All AbMole products are for research use only, cannot be used for human consumption.
Indomethacin is a potent and nonselective inhibitor of COX1 and COX2, with IC50s of 18 nM and 26 nM for human COX-1 and COX-2, respectively, in CHO cells. Indomethacin inhibits lipopolysaccharide (LPS)-induced PGE2 production (COX-2) in a human whole blood assay with a potency (IC50=0.68±0.17 μM), and suppresses coagulation-induced TXB2 production (COX-1) (IC50=0.19±0.02 μM). Indomethacin blocks COX-1 with an IC50 of 20±1 nM in U937 cell microsomes at a low arachidonic acid concentration (0.1 μM).
Food Res Int. 2024 Jul 30.
Optimization of differentiation conditions for porcine adipose-derived mesenchymal stem cells and analysis of fatty acids in cultured fat
Indomethacin purchased from AbMole
MedComm (2020). 2023 Sep 20;4(5):e363.
Arachidonic acid metabolism in health and disease
Indomethacin purchased from AbMole
Food Res Int. 2023 Jan 17;112486.
Evaluation the food safety of cultured fat via detection of residues of adipogenic differentiation cocktail in cultured fat with high performance liquid chromatography and enzyme-linked immunosorbent assay
Indomethacin purchased from AbMole
J Agric Food Chem. 2023 Feb 24.
Preparation and Quality Evaluation of Cultured Fat
Indomethacin purchased from AbMole
J Cosmet Dermatol. 2023 Jul 10.
Potent pigmentation inhibitory activity of incensole‐enriched frankincense volatile oil‐identification, efficacy and mechanism
Indomethacin purchased from AbMole
| Cell Experiment | |
|---|---|
| Cell lines | GES-1 cells |
| Preparation method | Viability assay Cellular proliferative viability was detected using the MTT assay. Briefly, GES-1 cells (1 × 10^5 cells/well) were seeded in a 96-well plate to adhere for 24 h, and then pretreated with oleanolic acid, ursolic acid, 3-O-acetyl ursolic acid, betulinic acid, 3-O-acetyl pomolic acid, maslinic acid, and TA (1.563, 3.125, 6.25, 12.5, 25, and 50 µM) for 6 h, then continued or treated with indomethacin (IND) in the concentration of 700 μM for 18 h. Next MTT solution (20 µL, 5 mg/mL) was added to each well and incubated for 4 h at 37 °C. Then the medium was discarded and 150 mL DMSO was added to dissolve the formazan crystals. Finally, viable cells were detected by measuring absorbance at 570 nm using a Microplate Reader. |
| Concentrations | 700 μM |
| Incubation time | 18 h |
| Animal Experiment | |
|---|---|
| Animal models | Male Sprague-Dawley rats (6 weeks old) |
| Formulation | 0.5% carboxymethylcellulose sodium |
| Dosages | 100 mg/kg |
| Administration | gavage |
| Molecular Weight | 357.79 |
| Formula | C19H16ClNO4 |
| CAS Number | 53-86-1 |
| Solubility (25°C) | DMSO ≥ 80 mg/mL |
| Storage | 2-8°C, protect from light, sealed |
| Related COX Products |
|---|
| Sudoxicam
Sudoxicam is a reversible and orally active COX antagonist and a non-steroidal anti-inflammatory agent from the enol-carboxamide class. |
| Metyrosine
Metyrosine is a selective tyrosine hydroxylase enzyme inhibitor. Metyrosine significantly inhibits high COX-2 activity. Metyrosine exerts anti-inflammatory and anti-ulcerative effects. |
| PPOH
PPOH, a fatty acid derivative, is a selective cyclooxygenase (COX) inhibitor that inhibits arachidonic acid cyclooxygenase activity in renal cortical microsomes. |
| Tenidap
Tenidap, a non-steroidal anti-inflammatory drug, is a selective COX-1 inhibitor, with IC50 values of 0.03 μM and 1.2 μM for COX-1 and COX-2, respectively. |
| COX-2-IN-6
COX-2-IN-6 is an orally active, gut-restricted and selective cyclooxygenase-2 (COX-2) inhibitor for colorectal Chemoprevention of cancer. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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