Mitomycin C

Biological Activity

Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis.

Product Citations

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  Skin Res Technol. 2023 Feb;29(2):e13290.

  The use of human‐derived feeder layers for the cultivation of transplantable human epidermal cell sheet to repair second degree burn wounds
  Mitomycin C purchased from AbMole

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  Comput Math Methods Med. 2022 Jul 13;2022:2517463.

  MicroRNA-424-5p Alleviates Isoflurane Anesthesia-Induced Neurotoxicity in Human Embryonic Stem Cell-Derived Neurons by Targeting FASN
  Mitomycin C purchased from AbMole

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  Biomed Res Int. 2021 Jun 10;2021:6616547.

  Antioral Squamous Cell Carcinoma Effects of Carvacrol via Inhibiting Inflammation, Proliferation, and Migration Related to Nrf2/Keap1 Pathway
  Mitomycin C purchased from AbMole

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  Int J Mol Sci. 2020 Apr 30;21(9):3164.

  Leukamenin E Induces K8/18 Phosphorylation and Blocks the Assembly of Keratin Filament Networks Through ERK Activation
  Mitomycin C purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Int J Mol Sci. 2020 Apr. Figure 9. Mitomycin C (Abmole Bioscience, USA)
	Method	Wound healing assays
	Cell Lines	human pancreatic cancer cells (Panc-1)
	Concentrations	10 mg/mL
	Incubation Time	12 h
	Results	Leukamenin E induced an inhibitory e ect on the migration of HUVECs and Panc-1 cells using both transwell and wound healing assay.

Chemical Information

Molecular Weight	334.37
Formula	C15H18N4O5
CAS Number	50-07-7
Solubility (25°C)	DMSO 60 mg/mL
Storage	-20°C, protect from light, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Michelle Vang Mouritzen, et al. J Vis Exp . Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

[2] Laura Dittmar, et al. Hum Immunol. Immunosuppressive properties of mitomycin C-incubated human myeloid blood cells (MIC) in vitro

[3] Rustagi T, et al. J Clin Gastroenterol. Treatment of Refractory Gastrointestinal Strictures With Mitomycin C: A Systematic Review.

[4] Antonio Di Grazia, et al. Antimicrob Agents Chemother . Temporins A and B stimulate migration of HaCaT keratinocytes and kill intracellular Staphylococcus aureus

[5] Christian Andreas Radu, et al. J Surg Res. Mitomycin-C-treated peripheral blood mononuclear cells (PBMCs) prolong allograft survival in composite tissue allotransplantation

[6] P L Leenaerts, et al. Transplantation. Evidence that stimulator cell-derived IL-6 and IL-1 are released in the mixed lymphocyte culture but are not requisite for responder T cell proliferation