Carboplatin (NSC 241240) is a second-generation platinum compound with a broad spectrum of antineoplastic properties. Carboplatin is activated intracellularly to form reactive platinum complexes that bind to nucleophilic groups such as GC-rich sites in DNA, thereby inducing intrastrand and interstrand DNA cross-links, as well as DNA-protein cross-links. Carboplatin possesses tumoricidal activity similar to that of its parent compound, cisplatin, but is more stable and less toxic. Carboplatin is used against some forms of cancer (mainly ovarian carcinoma, lung, head and neck cancers).
DMSO can inactivate Carboplatin's activity
Int J Mol Sci. 2022 Jun 3;23(11):6290.
Carboplatin-Induced Thrombocytopenia through JAK2 Downregulation, S-Phase Cell Cycle Arrest, and Apoptosis in Megakaryocytes
Carboplatin purchased from AbMole
Int J Oncol. 2020 Jan;56(1):47-68.
Circulating non‑coding RNA‑biomarker potential in neoadjuvant chemotherapy of triple negative breast cancer?
Carboplatin purchased from AbMole
Universitat Freiburg im Breisgau. 2020 Jul.
Investigation of chemotherapy-induced non-coding RNA expression alterations in clinical breast cancer management
Carboplatin purchased from AbMole
Cell Death Dis. 2018 Feb 12;9(2):213.
Autophagy mediates glucose starvationinduced glioblastoma cell quiescence and chemoresistance through coordinating cell metabolism, cell cycle, and survival
Carboplatin purchased from AbMole
Cell Cycle. 2018;17(10):1235-1244.
Apatinib, a novel tyrosine kinase inhibitor, suppresses tumor growth in cervical cancer and synergizes with Paclitaxel.
Carboplatin purchased from AbMole
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Source | Cell Cycle (2018 Jul). Figure 5. Carboplatin (Abmole Bioscience) |
| Method | cellular proliferation assay | |
| Cell Lines | SiHa cells | |
| Concentrations | 5 μM | |
| Incubation Time | - | |
| Results | However, we found no synergy between Apatinib and cisplatin (CI ranged from 1.149 to 5.647 in SiHa, CI ranged from 0.85 to 1.682 in Hcc94) or carboplatin (CI ranged from 0.669 to 1.402 in SiHa and from 0.955 to 1.206 in Hcc94) |
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Source | Cell Death Dis (2018). Figure 3. Carboplatin (Abmole Bioscience) |
| Method | Glucose starvation sensitizes glioblastoma cells to chemotherapies | |
| Cell Lines | U87 and U251 cells | |
| Concentrations | 50 μM | |
| Incubation Time | 5 d | |
| Results | Treatment with either temozolomide or carboplatin induced about 50 and 80% GBM cell death under normal and glucose starvation conditions, respectively |
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Source | Cell Death Dis (2018). Figure 1. Carboplatin (Abmole Bioscience) |
| Method | Glucose starvation sensitizes glioblastoma cells to chemotherapies | |
| Cell Lines | U87 and U251 cells | |
| Concentrations | 50 μM | |
| Incubation Time | 5 d | |
| Results | The cytotoxic effect was progressive and by day 5, temozolomide or carboplatin treatment caused 40–60% cell loss in U87 and U251 cells. Glucose starvation nearly doubled the cell loss to 70–90%. |
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Source | Mol Oncol (2014). Figure 4. Carboplatin |
| Method | s.c. | |
| Cell Lines | Female 6-week-old nude mice | |
| Concentrations | 100 mg/kg | |
| Incubation Time | 5 weeks | |
| Results | Both carboplatin treatment groups showed significantly reduced tumor burden as compared to the untreated controls from weeks 4 to 10 (P < 0.0001) |
| Cell Experiment | |
|---|---|
| Cell lines | 5637 cells |
| Preparation method | Cells were dosed and incubated with 290 μM carboplatin or 290 μM carboplatin combined with 0.08 μM paclitaxel for 4 h, each including 0.43 μM[14C]carboplatin (106 dpm/ml). After washing, cells were maintained with 14C carboplatin-free culture media. DNA was extracted from cells and converted to graphite and measured by AMS for 14C quantification as previously described. |
| Concentrations | 290 μM |
| Incubation time | 4 h |
| Animal Experiment | |
|---|---|
| Animal models | Female 6‐week‐old nude mice |
| Formulation | |
| Dosages | 100 mg/kg |
| Administration | s.c. |
| Molecular Weight | 371.25 |
| Formula | C6H12N2O4Pt |
| CAS Number | 41575-94-4 |
| Solubility (25°C) | Water 4 mg/mL warmed DMF 1 mg/mL (Need ultrasonic) |
| Storage | 4°C, protect from light, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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