Acetylcysteine (NAC)

Biological Activity

Acetylcysteine(NAC; N-acetyl-l-cysteine) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor.

Product Citations

• 

  Environ Toxicol. 2022 Oct;37(10):2552-2565.

  Polystyrene nanoplastics exacerbated lipopolysaccharide‐induced necroptosis and inflammation via the ROS/MAPK pathway in mice spleen
  Acetylcysteine (NAC) purchased from AbMole

• 

  Neurochem Res. 2022 Oct 30.

  MiR-29c Inhibits TNF-α-Induced ROS Production and Apoptosis in Mouse Hippocampal HT22 Cell Line
  Acetylcysteine (NAC) purchased from AbMole

• 

  J Pharm Anal. 2022 Aug;12(4):570-582.

  18beta-Glycyrrhetinic acid induces ROS-mediated apoptosis to ameliorate hepatic fibrosis by targeting PRDX1/2 in activated HSCs
  Acetylcysteine (NAC) purchased from AbMole

• 

  J Hazard Mater. 2022 Oct 5;439:129502.

  Polystyrene nanoparticle exposure supports ROS-NLRP3 axis-dependent DNA-NET to promote liver inflammation
  Acetylcysteine (NAC) purchased from AbMole

• 

  Acta Pharm Sin B. 2022 May;12(5):2300-2314.

  Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1
  Acetylcysteine (NAC) purchased from AbMole

• 

  Acta Pharmaceutica Sinica B. 2021 Nov 10.

  Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1
  Acetylcysteine (NAC) purchased from AbMole

• 

  Ecotoxicol Environ Saf. 2021 Oct 1;222:112488.

  H2S exposure induces cell death in the broiler thymus via the ROS-initiated JNK/MST1/FOXO1 pathway
  Acetylcysteine (NAC) purchased from AbMole

• 

  J Infect Dis. 2021 Oct 28;224(8):1333-1344.

  Distinct Mitochondria-Mediated T-Cell Apoptosis Responses in Children and Adults With Coronavirus Disease 2019
  Acetylcysteine (NAC) purchased from AbMole

• 

  Front Oncol. 2021 Sep 24;11:728047.

  Antidiabetic DPP-4 Inhibitors Reprogram Tumor Microenvironment That Facilitates Murine Breast Cancer Metastasis Through Interaction With Cancer Cells via a ROS–NF-кB–NLRP3 Axis
  Acetylcysteine (NAC) purchased from AbMole

• 

  Front Oncol. 2021 May 26;11:679816.

  Antidiabetic Agent DPP-4i Facilitates Murine Breast Cancer Metastasis by Oncogenic ROS-NRF2-HO-1 Axis via a Positive NRF2-HO-1 Feedback Loop
  Acetylcysteine (NAC) purchased from AbMole

• 

  Ecotoxicol Environ Saf. 2021 Oct 1;222:112488.

  H 2 S exposure induces cell death in the broiler thymus via the ROS-initiated JNK/MST1/FOXO1 pathway
  Acetylcysteine (NAC) purchased from AbMole

• 

  Redox Biol. 2021 Aug;44:102003.

  Roles of selenoprotein S in reactive oxygen species-dependent neutrophil extracellular trap formation induced by selenium-deficient arteritis
  Acetylcysteine (NAC) purchased from AbMole

• 

  Pathogens. 2020 Aug 23;9(9):693.

  Giardia duodenalis Induces Apoptosis in Intestinal Epithelial Cells via Reactive Oxygen Species-Mediated Mitochondrial Pathway In Vitro
  Acetylcysteine (NAC) purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Pathogens 2020 Aug. Figure 5. N-acetyl-l-cysteine (Abmole Bioscience Inc, Houston, TX, USA)
	Method	Cell Viability Assay
	Cell Lines	Caco-2 cells
	Concentrations	10 mM/well
	Incubation Time	6 h
	Results	It was shown in Figure 5A that pre-incubation of Caco-2 cells with NAC for 3 h before exposure to trophozoites could block the release of Cyt-c. The activation of CASP3 was diminished by NAC in trophozoites-treated cells

Chemical Information

Molecular Weight	163.19
Formula	C5H9NO3S
CAS Number	616-91-1
Solubility (25°C)	DMSO 30 mg/mL Water 30 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Qiuting Zhang, et al. J Cancer. Artemisinin Derivatives Inhibit Non-small Cell Lung Cancer Cells Through Induction of ROS-dependent Apoptosis/Ferroptosis

[2] Stutzman D, et al. Am J Health Syst Pharm. Acetylcysteine for treatment of autism spectrum disorder symptoms.