3-Methyladenine is a selective autophagy and phosphoinositide 3-kinase (PI3K) inhibitor for Vps34 and PI3Kγ with IC50 of 25 and 60 nM, respectively. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K), the activity of which is required for the nucleation and assembly of the initial phagophore membrane. 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the SAH group.
Oxid Med Cell Longev. 2021 Jun 14;2021:6662225.
Impaired Autophagy Induced by oxLDL/ β 2GPI/anti- β 2GPI Complex through PI3K/AKT/mTOR and eNOS Signaling Pathways Contributes to Endothelial Cell Dysfunction
3-Methyladenine (3-MA) purchased from AbMole
BMC Cancer. 2021 Feb 25;21(1):195.
Blockage of AMPK-ULK1 pathway mediated autophagy promotes cell apoptosis to increase doxorubicin sensitivity in breast cancer (BC) cells: an in vitro study
3-Methyladenine (3-MA) purchased from AbMole
Int J Neurosci. 2020 Mar 11;1-15.
Autophagy-deficiency in Bone Marrow Mononuclear Cells From Patients With Myasthenia Gravis: A Possible Mechanism of Pathogenesis.
3-Methyladenine (3-MA) purchased from AbMole
Front Pharmacol 2018 May 14;14;9:334.
Resveratrol Sensitizes Carfilzomib-Induced Apoptosis via Promoting Oxidative Stress in Multiple Myeloma Cells
3-Methyladenine (3-MA) purchased from AbMole
Mol Immunol. 2017 Jul;87:132-140.
IL-37 induces autophagy in hepatocellular carcinoma cells by inhibiting the PI3K/AKT/mTOR pathway
3-Methyladenine (3-MA) purchased from AbMole
Int J Clin Exp Med. 2017;10(5):7553-7562.
Combination of icotinib and wogonin induces apoptosis and autophagy to overcome acquired resistance in lung cancer harbouring EGFR T790M mutation
3-Methyladenine (3-MA) purchased from AbMole
Cell Experiment | |
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Cell lines | U251 cells |
Preparation method | Western blotting was performed to determine the expression levels of ubiquitinated proteins, PDI and Grp78. The expression levels of ubiquitinated PDI and Grp78 were increased following the inhibition of autophagy by treatment with 3-MA |
Concentrations | 10 mM |
Incubation time | 12 h |
Animal Experiment | |
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Animal models | Male Wistar rats |
Formulation | saline |
Dosages | 3.5 mg/kg |
Administration | i.v. |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
Molecular Weight | 149.15 |
Formula | C6H7N5 |
CAS Number | 5142-23-4 |
Purity | 100.0% |
Solubility | Water 6 mg/mL warmed |
Storage | at -20°C |
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