Coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 μM/ml) shows a bioluminescence signal.
In vivo, in 4T1-implanted mouse, Coelenterazine detects cancer-associated superoxide anion and used to evaluate the role of superoxide anion in cancer cell biology and its role in pathology.
*The compound is unstable in solutions, freshly prepared is recommended
Cell Res. 2023 Jun;33(6):464-478.
SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches
Coelenterazine purchased from AbMole
J Transl Med. 2023 Jun 12;21(1):383.
Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer
Coelenterazine purchased from AbMole
Horticulture Research. 2023 Mar 28.
CaREM1.4 interacts with CaRIN4 to regulate Ralstonia solanacearum tolerance by triggering cell death in pepper
Coelenterazine purchased from AbMole
Plant Physiol. 2022 Jun 27;189(3):1380-1396.
TaBln1, a member of the Blufensin family, negatively regulates wheat resistance to stripe rust by reducing Ca2+ influx
Coelenterazine purchased from AbMole
J Nanobiotechnology. 2021 Nov 25;19(1):391.
Nanovesicles derived from bispecific CAR-T cells targeting the spike protein of SARS-CoV-2 for treating COVID-19
Coelenterazine purchased from AbMole
Molecular Weight | 423.46 |
Formula | C26H21N3O3 |
CAS Number | 55779-48-1 |
Solubility (25°C) | Ethanol 2 mg/mL (Need ultrasonic DMSO can inactivate Coelenterazine's activity) |
Storage | -20°C, protect from light, sealed |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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