Free shipping on all orders over $ 500

Carboplatin

Cat. No. M2288
Carboplatin Structure
Synonym:

Paraplatin

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
50mg USD 35  USD35 In stock
100mg USD 50  USD50 In stock
200mg USD 85  USD85 In stock
500mg USD 145  USD145 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
Biological Activity

Carboplatin is a second-generation platinum compound with a broad spectrum of antineoplastic properties. Carboplatin is activated intracellularly to form reactive platinum complexes that bind to nucleophilic groups such as GC-rich sites in DNA, thereby inducing intrastrand and interstrand DNA cross-links, as well as DNA-protein cross-links. Carboplatin possesses tumoricidal activity similar to that of its parent compound, cisplatin, but is more stable and less toxic. Carboplatin is used against some forms of cancer (mainly ovarian carcinoma, lung, head and neck cancers). 

DMSO can inactivate Carboplatin's activity

Product Citations
Customer Product Validations & Biological Datas
Source Cell Cycle (2018 Jul). Figure 5. Carboplatin (Abmole Bioscience)
Method cellular proliferation assay
Cell Lines SiHa cells
Concentrations 5 μM
Incubation Time -
Results However, we found no synergy between Apatinib and cisplatin (CI ranged from 1.149 to 5.647 in SiHa, CI ranged from 0.85 to 1.682 in Hcc94) or carboplatin (CI ranged from 0.669 to 1.402 in SiHa and from 0.955 to 1.206 in Hcc94)
Source Cell Death Dis (2018). Figure 3. Carboplatin (Abmole Bioscience)
Method Glucose starvation sensitizes glioblastoma cells to chemotherapies
Cell Lines U87 and U251 cells
Concentrations 50 μM
Incubation Time 5 d
Results Treatment with either temozolomide or carboplatin induced about 50 and 80% GBM cell death under normal and glucose starvation conditions, respectively
Source Cell Death Dis (2018). Figure 1. Carboplatin (Abmole Bioscience)
Method Glucose starvation sensitizes glioblastoma cells to chemotherapies
Cell Lines U87 and U251 cells
Concentrations 50 μM
Incubation Time 5 d
Results The cytotoxic effect was progressive and by day 5, temozolomide or carboplatin treatment caused 40–60% cell loss in U87 and U251 cells. Glucose starvation nearly doubled the cell loss to 70–90%.
Source Mol Oncol (2014). Figure 4. Carboplatin
Method s.c.
Cell Lines Female 6-week-old nude mice
Concentrations 100 mg/kg
Incubation Time 5 weeks
Results Both carboplatin treatment groups showed significantly reduced tumor burden as compared to the untreated controls from weeks 4 to 10 (P < 0.0001)
Protocol
Cell Experiment
Cell lines 5637 cells
Preparation method Cells were dosed and incubated with 290 μM carboplatin or 290 μM carboplatin combined with 0.08 μM paclitaxel for 4 h, each including 0.43 μM[14C]carboplatin (106 dpm/ml). After washing, cells were maintained with 14C carboplatin-free culture media. DNA was extracted from cells and converted to graphite and measured by AMS for 14C quantification as previously described.
Concentrations 290 μM
Incubation time 4 h
Animal Experiment
Animal models Female 6‐week‐old nude mice
Formulation
Dosages 100 mg/kg
Administration s.c.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 371.25
Formula C6H12N2O4Pt
CAS Number 41575-94-4
Purity >98%
Solubility Water 4 mg/mL warmed
Storage 4°C, protect from light, dry, sealed
References

[1] Jiang S, et al. Chem Res Toxicol. Paclitaxel Enhances Carboplatin-DNA Adduct Formation and Cytotoxicity.

[2] Moisan F, et al. Mol Oncol. Enhancement of paclitaxel and carboplatin therapies by CCL2 blockade in ovarian cancers.

[3] Matthew D Hall, et al. Cancer Res. Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes

Related DNA/RNA Synthesis Products
Tempo

Tempo is a nitric oxide radical and a selective scavenging agent of ROS that disambiguates superoxides in catalytic cycles. Tempo can induce DNA strand breaking and can be used as an organic catalyst for the oxidation of primary alcohols to aldehydes. Tempo has mutagenic and antioxidant effects.

NSC 617145

NSC 617145 is a selective werner syndrome helicase (WRN) helicase inhibitor,IC50 The value is 230 nM. NSC 617145 inhibit WRN ATPase and induce double-strand rupture (DSB) and chromosomal abnormalities. NSC 617145 is selective to WRN and superior to BLM, FANCJ, ChlR1, RecQ, and UvrD helicases.

IMP-1088

IMP-1088 is a novel potent and selective blocker of N-myristoylation in cells. IMP-1088 is also a potent human N-myristoyltransferases NMT1 and NMT2 dual inhibitor with IC50s of <1 nM for HsNMT1 and HsNMT2.

Methoxyamine HCl

Methoxyamine is an orally bioavailable small molecule inhibitor with potential adjuvant activity. Methoxyamine covalently binds to apurinic/apyrimidinic (AP) DNA damage sites and inhibits base excision repair (BER), which may result in an increase in DNA strand breaks and apoptosis.

RK-33

RK-33 is a first-in-class, potent and selective DDX3 (RNA helicase) inhibitor, it binds to DDX3 and abrogates its activity.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Carboplatin, Paraplatin supplier, DNA/RNA Synthesis, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.