Puromycin dihydrochloride

Biological Activity

Puromycin dihydrochloride is a protein synthesis inhibitor. Puromycin 2HCl leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Puromycin dihydrochloride Inhibits translation in both in vitro and in vivo systems. Puromycin dihydrochloride inhibits the transport of proteins into the mitochondria in vitro. Puromycin inhibits protein import into mitochondria by interfering with an ATP-dependent step in the import process and that the ATP-dependent component in the reaction is located inside the inner mitochondrial membrane.

Product Citations

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  Transl Oncol. 2023 Feb;28;101617.

  KIAA1199 promotes oxaliplatin resistance and epithelial mesenchymal transition of colorectal cancer via protein O-GlcNAcylation
  Puromycin dihydrochloride purchased from AbMole

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  J Ethnopharmacol. 2023 Feb 10;302(Pt A):115897.

  Total glucosides of paeony alleviates scleroderma by inhibiting type I interferon responses
  Puromycin dihydrochloride purchased from AbMole

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  Int J Chron Obstruct Pulmon Dis. 2021 Jun 4;16:1661-1675.

  Histone Deacetylase 2 Suppresses Skeletal Muscle Atrophy and Senescence via NF-κB Signaling Pathway in Cigarette Smoke-Induced Mice with Emphysema
  Puromycin dihydrochloride purchased from AbMole

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  Nucleic Acids Res. 2021 Mar 18;49(5):2848-2858.

  HuB and HuD repress telomerase activity by dissociating HuR from TERC
  Puromycin dihydrochloride purchased from AbMole

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  DNA Cell Biol. 2021 Jul;40(7):895-905.

  AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through Impairing Homologous Recombination Via Targeting RFC5
  Puromycin dihydrochloride purchased from AbMole

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  Nature. 2020 Dec;588(7839):664-669.

  Creation of bladder assembloids mimicking tissue regeneration and cancer
  Puromycin dihydrochloride purchased from AbMole

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  Cell Death Dis. 2020 Oct 23;11(10):902.

  The miR-30a-5p/CLCF1 axis regulates sorafenib resistance and aerobic glycolysis in hepatocellular carcinoma
  Puromycin dihydrochloride purchased from AbMole

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  Environ Health Perspect. 2020 Jun;128(6):67008.

  Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation
  Puromycin dihydrochloride purchased from AbMole

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  Exp Biol Med (Maywood). 2020 Jun;245(11):925-932.

  Circular RNA circ-CDYL sponges miR-1180 to elevate yes-associated protein in multiple myeloma
  Puromycin dihydrochloride purchased from AbMole

Chemical Information

Molecular Weight	544.43
Formula	C22H29N7O5.2HCl
CAS Number	58-58-2
Solubility (25°C)	Water ≥ 30 mg/mL
Storage	-20°C, dry, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Price J, et al. Biochim Biophys Acta. Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction.