All AbMole products are for research use only, cannot be used for human consumption.
Bleomycin sulfate (Blenoxane) is an anticancer agent for squamous cell carcinomas with IC50 of 4 nM in UT-SCC-19A cells. Bleomycin sulfate inhibits DNA synthesis and causes DNA scissions at specific base sequences. Bleomycin sulfate also inhibits tumor angiogenesis. Bleomycin sulfate causes single and double-strand DNA breaks in tumor cells, which interrupts the cell cycle. Bleomycin is thought to achieve this by chelating metal ions, producing a pseudoenzyme that reacts with oxygen to produce superoxide and hydroxide free radicals that cleave DNA. Bleomycin sulfate-induced telomere instability in mammalian cells persists for several generations after exposure. Moreover, the appearance of telomere fusions in Bleomycin sulfate-exposed cells 10 days after treatment suggests that Bleomycin sulfate can induce delayed telomere instability.
J Invest Dermatol. 2024 Apr 3;S0022-202X(24)00274-4.
Neutrophil-driven M2-like macrophages are critical for skin fibrosis in a systemic sclerosis model
Bleomycin sulfate purchased from AbMole
FEBS Open Bio. 2024 Oct 27.
Interaction of RECQL4 with poly(ADP-ribose) is critical for the DNA double-strand break response in human cells
Bleomycin sulfate purchased from AbMole
Adv Biol (Weinh). 2023 Feb 12;e2200265.
COVID‐19 Metabolomic‐Guided Amino Acid Therapy Protects from Inflammation and Disease Sequelae
Bleomycin sulfate purchased from AbMole
research square. 2022 July 11.
Neutrophil-driven CD206hiMHCIIlo macrophages are critical for skin fibrosis during systemic sclerosis
Bleomycin sulfate purchased from AbMole
Cell Death Discov. 2022 Nov 26;8(1):466.
Mesenchymal stem cells alleviate systemic sclerosis by inhibiting the recruitment of pathogenic macrophages
Bleomycin sulfate purchased from AbMole
| Cell Experiment | |
|---|---|
| Cell lines | MIA PaCa-2 cell |
| Preparation method | Proliferation assay. The effect of EGCG and BLM on cell proliferation was determined by using TACS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay (Trevigen, Githersburg). The cells (2000 per well) were incubated with EGCG alone, BLM alone or combination of EGCG and BLM, in triplicate in a 96-well plate and then incubated for 2, 4 and 6 days at 37 °C. A MTT solution was added to each well and incubated for 2 h at 37 °C. An extraction buffer (20 % SDS and 50 % dimethylformamide) was added, and the cells were incubated overnight at 37 °C. The absorbance of the cell suspension was measured at 570 nm using a microplate reader (DAS Technologies, Chantilly, VA). This experiment was repeated twice, and the statistical analysis was performed to obtain the final values. |
| Concentrations | 0, 10 and 20 μM |
| Incubation time | 2, 4 and 6 days |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 1512.62 |
| Formula | C55H85N17O25S4 |
| CAS Number | 9041-93-4 |
| Solubility (25°C) | Water ≥ 80 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Related Animal Modeling Products |
|---|
| Sodium Thioglycolate
Sodium thioglycolate acts as reducing agent and is suitable for anaerobic and microaerophilic bacterial growth. Sodium thioglycolate is a commonly used reagent for bacteriological research to maintain reducing conditions in media. Thioglycolate can also protect enzymes against inactivation by maintaining protein thiol groups in the reduced state. Thioglycolate medium is frequently used in inflammation research to elicit a neutrophil and macrophage response in vivo. |
| Vancomycin-d10 2TFA salt
Vancomycin-d10 2TFA salt |
| Acetic acid-d4
Acetic acid-d4 |
| Myosin H Chain Fragment, mouse acetate
Myosin H Chain Fragment, mouse acetate salt is a fragment of the α-Myosin heavy chain peptide. Myosin H Chain Fragment can be used to induce experimental autoimmune myocarditis (EAM) mouse model. |
| Myosin H Chain Fragment, mouse
Myosin H Chain Fragment, mouse is a fragment of the α-Myosin heavy chain peptide. Myosin H Chain Fragment can be used to induce experimental autoimmune myocarditis (EAM) mouse model. |
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