ML385

Biological Activity

ML385 inihbits the activity of the Nrf2 transcription factor by binding to Neh1, a NC-bZIP domain that allows Nrf2 to heterodimerize with small Maf proteins, blocking NRF2 transcriptional activity. Much research has been done on activating Nrf2 because it induces cytoprotective antioxidant genes. However, some cancer cells may use Nrf2 similarly for their survival. Nrf2 is overexpressed in certain cancers such as non-small cell lung cancer (NSCLC) often due to loss of function mutations in KEAP1, which targets Nrf2 for proteasomal degradation. ML385 was found to have anti-tumor activity with specificity and selectivity for NSCLC cells with KEAP1 mutations.

Product Citations

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  J Cancer. 2025 Jan 01;16(2):417-429.

  TYMS Enhances Colorectal Cell Antioxidant Capacity Via the KEAP1-NRF2 Pathway to Resist Ferroptosis
  ML385 purchased from AbMole

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  Int J Biol Sci. 2024 Aug 07.

  The PI3K/Akt-Nrf2 Signaling Pathway and Mitophagy Synergistically Mediate Hydroxytyrosol to Alleviate Intestinal Oxidative Damage
  ML385 purchased from AbMole

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  Front. Pharmacol. 2024 Jun 28; .

  Brozopine ameliorates cognitive impairment via upregulating Nrf2, antioxidation and anti-inflammation activities
  ML385 purchased from AbMole

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  Neuromolecular Med. 2024 Oct 10;26(1):40.

  Continuous Theta Burst Stimulation Inhibits Oxidative Stress-Induced Inflammation and Autophagy in Hippocampal Neurons by Activating Glutathione Synthesis Pathway, Improving Cognitive Impairment in Sleep-Deprived Mice
  ML385 purchased from AbMole

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  J Biochem Mol Toxicol. 2024 Aug 01;38(8):e23792.

  Ezetimibe ketone protects against renal ischemia–reperfusion injury and attenuates oxidative stress via activation of the Nrf2/HO-1 signaling pathway
  ML385 purchased from AbMole

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  Neuroscience. 2024 Aug 20;559:26-38.

  The mechanism of hypoxia-inducible factor-1α enhancing the transcriptional activity of transferrin ferroportin 1 and regulating the Nrf2/HO-1 pathway in ferroptosis after cerebral ischemic injury
  ML385 purchased from AbMole

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  Nat Prod Commun. 2024 Jan.

  Pachymic Acid Alleviates Oxidative Damage of Retinal Cells Induced by Sodium Iodate or Hydrogen Peroxide by Activating the Nrf2/HO-1 Signaling Pathway
  ML385 purchased from AbMole

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  Mol Cell Toxicol. 2024 May.

  Liquiritin alleviates LPS-stimulated podocyte apoptosis and inflammation by activating Nrf2 signaling
  ML385 purchased from AbMole

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  Free Radic Biol Med. 2023 Mar 27;202:46-61.

  Mitochondrial GRIM-19 loss in parietal cells promotes spasmolytic polypeptide-expressing metaplasia through NLR family pyrin domain-containing 3 (NLRP3)-mediated IL-33 activation via a reactive oxygen species (ROS) -NRF2- Heme oxygenase-1(HO-1)–NF–кB axis
  ML385 purchased from AbMole

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  Phytomedicine. 2023 Mar 28.

  15, 16-Dihydrotanshinone I protects against ischemic stroke by inhibiting ferroptosis via the activation of nuclear factor erythroid 2-related factor 2
  ML385 purchased from AbMole

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  Int J Pharm. 2023 Sep 13;645:123389.

  Effective myocardial infarction treatment by targeted accumulation of Sulforaphane using porous magnetic silica nanoparticles
  ML385 purchased from AbMole

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  Int Immunopharmacol. 2023 May 22;120:110282.

  Melatonin attenuates lipopolysaccharide-induced immune dysfunction in dendritic cells
  ML385 purchased from AbMole

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  J Cancer Res Clin Oncol. 2023 Apr 29.

  Apatinib combined with olaparib induces ferroptosis via a p53-dependent manner in ovarian cancer
  ML385 purchased from AbMole

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  PLoS One. 2023 Sep 8;18(9):e0290264.

