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3-Methyladenine (3-MA)

Cat. No. M2296

All AbMole products are for research use only, cannot be used for human consumption.

3-Methyladenine (3-MA) Structure
Synonym:

3-MA

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
25mg USD 36  USD36 In stock
50mg USD 50  USD50 In stock
100mg USD 80  USD80 In stock
200mg USD 130  USD130 In stock
500mg USD 260  USD260 In stock
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Biological Activity

3-Methyladenine is a selective autophagy and phosphoinositide 3-kinase (PI3K) inhibitor for Vps34 and PI3Kγ with IC50 of 25 and 60 nM, respectively. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K), the activity of which is required for the nucleation and assembly of the initial phagophore membrane. 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the SAH group.

Product Citations
Customer Product Validations & Biological Datas
Source Front Pharmacol (2018). Figure 6. 3-methyladenine (AbMole BioScience, Houston, TX, USA)
Method Intracellular ROS Production
Cell Lines MM cells
Concentrations 1 mmol/L
Incubation Time 2 h
Results However, RSV/CFZ combination increased levels of LC3-II and p62/SQSTM1. Higher levels of LC3-II could be partially blocked by the autophagy inhibitor 3-MA, but not for p62/SQSTM1
Source Int J Clin Exp Med (2017) . Figure 6. 3-methyladenine (3-MA) (Abmole Bioscience Inc. Houston, TX, USA)
Method Evaluation of the inhibitory activity
Cell Lines Human NSCLC cell line NCI-H1975
Concentrations 100 mM
Incubation Time
Results In addition, the suppression of mTOR phosphorylation decreased the percentage of early apoptotic cells (Figure 6A and 6B) and caspase 3 cleavage (Figure 6C).
Source Int J Clin Exp Med (2017) . Figure 5. 3-methyladenine (3-MA) (Abmole Bioscience Inc. Houston, TX, USA)
Method Evaluation of the inhibitory activity
Cell Lines Human NSCLC cell line NCI-H1975
Concentrations 100 mM
Incubation Time
Results As a control, 3-MA was used to prevent mTOR phosphorylation. The suppression of mTOR phosphorylation decreased the number of intracellular autophagosomes increased by treatment with wogonin plus icotinib combination (Figure 5B).
Source Mol Immunol (2017) . Figure 2. 3-methyladenine (Abmole Bioscience, USA)
Method IL-37 induces autophagy in SMMC-7721 and Huh-7 cells.
Cell Lines SMMC-7721 and Huh-7 cells
Concentrations 5 mmol/l
Incubation Time
Results The LC3-II/LC3-I ratio was significantly decreased in IL-37/3-MA-co-treated SMMC-7721 and Huh-7 cells and increased in IL-37/CQ-co-treated SMMC-7721 and Huh-7 cell (Fig. 2E and F). Together, these data further indicate that IL-37 induces autophagy in SMMC-7721 and Huh-7 cells.
Protocol (for reference only)
Cell Experiment
Cell lines U251 cells
Preparation method Western blotting was performed to determine the expression levels of ubiquitinated proteins, PDI and Grp78. The expression levels of ubiquitinated PDI and Grp78 were increased following the inhibition of autophagy by treatment with 3-MA
Concentrations 10 mM
Incubation time 12 h
Animal Experiment
Animal models Male Wistar rats
Formulation saline
Dosages 3.5 mg/kg
Administration i.v.
Chemical Information
Molecular Weight 149.15
Formula C6H7N5
CAS Number 5142-23-4
Solubility (25°C) Water 5 mg/mL warmed
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Peng Chen, et al. Ecotoxicol Environ Saf. The regulatory role of COX-2 in the interaction between Cr(VI)-induced endoplasmic reticulum stress and autophagy in DF-1 cells

[2] Dae Wook Hwang, et al. Pancreas. Autophagy Induced by CX-4945, a Casein Kinase 2 Inhibitor, Enhances Apoptosis in Pancreatic Cancer Cell Lines

[3] Zhang R, et al. Mol Med Rep. Inhibition of autophagy using 3-methyladenine increases cisplatin-induced apoptosis by increasing endoplasmic reticulum stress in U251 human glioma cells.

[4] Zhu B, et al. Int J Clin Exp Med. 3-methyladenine, an autophagic inhibitor, attenuates therapeutic effects of sirolimus on scopolamine-induced cognitive dysfunction in a rat model.

[5] Hualei Guo, et al. Cardiovasc Drυgs Ther. Resveratrol protects HUVECs from oxidized-LDL induced oxidative damage by autophagy upregulation via the AMPK/SIRT1 pathway

[6] Ying Xie, et al. Mol Med Rep. Protective role of autophagy in AGE-induced early injury of human vascular endothelial cells

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Keywords: 3-Methyladenine (3-MA), 3-MA supplier, PI3K, inhibitors, activators

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