All AbMole products are for research use only, cannot be used for human consumption.
Temsirolimus (CCI-779) inhibited the growth of xenografts derived from both cell lines with greater effects against PC-3 than DU145 tumors. Temsirolimus caused mild myelosuppression. The activity of mitoxantrone or docetaxel was limited, but CCI-779 given between courses of chemotherapy increased growth delay of PC-3 xenografts. High-dose temsirolimus inhibits mTOR signaling through an FKBP12-independent mechanism that leads to profound translational repression. This distinctive high-dose compound effect could be directly related to the antitumor activities of temsirolimus and other rapalogues in human cancer patients. The rapamycin analogue CCI-779 is an important new agent to investigate in the treatment of human brain tumors, particularly PNET/MB. The rapamycin analogue temsirolimus improved performance on four different behavioral tasks and decreased aggregate formation in a mouse model of Huntington disease.
Int J Nanomedicine. 2024 Sep 23;19:9821-9841.
pH-Responsive Block Copolymer Micelles of Temsirolimus: Preparation, Characterization and Antitumor Activity Evaluation
Temsirolimus purchased from AbMole
Journal of Pharmaceutical and Biomedical Analysis. 2023 Aug 14;115652.
Determination of five mTOR inhibitors in human plasma for hepatocellular carcinoma treatment using QuEChERS-UHPLC-MS/MS
Temsirolimus purchased from AbMole
Mol Cell. 2018 Dec 3.
Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression.
Temsirolimus purchased from AbMole
Cell Death and Disease. 2018 Jan 26;9:137-52.
Downregulation of MCL-1 and upregulation of PUMA using mTOR inhibitors enhance antitumor efficacy of BH3 mimetics in triple-negative breast cancer
Temsirolimus purchased from AbMole
J Agric Food Chem. 2017 Mar 15;65(10):2089-2099.
10-Gingerol, a Phytochemical Derivative from “Tongling White Ginger”, Inhibits Cervical Cancer: Insights into the Molecular Mechanism and Inhibitory Targets
Temsirolimus purchased from AbMole
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Source | Cell Death & Disease (2018). Figure 2. Temsirolimus (Abmole Bioscience) |
| Method | colony formation assays | |
| Cell Lines | MDA-MB-231, MDA-MB-157, and MDA-MB-468 cells | |
| Concentrations | 1 μM | |
| Incubation Time | 24 h | |
| Results | But treatment with Temsirolimus in combination with ABT263 or ABT199 only induced modest cell viability inhibition. |
 |
Source | Cell Death & Disease (2018). Figure 1. Temsirolimus (Abmole Bioscience) |
| Method | Immunoblotting analysis | |
| Cell Lines | TNBC cell lines | |
| Concentrations | 1 μM | |
| Incubation Time | 24 h | |
| Results | As expected, we found the mTOR inhibitors, especially the BEZ235 and AZD8055, could significantly inhibit the phosphorylation of AKT and 4EBP1 and efficiently led to decreasing MCL-1 expression. |
. figure 7.jpg) |
Source | APMIS.m (2017). Figure 7. temsirolimus (Abmole Bioscience Inc. Houston, TX, USA) |
| Method | Western blot | |
| Cell Lines | HeLa cells | |
| Concentrations | ||
| Incubation Time | ||
| Results | In addition, exposure with deguelin and temsirolimus could weaken the inhibitory attributes of 10-G on the expression of p-AKT and p-mTOR. |
| Cell Experiment | |
|---|---|
| Cell lines | A549, H157, H460, H446, HCT116, HT29, SW480, DLD1, Caco2, LNCap, DU145, MDA468, MDA231, HEK293, and PC3-MM2 |
| Preparation method | Exposing cells to various concentrations of Temsirolimus for 72 hours. After treatment, using CellTiter AQ assay kit to determing viable cell densities by MTS dye conversion. |
| Concentrations | Dissolved in DMSO, final concentrations ~20 μM |
| Incubation time | 72 hours |
| Animal Experiment | |
|---|---|
| Animal models | Female athymic nude mice injected s.c. with DAOY, or U251 cells |
| Formulation | Prepared in 100% EtOH as a 50 mg/mL stock solution, and diluted in 5% Tween 80 and 5% polyethylene glycol 400 |
| Dosages | 20 mg/kg |
| Administration | Injection daily 5 times per week |
| Molecular Weight | 1030.29 |
| Formula | C56H87NO16 |
| CAS Number | 162635-04-3 |
| Solubility (25°C) | DMSO ≥40 mg/mL Ethanol ≥50 mg/mL |
| Storage | -20°C, protect from light, sealed |
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| WYE-687 dihydrochloride
WYE-687 dihydrochloride is an ATP-competitive mTOR inhibitor with an IC50 of 7 nM. |
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Products are for research use only. Not for human use. We do not sell to patients.
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