Cycloheximide

Biological Activity

Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome) thus blocking translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.

Product Citations

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  Adv Sci (Weinh). 2024 Jan;e2207435.

  Defective Homologous Recombination Repair By Up-Regulating Lnc-HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage
  Cycloheximide purchased from AbMole

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  Autophagy. 2024 Jun 6.

  The novel lnc-HZ12 suppresses autophagy degradation of BBC3 by preventing its interactions with HSPA8 to induce trophoblast cell apoptosis
  Cycloheximide purchased from AbMole

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  Cancer Lett. 2024 Apr 6;590:216861.

  TRAF6 enhances PD-L1 expression through YAP1-TFCP2 signaling in melanoma
  Cycloheximide purchased from AbMole

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  iScience. 2024 May 15.

  Deficiency in Prader-Willi syndrome gene necdin leads to attenuated cardiac contractility
  Cycloheximide purchased from AbMole

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  J Biol Chem. 2024 Mar 25;300(5):107226.

  Epstein-Barr virus suppresses N6-Methyladenosine modification of TLR9 to promote immune evasion
  Cycloheximide purchased from AbMole

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  Cell Signal. 2024 May 26;111235.

  BMSC derived EVs inhibit colorectal Cancer progression by transporting MAGI2-AS3 or something similar
  Cycloheximide purchased from AbMole

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  Mol Carcinog. 2024 Jan.

  SN-38, an active metabolite of irinotecan, inhibits transcription of nuclear factor erythroid 2-related factor 2 and enhances drug sensitivity of colorectal cancer cells
  Cycloheximide purchased from AbMole

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  iScience. 2024 Jan 19.

  FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-β pathway via facilitating CD109 expression
  Cycloheximide purchased from AbMole

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  Mol Cell Biochem. 2024 Feb 17.

  Tripartite-motif 3 represses ovarian cancer progression by downregulating lactate dehydrogenase A and inhibiting AKT signaling
  Cycloheximide purchased from AbMole

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  J Transl Med. 2023 May 5;21(1):303.

  SYTL2 promotes metastasis of prostate cancer cells by enhancing FSCN1-mediated pseudopodia formation and invasion
  Cycloheximide purchased from AbMole

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  Cell Death Discov. 2023 Apr 26.

  Ammonium Tetrathiomolybdate Relieves Oxidative Stress in Cisplatin-induced Acute Kidney Injury via NRF2 Signaling Pathway
  Cycloheximide purchased from AbMole

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  Cell Death Discov. 2023 Jul 25;9(1):259.

  Ammonium tetrathiomolybdate relieves oxidative stress in cisplatin-induced acute kidney injury via NRF2 signaling pathway
  Cycloheximide purchased from AbMole

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  J Cell Mol Med. 2023 Aug 29.

  Alleviation of temporomandibular joint osteoarthritis by targeting RIPK1-mediated inflammatory signalling
  Cycloheximide purchased from AbMole

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  FASEB J. 2023 Oct;37(10):e23183.

  IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6A-dependent manner
  Cycloheximide purchased from AbMole

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  New Journal of Chemistry. 2023 Jun 20.

  Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection
  Cycloheximide purchased from AbMole

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  New J. Chem. 2023 Jun 28;47, 14344-14354.

  Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection
  Cycloheximide purchased from AbMole

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  Biochem Genet. 2023 Nov 11.

  Functional Characterization of a Novel SLC4A4 Variant and Uniparental Isodisomy in Proximal Renal Tubular Acidosis Patient
  Cycloheximide purchased from AbMole

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  Cell Death Differ. 2022 Jul;29(7):1395-1408.

  SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK
  Cycloheximide purchased from AbMole

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  Research Square. 2022 Oct 12.

  IGF2BP2 promotes ovarian cancer growth and metastasis via upregulating CKAP2L protein expression in a m6A-dependent manner
  Cycloheximide purchased from AbMole

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  Ecotoxicol Environ Saf. 2022 Jun 1;237:113564.

  Environmental BPDE induced human trophoblast cell apoptosis by up-regulating lnc-HZ01/p53 positive feedback loop
  Cycloheximide purchased from AbMole

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  Virol Sin. 2022 Aug 16;S1995-820X(22)00143-2.

  CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells
  Cycloheximide purchased from AbMole

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  Cell Biol Toxicol. 2022 Apr;38(2):291-310.

  Lnc-HZ08 regulates BPDE-induced trophoblast cell dysfunctions by promoting PI3K ubiquitin degradation and is associated with miscarriage
  Cycloheximide purchased from AbMole

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  Bioengineered. 2022 Apr;13(4):8000-8012.

  UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization
  Cycloheximide purchased from AbMole

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  J Biol Chem. 2022 Nov 21;102734.

