole
Oncotarget. 2016 Feb 22.
Mechanosensitive caveolin-1 activation-induced PI3K/Akt/mTOR signaling pathway promotes breast cancer motility, invadopodia formation and metastasis in vivo.
Rapamycin purchased from AbMole
APMIS. 2015 Jul;562-70.
MicroRNA-218 increases cellular sensitivity to Rapamycin via targeting Rictor in cervical cancer.
Rapamycin purchased from AbMole
Cell Experiment | |
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Cell lines | HeLa, SiHa, Caski, and C33A cells |
Preparation method | MTT assay. In brief, 2*103 cells/well were seeded into 96-well plates and routinely cultured overnight. Then, these cells were treated with Rapamycin for 72 h. Next, 5 µL MTT (5 mg/ mL; Sigma-Aldrich) and 200 µL DMSO were sequentially added into each well and the absorbance was measured at the wavelength of 570 nm. |
Concentrations | 0~80nM |
Incubation time | 72 h |
Animal Experiment | |
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Animal models | cervical cancer C33A cells tumor bearing mice |
Formulation | a solution of 0.2%CMC and 0.25% Tween-80 in ddH2O |
Dosages | 2.5 mg/kg from the 10th day to 37th day |
Administration | intra-peritoneal injection |
Molecular Weight | 914.18 |
Formula | C51H79NO13 |
CAS Number | 53123-88-9 |
Solubility (25°C) | DMSO 80 mg/mL |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] Zapata G, et al. Ocul Immunol Inflamm. Topical use of rapamycin in herpetic stromal keratitis.
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