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Carboplatin

Cat. No. M2288
Carboplatin Structure
Synonym:

Paraplatin

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
50mg USD 31.5  USD35 In stock
100mg USD 45  USD50 In stock
200mg USD 76.5  USD85 In stock
500mg USD 130.5  USD145 In stock
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Quality Control
Biological Activity

Carboplatin is a second-generation platinum compound with a broad spectrum of antineoplastic properties. Carboplatin is activated intracellularly to form reactive platinum complexes that bind to nucleophilic groups such as GC-rich sites in DNA, thereby inducing intrastrand and interstrand DNA cross-links, as well as DNA-protein cross-links. Carboplatin possesses tumoricidal activity similar to that of its parent compound, cisplatin, but is more stable and less toxic. Carboplatin is used against some forms of cancer (mainly ovarian carcinoma, lung, head and neck cancers). 

DMSO can inactivate Carboplatin's activity

Product Citations
Customer Product Validations & Biological Datas
Source Cell Cycle (2018 Jul). Figure 5. Carboplatin (Abmole Bioscience)
Method cellular proliferation assay
Cell Lines SiHa cells
Concentrations 5 μM
Incubation Time -
Results However, we found no synergy between Apatinib and cisplatin (CI ranged from 1.149 to 5.647 in SiHa, CI ranged from 0.85 to 1.682 in Hcc94) or carboplatin (CI ranged from 0.669 to 1.402 in SiHa and from 0.955 to 1.206 in Hcc94)
Source Cell Death Dis (2018). Figure 3. Carboplatin (Abmole Bioscience)
Method Glucose starvation sensitizes glioblastoma cells to chemotherapies
Cell Lines U87 and U251 cells
Concentrations 50 μM
Incubation Time 5 d
Results Treatment with either temozolomide or carboplatin induced about 50 and 80% GBM cell death under normal and glucose starvation conditions, respectively
Source Cell Death Dis (2018). Figure 1. Carboplatin (Abmole Bioscience)
Method Glucose starvation sensitizes glioblastoma cells to chemotherapies
Cell Lines U87 and U251 cells
Concentrations 50 μM
Incubation Time 5 d
Results The cytotoxic effect was progressive and by day 5, temozolomide or carboplatin treatment caused 40–60% cell loss in U87 and U251 cells. Glucose starvation nearly doubled the cell loss to 70–90%.
Source Mol Oncol (2014). Figure 4. Carboplatin
Method s.c.
Cell Lines Female 6-week-old nude mice
Concentrations 100 mg/kg
Incubation Time 5 weeks
Results Both carboplatin treatment groups showed significantly reduced tumor burden as compared to the untreated controls from weeks 4 to 10 (P < 0.0001)
Protocol
Cell Experiment
Cell lines 5637 cells
Preparation method Cells were dosed and incubated with 290 μM carboplatin or 290 μM carboplatin combined with 0.08 μM paclitaxel for 4 h, each including 0.43 μM[14C]carboplatin (106 dpm/ml). After washing, cells were maintained with 14C carboplatin-free culture media. DNA was extracted from cells and converted to graphite and measured by AMS for 14C quantification as previously described.
Concentrations 290 μM
Incubation time 4 h
Animal Experiment
Animal models Female 6‐week‐old nude mice
Formulation
Dosages 100 mg/kg
Administration s.c.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 371.25
Formula C6H12N2O4Pt
CAS Number 41575-94-4
Purity >98%
Solubility Water 4 mg/mL warmed
Storage 4°C, protect from light, dry, sealed
References

Paclitaxel Enhances Carboplatin-DNA Adduct Formation and Cytotoxicity.
Jiang S, et al. Chem Res Toxicol. 2015 Dec 21;28(12):2250-2. PMID: 26544157.

Enhancement of paclitaxel and carboplatin therapies by CCL2 blockade in ovarian cancers.
Moisan F, et al. Mol Oncol. 2014 Oct;8(7):1231-9. PMID: 24816187.

Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes
Matthew D Hall, et al. Cancer Res. 2014 Jul 15;74(14):3913-22. PMID: 24812268.

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  Catalog
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Keywords: Carboplatin, Paraplatin supplier, DNA/RNA Synthesis, inhibitors

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