Tunicamycin

Biological Activity

Tunicamycin inhibits GlcNAc phosphotransferase (GPT).Tunicamycin blocks the formation of N-glycosidic linkages by inhibiting the first step in glycoprotein synthesis.Tunicamycin can be used to induce autophagy.Tunicamycin induces apoptosis in association with caspase-3 activation, generation of reactive oxygen species (ROS), and downregulation of survivin expression.P38 MAPK (mitogen-activated protein kinase) and the generation of ROS signaling pathway play crucial roles in TN-induced apoptosis in U937 cells.TN modulates multiple components of the apoptotic response of human leukemia cells and raise the possibility of a novel therapeutic strategy for hematological malignancies.

Product Citations

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  J Ethnopharmacol. 2022 October 5;296:115519.

  Lycium barbarum L. and Salvia miltiorrhiza Bunge protect retinal pigment epithelial cells through endoplasmic reticulum stress
  Tunicamycin purchased from AbMole

• 

  Research Square. 2022 Apr 13.

  Mesenchymal stem cells alleviate epithelial-to-mesenchymal transition by modulating the IRE1α branch of the endoplasmic reticulum stress response
  Tunicamycin purchased from AbMole

Chemical Information

Molecular Weight	816.89
Formula	C37H60N4O16
CAS Number	11089-65-9
Solubility (25°C)	DMSO 30 mg/mL
Storage	-20°C, protect from light, dry, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Lim EJ, et al. Apoptosis. Tunicamycin promotes apoptosis in leukemia cells through ROS generation and downregulation of survivin expression.

[2] Lauer ME, et al. J Biol Chem. Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.

[3] Duriez M, et al. J Biol Chem. The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated degradation pathway.