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Pyrrolidinedithiocarbamate ammonium (PDTC)

Cat. No. M4005
Pyrrolidinedithiocarbamate ammonium (PDTC) Structure
Synonym:

Ammonium pyrrolidinedithiocarbamate; APDC

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
50mg USD 27  USD30 In stock
100mg USD 45  USD50 In stock
200mg USD 72  USD80 In stock
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Quality Control
Biological Activity

Pyrrolidinedithiocarbamate ammonium (PDTC) can enhance the cell apoptosis induced by TNF-α, which may be associated with the blocking of TNF-α-activated NF-κB signaling pathway by PDTC, the down-regulation of survivin expression, and up-regulation of caspase-3 expression.in NMSC PDTC acts as pro-oxidant and enhances IR-induced cell cytotoxicity by increasing H(2)O(2)formation and thiol oxidation.PDTC and MG132, inhibitors of NF-kappaB, block ET-induced vasodilation in isolated rat skeletal muscle arterioles.PDTC has effects at both the level of the aortic segment as well as the resistance arteriole.PDTC prevents induction of nitric oxide synthase (NOS) by inhibiting translation of NOS mRNA.PDTC induces apoptosis in rat smooth muscle cells and inhibits apoptosis in leukemia HL-60 cells.

Product Citations
Customer Product Validations & Biological Datas
Source Arthritis Research & Therapy (2018). Figure 5. PDTC (Abmole Bioscience)
Method qRT-PCR
Cell Lines Synovial tissues
Concentrations
Incubation Time 24h
Results qRT-PCR showed that expression of IL-1α (Fig. 5r, u), IL-1β (Fig. 5s, v), and TNF-α (Fig. 5t, w) in response to LPS was significantly attenuated by PDTC and LY294002.
Source Veterinary Microbiology (2018). Figure 6. PDTC (Abmole Bioscience, Houston, TX, USA)
Method Detection of DC activation
Cell Lines DCs
Concentrations 20 μM
Incubation Time 2 h
Results The results indicated that Lp-HA-DCpep is more efficient in being englobed by DCs than Lp-HA and Lp determined by the FITC-Dextran uptake experiment (Fig. 6A) and thereby more effective in activating T cells as showed by MLR.
Source J Infect Dis (2018). Figure 7. PDTC (Abmole Bioscience)
Method Western blotting
Cell Lines mast cells
Concentrations 25 μM
Incubation Time 30 minutes
Results The S. aureus-induced production of TNF-α and CRAMP was significantly reduced by pretreatment with the specific PI3K inhibitor Wortmannin or the specific NF-κB inhibitor PDTC
Source Sci Rep (2017). Figure 4. PDTC (AbMole BioScience Inc., Brussels, Belgium)
Method Inhibitors of innate signalling pathways
Cell Lines HEK cells
Concentrations 100 μM
Incubation Time 24 h
Results As expected, RELA knock-down and the presence of PDTC, an inhibitor of NFκB, resulted in substantial reduction of CXCL8 production in response to H7 flagellin by hTLR5 in the HEK background (p < 0.05)
Source Vete Mic (2017). Figure 4. PDTC (Abmole Bioscience, Houston, TX, USA)
Method Treatment by inhibitors
Cell Lines DCs
Concentrations 20 µM
Incubation Time 48 h
Results The PDTC (NF-kB inhibitor) treatment significantly decreased the MHC II, CD80, CD86, CD40 expressions. As to cytokine production (Fig. 4e–h), both inhibitors significantly restrained TNF-a, IFN-g and IL-6 secretions, with a minimal effect on IL-12p70.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 164.29
Formula C5H9NS2·NH3
CAS Number 5108-96-3
Purity 99.04%
Solubility Water 30 mg/mL
DMSO 30 mg/mL
Storage at -20°C
References

Epoxymicheliolide, a novelguaiane-type sesquiterpene lactone, inhibits NF‑κB/COX‑2 signaling pathways by targeting leucine 281 and leucine 25 in IKKβ in renal cell carcinoma
Jiabin Zhu, et al. Int J Oncol. 2018 Sep;53(3):987-1000. PMID: 29956738.

[NF-κb inhibitor PDTC enhances tumor necrosis factor α-induced apoptosis of gastric cancer cell SGC-7901].
Cao MZ, et al. Zhonghua Wei Chang Wai Ke Za Zhi. 2013 Jun;16(6):578-82. PMID: 23801215.

Pyrrolidine dithiocarbamate (PDTC) blocks apoptosis and promotes ionizing radiation-induced necrosis of freshly-isolated normal mouse spleen cells.
Thompson JS, et al. Apoptosis. 2010 Jun;15(6):705-14. PMID: 20333468.

PDTC and Mg132, inhibitors of NF-kappaB, block endotoxin induced vasodilation of isolated rat skeletal muscle arterioles.
Snyder JG, et al. Shock. 2002 Apr;17(4):304-7. PMID: 11954831.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Pyrrolidinedithiocarbamate ammonium (PDTC), Ammonium pyrrolidinedithiocarbamate; APDC supplier, NF-κB, inhibitors

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