MTT is a reagent used in the measurement of cell proliferation.
Biomed Pharmacother. 2023 May;161:114575.
Targeting the TNF-α–TNFR interaction with EGCG to block NF-κB signaling in human synovial fibroblasts
MTT purchased from AbMole
Biosci Rep. 2020 Jul 31;40(7):BSR20200730.
NLRP3 augmented resistance to gemcitabine in triple-negative breast cancer cells via EMT/IL-1β/Wnt/β-catenin signaling pathway
MTT purchased from AbMole
Drug Discov Ther. 2020 Sep 8;14(4):197-203.
MicroRNA analysis of NCI-60 human cancer cells indicates that miR-720 and miR-887 are potential therapeutic biomarkers for breast cancer
MTT purchased from AbMole
J Steroid Biochem Mol Biol. 2013 Nov;138:54-62.
Effect of androgens on different breast cancer cells co-cultured with or without breast adipose fibroblasts
MTT purchased from AbMole
| Molecular Weight | 414.32 |
| Formula | C18H16N5SBr |
| CAS Number | 298-93-1 |
| Solubility (25°C) | Water 4.2 mg/mL |
| Storage | 2-8°C, dry, protect from light, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Priti Kumar, et al. Cold Spring Harb Protoc . Analysis of Cell Viability by the MTT Assay
[3] Johan van Meerloo, et al. Methods Mol Biol . Cell sensitivity assays: the MTT assay
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AbMole Antifade Mounting Medium with DAPI is a ready-to-use solution that can not only slow down the quenching of fluorescent dyes, but also complete the DAPI staining of cell nuclei while mounting the slides. |
| Antifade Mounting Medium
AbMole Antifade Mounting Medium is a sealing reagent used to slow the fluorescence quenching. It can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect. |
| WST-1
WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm. |
| XTT sodium salt
XTT sodium salt is a tetrazolium salt, which forms water-soluble formazan on bioreduction. |
| Cell Counting Kit-8
Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. |
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