CFSE (CFDA-SE)

Biological Activity

Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources. The binding of CFDA-SE fluorescent dye to cells is stable. It is not toxic and does not modify the viability of labeled cells. CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling. CFDA-SE persists in cells 24 h after labeling, allowing the long-term detection of labeled cells.

In vivo labeling of cells with CFSE is feasible with virtually no toxic effect on the labeled or adjacent cells. It has advantages in relatively long-term migration studies as demonstrated by the recovery of T-cells in the peripheral lymphoid organs.

Another CAS#: 150206-15-8

Product Citations

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  EMBO Mol Med. 2024 Mar 6;024-00051.

  The regulatory relationship between NAMPT and PD-L1 in cancer and identification of a dual-targeting inhibitor
  CFSE (CFDA-SE) purchased from AbMole

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  Neural Regen Res. 2024 May;19(5):1142-1149.

  Bromocriptine protects perilesional spinal cord neurons from lipotoxicity after spinal cord injury
  CFSE (CFDA-SE) purchased from AbMole

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  Cancer Lett. 2023 Jun 22;216278.

  Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
  CFSE (CFDA-SE) purchased from AbMole

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  Cancer Lett. 2023 Aug 1;568:216278.

  Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
  CFSE (CFDA-SE) purchased from AbMole

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  Immunology. 2023 May 19.

  EPOR activation attenuates colitis via enhancing LC3B‐dependent apoptotic cell clearance
  CFSE (CFDA-SE) purchased from AbMole

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  J Nanobiotechnology. 2022 Aug 2;20(1):356.

  Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries
  CFSE (CFDA-SE) purchased from AbMole

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  Laboratory Investigation. 2022 Jul 21.

  The N6-methyladenosine writer WTAP contributes to the induction of immune tolerance post kidney transplantation by targeting regulatory T cells
  CFSE (CFDA-SE) purchased from AbMole

Chemical Information

Molecular Weight	557.46
Formula	C29H19NO11
CAS Number	150347-59-4
Solubility (25°C)	DMSO 100 mg/mL
Storage	4°C, protect from light

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Iñigo Terrén, et al. Methods Enzymol. CFSE dilution to study human T and NK cell proliferation in vitro

[2] Jiang X, et al. Oncoimmunology. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.

[3] Stempin CC, et al. PLoS Negl Trop Dis. GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection.

[4] Edwin D Hawkins, et al. Nat Protoc. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data

[5] Ben J C Quah, et al. Nat Protoc. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

[6] Wang XQ, et al. Acta Biochim Biophys Sin (Shanghai). Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling.

[7] M E Fuller, et al. Appl Environ Microbiol. Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments

[8] Graziano M, et al. Exp Cell Res. The fate of thymocytes labeled in vivo with CFSE.