DTT

Biological Activity

Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent; as a reducing or "deprotecting" agent for thiolated DNA.

Product Citations

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  Nucleic Acids Res . 2022 Aug 12;50(14):7938-7958.

  HDAC1 and PRC2 mediate combinatorial control in SPI1/PU.1-dependent gene repression in murine erythroleukaemia
  DTT purchased from AbMole

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  Nat Commun. 2020 Dec 22;11(1):6438.

  MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression
  DTT purchased from AbMole

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  Exp Cell Res. 2020 Nov 1;396(1):112237.

  LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway
  DTT purchased from AbMole

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  J Virol. 2020 Feb 14;94(5):e01791-19.

  Targeting Kaposi's Sarcoma-Associated Herpesvirus ORF21 Tyrosine Kinase and Viral Lytic Reactivation by Tyrosine Kinase Inhibitors Approved for Clinical Use
  DTT purchased from AbMole

Chemical Information

Molecular Weight	154.25
Formula	C4H10O2S2
CAS Number	3483-12-3
Solubility (25°C)	DMSO: ≥ 75 mg/mL Water: ≥ 90 mg/mL
Storage	2-8°C, dry, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Amber L Kramer, et al. Assessing the oxidative potential of PAHs in ambient PM 2.5 using the DTT consumption assay

[2] Abdul Hadi Mehmood, et al. The development of an endoplasmic reticulum-targeting fluorescent probe for the imaging of 1,4-dithiothreitol (DTT) in living cells

[3] Yiwen Wang, et al. Toxicity of Dithiothreitol (DTT) to Drosophila melanogaster

[4] Brittany C Hudson, et al. The effects of dithiothreitol (DTT) on fluorescent qPCR dyes

[5] Heng Zhou, et al. Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals