Sulforaphane

Biological Activity

Sulforaphane inhibits the lipopolysaccharide LPS induced HDAC6, HDAC10 and DNA methyltransferase (DNMT3a) gene expression, whereas up-regulated the expression of DNMT1 gene.Sulforaphane inhibits the global HDAC activity, and suppressed moDCs differentiation from immature to mature DCs through down-regulating the CD40, CD80 and CD86 expression and led further to enhanced phagocytosis of moDCs.Sulforaphane showed a protective role in LPS induced cell apoptosis through suppressing the IRF6 and TGF-ß1 production. Sulforaphane impaired the pro-inflammatory cytokine TNF-α and IL-1ß secretion into the cell culture supernatants that were induced in moDCs by LPS stimulation, whereas Sulforaphane increased the cellular-resident TNF-α accumulation.Sulforaphane plays a protective role against APAP-mediated hepatotoxicity through antioxidant effects mediated by HO-1 induction. Sulforaphane has preventive action in oxidative stress-mediated liver injury.Sulforaphane has the potential to improve cognitive function in patients with schizophrenia.Sulforaphane protected cells against glycative damage by inhibiting the activation of caspase-3 enzyme, reducing the phosphorylation of MAPK signaling pathways (ERK1/2, JNK, and p38), reducing oxidative stress and increasing intracellular GSH levels.

Product Citations

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  Biochem Biophys Res Commun. 2022 Mar 2.

  Sulforaphane protects myocardium from ischemia-reperfusion injury by regulating CaMKIIN2 and CaMKIIδ
  Sulforaphane purchased from AbMole

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  Food Funct. 2021 Nov 1;12(21):10950-10966.

  Physalin B inhibits PDGF-BB-induced VSMC proliferation, migration and phenotypic transformation by activating the Nrf2 pathway
  Sulforaphane purchased from AbMole

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  Sci Total Environ. 2021 Oct 1;789:148029.

  The aggravation of allergic airway inflammation with dibutyl phthalate involved in Nrf2-mediated activation of the mast cells
  Sulforaphane purchased from AbMole

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  Environ Pollut. 2020 Apr;259:113812.

  Sulforaphane prevents chromium-induced lung injury in rats via activation of the Akt/GSK-3β/Fyn pathway.
  Sulforaphane purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Sci Total Environ (2021). Figure 3. SFN (Abmole Bioscience Inc, Houston, TX, USA)
	Method	injected
	Cell Lines	male Wistar rats
	Concentrations	4 mg/kg
	Incubation Time	63 days
	Results	Notably, the OVA-sIgE level and the LDH activity in SFN-administered rats were lower than those in the DBP high-dose group

	Source	Sci Total Environ (2021). Figure 2. SFN (Abmole Bioscience Inc, Houston, TX, USA)
	Method	injected
	Cell Lines	male Wistar rats
	Concentrations	4 mg/kg
	Incubation Time	63 days
	Results	Cell counts in BALF were increased through DBP exposure compared with the OVA group. However, the increase of cell counts in BALF was notably decreased by the administration of SFN compared to the DBP high-dose group

Chemical Information

Molecular Weight	177.29
Formula	C6H11NOS2
CAS Number	4478-93-7
Solubility (25°C)	DMSO 50 mg/mL Ethanol
Storage	-20°C, dry, sealed, protect from light

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Angeloni C, et al. Chem Res Toxicol. Neuroprotective effect of sulforaphane against methylglyoxal cytotoxicity.

[2] Oguz A, et al. Int J Surg. The effects of sulforaphane on the liver and remote organ damage in hepatic ischemia-reperfusion model formed with pringle maneuver in rats.

[3] Shiina A, et al. Clin Psychopharmacol Neurosci. An Open Study of Sulforaphane-rich Broccoli Sprout Extract in Patients with Schizophrenia.

[4] Noh JR, et al. Food Chem Toxicol. Sulforaphane protects against acetaminophen-induced hepatotoxicity.

[5] Qu X, et al. PLoS One. Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide.