All AbMole products are for research use only, cannot be used for human consumption.
IWP-2 is an inhibitor of Wnt production. IWP-2 inactivates Porcupine, a membrane-bound O-acyltransferase responsible for palmitoylating Wnt proteins, which is essential for their signaling ability and secretion. IWP-2 selectively inhibits palmitoylation of Wnt, and blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. IWP-2 is useful in both suppressing embryonic stem cell self-renewal and decreasing cancer cell proliferation, migration and invasion.
Cell Death Dis. 2024 Mar 26;15(3):234.
Nuclear receptor NURR1 functions to promote stemness and epithelial-mesenchymal transition in prostate cancer via its targeting of Wnt/β-catenin signaling pathway
IWP-2 purchased from AbMole
Chem Biol Interact. 2023 Dec 1;386:110777.
Assessment of doxorubicin toxicity using human cardiac organoids: A novel model for evaluating drug cardiotoxicity
IWP-2 purchased from AbMole
Stem Cell Reports. 2021 Jun 8;16(6):1568-1583.
Photobiomodulation therapy for hair regeneration: A synergetic activation of β-CATENIN in hair follicle stem cells by ROS and paracrine WNTs
IWP-2 purchased from AbMole
Oncol Rep. 2020 Aug;44(2):577-588.
TNF‑α promotes the malignant transformation of intestinal stem cells through the NF‑κB and Wnt/β‑catenin signaling pathways
IWP-2 purchased from AbMole
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Source | ONCOLOGY REPORTS (2020 Aug). Figure 6. IWP-2 (Abmole Bioscience, US) |
| Method | western blot | |
| Cell Lines | NCM460s cells | |
| Concentrations | 1, 10, 100 µM | |
| Incubation Time | 3 h | |
| Results | Notably, IWP-2 also counteracted TNF-α-induced activation of the NF-κB pathway in NCM460s cells, including increased phosphorylation of IKKα/β and IκBα, decreased protein levels of IκBα and nuclear translocation of p65 |
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Source | Front Immunol (2018). Figure 6. IWP-2 |
| Method | injected intravenously | |
| Cell Lines | C57BL/6 mice | |
| Concentrations | 20 mg/kg | |
| Incubation Time | 4.5 h | |
| Results | This view is supported by reduced percentages of IFN-γ+ liver CD3+NK1.1+ cells in mice treated with IWP-2, a small molecule inhibitor of the acyltransferase Porcupine, which plays a central role in Wnt secretion |
| Cell Experiment | |
|---|---|
| Cell lines | MKN28 cell line |
| Preparation method | Cell proliferation assay CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay kit was used. Transwell migration and invasion assay Haptotaxis chambers (8-μm pore size) without or with 5 mg/ml matrigel were used for migration and invasion assays, respectively. The experiments were repeated three times independently. |
| Concentrations | 0,5,10,20,50µM |
| Incubation time | 5 days |
| Animal Experiment | |
|---|---|
| Animal models | six month-old zebrafish model |
| Formulation | 0.1% DMSO |
| Dosages | 10 μM |
| Administration | in aquarium water supplemented with 10 μM IWR-1 or in 0.1% DMSO as a control |
| Molecular Weight | 466.6 |
| Formula | C22H18N4O2S3 |
| CAS Number | 686770-61-6 |
| Solubility (25°C) | DMSO 2 mg/mL DMF 8 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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Products are for research use only. Not for human use. We do not sell to patients.
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