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Avidin (from egg white)

Cat. No. M22515
Avidin (from egg white) Structure

Immunopure Avidin

Size Price Availability Quantity
5mg USD 220  USD220 In stock
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Quality Control & Documentation
  • Purity: ≥98%, 10-15 units/mg protein (E1%/280)
  • COA
  • MSDS
Biological Activity

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Chemical Information
CAS Number 1405-69-2
Storage -20°C
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Vesa P Hytnen. Methods Enzymol. (Strept)avidin as a template for ligands other than biotin: An overview

[2] Akshay Jain, et al. J Control Release. The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

[3] Hanna P Lesch, et al. Expert Opin Drug Deliv. Avidin-biotin technology in targeted therapy

[4] F M Richards. Methods Enzymol. Avidin-biotin technology. Reflections

[5] N M Green. Adv Protein Chem. Avidin

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Keywords: Avidin (from egg white), Immunopure Avidin supplier, Western Blot, inhibitors, activators

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