Free shipping on all orders over $ 500

MCC950

Cat. No. M6164

All AbMole products are for research use only, cannot be used for human consumption.

MCC950 Structure
Synonym:

CP-456773

Size Price Availability Quantity
5mg USD 50  USD50 In stock
10mg USD 85  USD85 In stock
50mg USD 300  USD300 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

In vitro: MCC950 (CP-456773) blocks canonical and non-canonical NLRP3 activation at nanomolar concentrations. MCC950 specifically inhibits NLRP3 but not AIM2, NLRC4 or NLRP1 activation. The effect of MCC950 on NLRP3 inflammasome activation is tested in mouse bone marrow derived macrophages (BMDM) and human monocyte derived macrophages (HMDM). The IC50 of MCC950 in BMDM is approximately 7.5 nM, while in HMDM it has a similar inhibitory capacity (IC50=8.1 nM). MCC950 also dose dependently inhibit IL-1β but not TNF-α secretion.MCC950 specifically blocks caspase-11-directed NLRP3 activation and IL-1β secretion upon stimulation of the non-canonical pathway. NLRC4-stimulated IL-1β and TNF-α secretion (as activated by Salmonella typhimurium) are not inhibited by MCC950 even at a concentration of 10 µM. MCC950 does not inhibit caspase-1 activation or IL-1β processing in response to S. typhimurium. The expression of pro-caspase-1 and pro-IL-1β in cell lysates is not substantially affected by MCC950 treatment.

In vivo: MCC950 (CP-456773) reduces Interleukin-1p (IL-1β) production and attenuates the severity of experimental autoimmune encephalomyelitis (EAE), a disease model of multiple sclerosis. Pre-treatment with MCC950 reduces serum concentrations of IL-1β and IL-6 while it does not considerably decrease the amount of TNF-α. Treatment of mice with MCC950 delays the onset and reduced the severity of EAE. Intracellular cytokine staining and FACS analysis of brain mononuclear cells from mice sacrificed on day 22 shows modestly reduced frequencies of IL-17 and IFN-γ producing CD3+ T cells in MCC950 treated mice in comparison with PBS-treated mice. IFN-γ and particularly IL-17 producing cell numbers are also reduced in both the CD4+ and γδ+ sub-populations of CD3+ T cells.

Product Citations
Customer Product Validations & Biological Datas
Source Cell Reports 2020 May. Figure 3. MCC950 (Abmole Bioscience, USA)
Method C. trachomatis infection
Cell Lines Human primary monocyte-derived macrophages
Concentrations 300 nM
Incubation Time 1 h
Results MCC950 treatment largely abolished infection-induced IL-1b secretion and pyroptosis.
Protocol (for reference only)
Cell Experiment
Cell lines BMDM cell
Preparation method For non-canonical inflammasome activation cells are primed with 100 ng/mL Pam3CSK4 for 4 h, medium is removed and replaced with SFM containing DMSO or MCC950 and 2 µg/mL LPS is transfected using 0.25% FuGENE for 16 h. Supernatants are removed and analysed using ELISA kits. LDH release is measured using the CytoTox96 non-radioactive cytotoxicity assay.
Concentrations 0.001-10 µM
Incubation time 16 h
Animal Experiment
Animal models C57BL/6 mice
Formulation dissolved in DMSO and diluted with PBS
Dosages 10 mg/kg
Administration i.p.
Chemical Information
Molecular Weight 404.48
Formula C20H24N2O5S
CAS Number 210826-40-7
Solubility (25°C) 28 mg/mL in DMSO
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Cong-Fa Huang, et al. J Exp Clin Cancer Res. NLRP3 inflammasome activation promotes inflammation-induced carcinogenesis in head and neck squamous cell carcinoma

[2] Rebecca C Coll, et al. Nat Med. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases

Related Pyroptosis Products
INF 195 

INF 195 is an NLRP3 inhibitor.

Azalamellarin N

Azalamellarin N is an inhibitor of pyroptosis and has different inhibitory effects on different pyroptosis inducers. Azalamellarin N inhibits pyroptosis by targeting molecules that act upstream of NLRP3 inflammasome activation, rather than directly targeting components of the NLRP3 inflammasome.

Sodium metatungstate

Sodium metatungstate (Sodium polyoxotungstate) is a potent ecto-nucleoside triphosphate diphosphohydrolase (ENTPDase) inhibitor, with Ki values of 2.58 μM, 3.26 μM, and 28.8 μM for NTPDase 1 (CD39), NTPDase 3 and NTPDase 2 respectively.

LDC7559

LDC7559 is a gasdermin D (GSDMD) inhibitor via blocking neutrophil extracellular trap (NET) in the late stages .

CY-09

CY-09 is a selective direct inhibitor of NLRP3 with anti-inflammatory and antidepressant activity, which inhibits inflammatory cytokine production and TRPA1 activation by inhibiting the activation of the NLRP3 inflammatory vesicle, thereby decreasing the intracellular Ca2+ level and reducing the pro-inflammatory polarization of macrophages, alleviating pain and injury in animals. In addition, CY-09 can inhibit pyroptosis.

  Catalog
Abmole Inhibitor Catalog




Keywords: MCC950, CP-456773 supplier, Pyroptosis, inhibitors, activators

All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.



Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.