Free shipping on all orders over $ 500

Bleomycin sulfate

Cat. No. M2100
Bleomycin sulfate Structure
Synonym:

Blenoxane; NSC125066; BLEO

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
5mg USD 80  USD80 In stock
10mg USD 130  USD130 In stock
25mg USD 290  USD290 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

Bleomycin sulfate (Blenoxane) is an anticancer agent for squamous cell carcinomas with IC50 of 4 nM in UT-SCC-19A cells. Bleomycin sulfate inhibits DNA synthesis and causes DNA scissions at specific base sequences. Bleomycin sulfate also inhibits tumor angiogenesis. Bleomycin sulfate causes single and double-strand DNA breaks in tumor cells, which interrupts the cell cycle. Bleomycin is thought to achieve this by chelating metal ions, producing a pseudoenzyme that reacts with oxygen to produce superoxide and hydroxide free radicals that cleave DNA. Bleomycin sulfate-induced telomere instability in mammalian cells persists for several generations after exposure. Moreover, the appearance of telomere fusions in Bleomycin sulfate-exposed cells 10 days after treatment suggests that Bleomycin sulfate can induce delayed telomere instability.

Product Citations
Protocol (for reference only)
Cell Experiment
Cell lines MIA PaCa-2 cell
Preparation method Proliferation assay.
The effect of EGCG and BLM on cell proliferation was determined by using TACS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay (Trevigen, Githersburg). The cells (2000 per well) were incubated with EGCG alone, BLM alone or combination of EGCG and BLM, in triplicate in a 96-well plate and then incubated for 2, 4 and 6 days at 37 °C. A MTT solution was added to each well and incubated for 2 h at 37 °C. An extraction buffer (20 % SDS and 50 % dimethylformamide) was added, and the cells were incubated overnight at 37 °C. The absorbance of the cell suspension was measured at 570 nm using a microplate reader (DAS Technologies, Chantilly, VA). This experiment was repeated twice, and the statistical analysis was performed to obtain the final values.
Concentrations 0, 10 and 20 μM
Incubation time 2, 4 and 6 days
Animal Experiment
Animal models
Formulation
Dosages
Administration
Chemical Information
Molecular Weight 1512.62
Formula C55H85N17O25S4
CAS Number 9041-93-4
Solubility (25°C) Water ≥ 80 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Bimonte S, et al. Infect Agent Cancer. Inhibitory effect of (-)-epigallocatechin-3-gallate and bleomycin on human pancreatic cancer MiaPaca-2 cell growth.

[2] Huang LS, et al. FASEB J. Targeting sphingosine kinase 1 attenuates bleomycin-induced pulmonary fibrosis.

[3] Banerjee ER, et al. Stem Cell Res Ther. Characterization of lung stem cell niches in a mouse model of bleomycin-induced fibrosis.

[4] Danesi CC, et al. Mutat Res. Genotoxicity testing of combined treatment with cisplatin, bleomycin, and 5-fluorouracil in somatic cells of Drosophila melanogaster.

[5] Paviolo NS, et al. Mutat Res. Telomere instability is present in the progeny of mammalian cells exposed to bleomycin.

Related Animal Modeling Products
Myosin H Chain Fragment, mouse acetate

Myosin H Chain Fragment, mouse acetate salt is a fragment of the α-Myosin heavy chain peptide. Myosin H Chain Fragment can be used to induce experimental autoimmune myocarditis (EAM) mouse model.

Myosin H Chain Fragment, mouse

Myosin H Chain Fragment, mouse is a fragment of the α-Myosin heavy chain peptide. Myosin H Chain Fragment can be used to induce experimental autoimmune myocarditis (EAM) mouse model.

Keyhole limpet hemocyanin (from prawns, 98%)

Keyhole limpet hemocyanin is a copper protein that binds reversibly to oxygen, and it is lime green when oxidized and white when reduced. Keyhole limpet hemocyanin has oxygen-carrying function, antiviral activity, antibacterial activity, and coagulation function.

Astragalus Polysacharin (70%)

Astragalus Polysacharin is a natural product from Astragalus membranaeus, used as adjuvant, attenuates TNF-α-induced insulin resistance by suppressing miR-721 and activating PPAR-γ and PI3K/Akt in 3T3-L1 adipocytes.

Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat

Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) is a minor component of CNS myelin.

  Catalog
Abmole Inhibitor Catalog




Keywords: Bleomycin sulfate, Blenoxane; NSC125066; BLEO supplier, Animal Modeling, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.