  Thymidylate synthase promotes esophageal squamous cell carcinoma growth by relieving oxidative stress through activating nuclear factor erythroid 2-related factor 2 expression
  ML385 purchased from AbMole

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  Mol Med Rep. 2023 Nov;28(5):202.

  Acetyl zingerone ameliorates osteoarthritis by inhibiting chondrocyte programmed cell death
  ML385 purchased from AbMole

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  Front Immunol. 2022 Feb 4;13:847861.

  Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers
  ML385 purchased from AbMole

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  Free Radic Biol Med. 2022 Nov 3;193(Pt 1):459-473.

  Sirtuin 7 mitigates renal ferroptosis, fibrosis and injury in hypertensive mice by facilitating the KLF15/Nrf2 signaling
  ML385 purchased from AbMole

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  Food Res Int. 2022 Sep 12.

  N-3 Polyunsaturated Fatty Acids Protect Esophageal Epithelial Cells from Acid Exposure
  ML385 purchased from AbMole

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  Oxid Med Cell Longev. 2022 Apr 16;2022:4505513.

  Nrf2-Mediated Ferroptosis Inhibition Exerts a Protective Effect on Acute-on-Chronic Liver Failure
  ML385 purchased from AbMole

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  Front Pharmacol. 2022 Jul 18;13:943111.

  (±)-5-bromo-2-(5-fluoro-1-hydroxyamyl) Benzoate Protects Against Oxidative Stress Injury in PC12 Cells Exposed to H 2 O 2 Through Activation of Nrf2 Pathway
  ML385 purchased from AbMole

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  Pharm Biol. 2022 Dec;60(1):638-651.

  In vivo and in vitro protective effects of shengmai injection against doxorubicin-induced cardiotoxicity
  ML385 purchased from AbMole

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  Exp Ther Med. 2022 May;23(5):358.

  Leonurine: A compound with the potential to prevent acute lung injury
  ML385 purchased from AbMole

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  Front Oncol. 2021 May 26;11:679816.

  Antidiabetic Agent DPP-4i Facilitates Murine Breast Cancer Metastasis by Oncogenic ROS-NRF2-HO-1 Axis via a Positive NRF2-HO-1 Feedback Loop
  ML385 purchased from AbMole

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  Biomed Res Int. 2021 Jun 10;2021:6616547.

  Antioral Squamous Cell Carcinoma Effects of Carvacrol via Inhibiting Inflammation, Proliferation, and Migration Related to Nrf2/Keap1 Pathway
  ML385 purchased from AbMole

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  Pharmacol Res Perspect. 2021 May;9(3):e00791.

  Dexamethasone promotes the endoplasmic reticulum stress response of bone marrow mesenchymal stem cells by activating the PERK-Nrf2 signaling pathway
  ML385 purchased from AbMole

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  Med J Chin PLA. 2021 Mar 28;Vol. 46, No. 3.

  Anti-aging effects of Schisandrin C on H2O2-induced HaCaT
  ML385 purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Front Oncol (2021). Figure 4. ML-385 (Abmole Bioscience, Houston, TX, USA)
	Method	Cell Migration and Cell Invasion Assays
	Cell Lines	Murine BC cell line 4T1 cells
	Concentrations	0, 5, 10 µM
	Incubation Time	-
	Results	We found that ML-385 treatment significantly attenuated NRF2, p-NRF2 expression (Figure 4A) and NRF2-responsive gene levels (Figure 4B) in DPP-4i-treated BC cells. Notably, DPP-4i-driven BC cell migration and invasion were significantly abrogated by ML-385 treatment with a dose-dependent manner (Figures 4C, D). Furthermore, DPP-4i–driven metastasis-associated gene levels were significantly attenuated after ML-385 treatment in BC cells (Figure 4E). These data indicate that NRF2 inhibition reverses DPP-4i–driven BC metastases in vitro.

Chemical Information

Molecular Weight	511.59
Formula	C29H25N3O4S
CAS Number	846557-71-9
Solubility (25°C)	DMSO ≥ 25 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Anju Singh, et al. ACS Chem Biol. Small Molecule Inhibitor of NRF2 Selectively Intervenes Therapeutic Resistance in KEAP1-Deficient NSCLC Tumors