All AbMole products are for research use only, cannot be used for human consumption.
Cyclosporine A (CsA，Cyclosporin A) is an immunosuppressant that binds to cyclophilin and inhibits calcineurin with an IC50 of 7 nM. Cyclosporine A [CsA] binds to the cyclophilin 18 (Cyp18)-CsA complex. the Cyp18-CsA complex recruits calcium-regulated neurophosphatase (CaN), leading to the blocking of cytokine gene transcription in activated T cells. CsA is a highly specific inhibitor of T-cell activation.
Mol Pharm. 2024 Apr 26.
STING-Targeted PET Imaging for Specific Detection and Therapeutic Monitoring of Myocarditis
Cyclosporine A purchased from AbMole
Cardiology Discovery. 2024 Nov 15.
Minimally Invasive Transthoracic Intramyocardial Cellular Transplantation Under Echocardiographic Guidance for Myocardial Impairment
Cyclosporine A purchased from AbMole
Stem Cell Rev Rep. 2022 Jun 20.
Promoting Oligodendrocyte Differentiation from Human Induced Pluripotent Stem Cells by Activating Endocannabinoid Signaling for Treating Spinal Cord Injury
Cyclosporine A purchased from AbMole
J Ethnopharmacol. 2022 Sep 15;295:115409.
Senegenin alleviates Aβ1-42 induced cell damage through triggering mitophagy
Cyclosporine A purchased from AbMole
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Source | Can J Physiol Pharmacol (2014). Figure 2. Cyclosporine A |
| Method | MTT assay | |
| Cell Lines | NRK-52E and LLC-PK1 cells | |
| Concentrations | 10 mg/L | |
| Incubation Time | 24 and 48 h | |
| Results | A noticeably higher susceptibility of the rat kidney cell line to toxicity in the presence of CyA in vehicle was noted compared with the pig kidney cell line |
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Source | Can J Physiol Pharmacol (2014). Figure 1. Cyclosporine A |
| Method | Hepatocytes | |
| Cell Lines | NL and HL rat | |
| Concentrations | 2.5 mg/L | |
| Incubation Time | 5 and 30 min | |
| Results | In the hepatocytes exposed to CyA–2% NL rat serum, there was an initial spike in the uptake of CyA by the cells regardless of pretreatment |
| Cell Experiment | |
|---|---|
| Cell lines | Jurkat cells (clone J77) |
| Preparation method | Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments. Cells (106) are suspended in 1 ml of complete medium in microcentrifuge tubes; 1 μl of ethanol or of the ethanolic solution of Cyclosporin A is added, and the cells are incubated at 37°C for 1 hr. Cells are washed twice with 1 ml of PBS on ice and lysed in 50μl of hypotonic buffer containing 50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM dithiothreitol; and 50 μg of phenylmethylsulfonyl fluoride, 50 μg of soybean trypsin inhibitor, 5 μg of leupeptin, and 5 μg of aprotinin per ml. Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30°C and then are centrifuged at 4°C for 10 min at 12,000×g. |
| Concentrations | ~100 nM |
| Incubation time | 1 h |
| Animal Experiment | |
|---|---|
| Animal models | Rat |
| Formulation | 0.5% solution of tragacanth |
| Dosages | 45 mg/kg |
| Administration | oral |
| Molecular Weight | 1202.61 |
| Formula | C62H111N11O12 |
| CAS Number | 59865-13-3 |
| Solubility (25°C) | DMSO ≥ 45 mg/mL |
| Storage | 2-8°C, dry, sealed, protect from light |
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All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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