Free shipping on all orders over $ 500

Sorafenib

Cat. No. M3026
Sorafenib Structure
Synonym:

BAY 43-9006

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mM*1mL in DMSO USD 36  USD40 In stock
50mg USD 36  USD40 In stock
100mg USD 45  USD50 In stock
500mg USD 63  USD70 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
Biological Activity

Sorafenib is a multikinase inhibitor of Raf-1, B-Raf and VEGFR-2 with IC50 of 6 nM, 22 nM and 90 nM, respectively. Sorafenib induces autophagy and apoptosis. Sorafenib has anti-tumor activity. Sorafenib is also a ferroptosis activator.

Product Citations
Customer Product Validations & Biological Datas
Source Phytomedicine (2015). Figure 1. Sorafenib was purchased from AbMole (Shanghai, China)
Method Cell viability was determined by MTT assay
Cell Lines HCC HepG2
Concentrations 0~100 µM
Incubation Time 24, 48, 72 hours
Results PPI dose- and time-dependently inhibited the proliferation of HepG2 cells, with IC50 values of 1.047 ± 0.376µM within 24 h. Sorafenib was used as a positive control in HepG2 cells.
Protocol
Cell Experiment
Cell lines PLC/PRF/5, Hep3B, HepG2, HuH7
Preparation method PLC/PRF/5, Hep3B, HepG2 and HuH7 Cells were seeded in 96-well plates (3,000 cells/well) and incubated overnight for attachment, and were then treated with indicated agents in 10% FBS supplemented medium for 72 hours. The medium was replaced with MTT (0.5 mg/ml) at 37°C for 2 hours. After removal of medium, the cells were lysed with 200 μl per well dimethyl sulfoxide (DMSO), and absorbance at 570 nm was measured and the values of 50% inhibition concentration (IC50) for each drug were determined.
Concentrations 0, 0.25, 0.5,1, 2 µM
Incubation time 72 hours
Animal Experiment
Animal models PLC/PRF/5 xenograft model in female athymic nude mice of 4–5 weeks of age
Formulation Dissolved in Cremophor EL/ethanol (50:50; Sigma Cremophor EL, 95% ethanol) at 4 × concentration.
Dosages 60 mg/kg
Administration Po, qd
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 464.82
Formula C21H16ClF3N4O3
CAS Number 284461-73-0
Purity 100.0%
Solubility DMSO 45 mg/mL
Storage at -20°C
References

GSTZ1 sensitizes hepatocellular carcinoma cells to sorafenib-induced ferroptosis via inhibition of NRF2/GPX4 axis
Qiujie Wang, et al. Cell Death Dis. 2021 Apr 30;12(5):426. PMID: 33931597.

Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activation
Jialei Sun, et al. Redox Biol. 2021 May;41:101942. PMID: 33770521.

Broadening horizons: the role of ferroptosis in cancer
Xin Chen, et al. Nat Rev Clin Oncol. 2021 May;18(5):280-296. PMID: 33514910.

Sorafenib and DE605, a novel c-Met inhibitor, synergistically suppress hepatocellular carcinoma
Xiufeng Jiang, et,al. Oncotarget, 2015,6;14.

Related Raf Products
Lifirafenib (BGB-283)

Lifirafenib (BGB-283) is a novel potent and selective RAF Kinase and EGFR inhibitor with IC50 values of 23, 29 and 495 nM for the recombinant BRAFV600E kinase domain, EGFR and EGFR T790M/L858R mutant.

BI 882370

BI 882370 is a highly potent and selective RAF inhibitor that binds to the DFG-out (inactive) conformation of the BRAF kinase.

Naporafenib

Naporafenib (also known as LXH254) is an orally available inhibitor of C-Raf and B-Raf with potential antineoplastic activity.

B-Raf inhibitor 1

B-Raf inhibitor 1 is a potent and selective B-Raf inhibitor with cell IC50s of 0.31 µM and 2 nM for A375 proliferation and A375 p-ERK, respectively.

PLX7904

PLX7904, also known as PB04, is a potent and selective paradox-breaker RAF inhibitor.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Sorafenib, BAY 43-9006 supplier, Raf, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.