  Ubiquitin-specific peptidase 14 maintains estrogen receptor α stability via its deubiquitination activity in endometrial cancer
  Cycloheximide purchased from AbMole

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  Mol Cell Endocrinol. 2022 Apr 5;545:111575.

  An intron mutation of HNF1A causes abnormal splicing and impairs its activity as a transcription factor
  Cycloheximide purchased from AbMole

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  Adv Sci (Weinh). 2021 Feb.

  Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple‐Negative Breast Cancer Tumor Growth
  Cycloheximide purchased from AbMole

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  Adv Sci (Weinh). 2021 Jan 6;8(4):2003205.

  Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple-Negative Breast Cancer Tumor Growth
  Cycloheximide purchased from AbMole

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  Clin Transl Med. 2021 Apr;11(4):e395.

  FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression
  Cycloheximide purchased from AbMole

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  Int J Mol Sci. 2021 Aug 16;22(16):8779.

  Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency
  Cycloheximide purchased from AbMole

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  J Cancer. 2021 Oct 17;12(23):7041-7051.

  N6-methyladenosine regulates ATM expression and downstream signaling
  Cycloheximide purchased from AbMole

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  Cell Rep. 2020 Jul 28;32(4):107974.

  L ARP7 Is a BRCA1 Ubiquitinase Substrate and Regulates Genome Stability and Tumorigenesis
  Cycloheximide purchased from AbMole

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  Cell Rep. 2020 Jul 28;32(4):107974.

  LARP7 is a BRCA1 ubiquitinase substrate and regulates genome stability and tumorigenesis
  Cycloheximide purchased from AbMole

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  Theranostics. 2020 Apr 27;10(13):5671-5686.

  SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma
  Cycloheximide purchased from AbMole

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  Cell Oncol (Dordr). 2020 Oct 19.

  Ubiquitin-specific protease 2a promotes hepatocellular carcinoma progression via deubiquitination and stabilization of RAB1A
  Cycloheximide purchased from AbMole

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  Cell Microbiol. 2020 Mar;22(3):e13148.

  Hepatitis B virus X protein modulates upregulation of DHX9 to promote viral DNA replication.
  Cycloheximide purchased from AbMole

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  Life Sci. 2020 Sep 15;257:118021.

  TRIB3 destabilizes tumor suppressor PPARα expression through ubiquitin-mediated proteasome degradation in acute myeloid leukemia
  Cycloheximide purchased from AbMole

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  Front Cell Infect Microbiol. 2020 Apr 16;10:158.

  Difluoromethylornithine, a Decarboxylase 1 Inhibitor, Suppresses Hepatitis B Virus Replication by Reducing HBc Protein Levels
  Cycloheximide purchased from AbMole

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  Aging (Albany NY). 2018 Oct 18;10(10):2783-2799.

  Ubiquitin-specific protease 4 promotes metastasis of hepatocellular carcinoma by increasing TGF-β signaling-induced epithelial-mesenchymal transition.
  Cycloheximide purchased from AbMole

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  Biotechnol Lett. 2018 Aug;40:1181-1188.

  UHRF2 promotes DNA damage response by decreasing p21 via RING finger domain.
  Cycloheximide purchased from AbMole

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  Protein Cell. 2017 Mar;8(3):202-218.

  E3 ligase UHRF2 stabilizes the acetyltransferase TIP60 and regulates H3K9ac and H3K14ac via RING finger domain
  Cycloheximide purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Biotechnol Lett (2018 Aug). Figure 3. cycloheximide (Abmole Bioscience)
	Method	Western blotting
	Cell Lines	Human embryonic kidney 293 cells
	Concentrations	50 ug/mL
	Incubation Time	3 h
	Results	The overexpression of UHRF2 shortened the half-life of p21 to 1 h compared to 3 h under controlled conditions.

	Source	Protein and Cell (2016). Figure 4.CHX (ABmole Bioscience, USA)
	Method	Half-life experiments
	Cell Lines	Human normal liver cells (LO2)
	Concentrations	100 μg/mL
	Incubation Time
	Results	Graph represents collated results rom three independent half-life experiments performed in HEK293, LO2 and HepG2 cells. TIP60 levels were normalized to 1 at time zero.

Chemical Information

Molecular Weight	281.35
Formula	C15H23NO4
CAS Number	66-81-9
Solubility (25°C)	DMSO ≥ 80 mg/mL Water 15 mg/mL (ultrasonic and warming)
Storage	4°C, protect from light

References

[1] Teklebirhan G,et.al. Int J Microbiol. Prevalence of Dermatophytic Infection and the Spectrum of Dermatophytes in Patients Attending a Tertiary Hospital in Addis Ababa, Ethiopia.