Lipopolysaccharides

Biological Activity

Lipopolysaccharides (LPS) is an endotoxin derived from the outer leaflet of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is a highly immunogenic antigen which can enhance immune responses. This product is derived from Escherichia coli O55:B5. Its potency is more than 500,000 EU/mg.

Product Citations

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  Mol Med Rep. 2022 Jul;26(1):220.

  Tranilast attenuates lipopolysaccharide‑induced lung injury via the CXCR4/JAK2/STAT3 signaling pathway
  Lipopolysaccharides purchased from AbMole

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  Front Immunol. 2022 Aug 15;13:914381.

  YAP9/A20 complex suppresses proinflammatory responses and provides novel anti-inflammatory therapeutic potentials
  Lipopolysaccharides purchased from AbMole

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  J Funct Foods. 2022 Aug;Volume 95, 105191.

  Royal jelly attenuates LPS-induced immune dysfunction of dendritic cells via Nrf2/HO-1 axis
  Lipopolysaccharides purchased from AbMole

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  Can Respir J. 2022 Jul 31;2022:8433960.

  Dexmedetomidine Attenuates LPS-Stimulated Alveolar Type II Cells' Injury through Upregulation of miR-140-3p and Partial Suppression of PD-L1 Involving Inactivating JNK-Bnip3 Pathway
  Lipopolysaccharides purchased from AbMole

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  Oxid Med Cell Longev. 2022 Apr 16;2022:4505513.

  Nrf2-Mediated Ferroptosis Inhibition Exerts a Protective Effect on Acute-on-Chronic Liver Failure
  Lipopolysaccharides purchased from AbMole

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  Mar Drυgs. 2020 Oct 28;18(11):538.

  Immunostimulatory Effects of Polysaccharides from Spirulina platensis In Vivo and Vitro and Their Activation Mechanism on RAW246.7 Macrophages
  Lipopolysaccharides purchased from AbMole

Chemical Information

Solubility (25°C)	Water ≥ 5 mg/mL
Storage	2-8°C, dry, protect from light, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Hai-Ping Zhu, et al. Mol Med Rep. Regulatory mechanism of NOV/CCN3 in the inflammation and apoptosis of lung epithelial alveolar cells upon lipopolysaccharide stimulation

[2] Lei Yang, et al. Am J Transl Res. Resveratrol alleviates sepsis-induced acute lung injury by suppressing inflammation and apoptosis of alveolar macrophage cells

[3] H T L Lai, et al. Poult Sci. Effects of Repeated Intratracheally Administered Lipopolysaccharide on Primary and Secondary Specific Antibody Responses and on Body Weight Gain of Broilers.

[4] D S Kabanov, et al. Biochemistry (Mosc). Structural Analysis of Lipopolysaccharides From Gram-negative Bacteria.