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Gemcitabine

Cat. No. M5680
Gemcitabine Structure
Synonym:

NSC 613327; LY188011

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mM*1mL in DMSO USD 22.5  USD25 In stock
50mg USD 27  USD30 In stock
100mg USD 40.5  USD45 In stock
200mg USD 63  USD70 In stock
500mg USD 85.5  USD95 In stock
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Quality Control
Biological Activity

Gemcitabine(LY-188011; NSC 613327) is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Product Citations
Customer Product Validations & Biological Datas
Source Biosci Rep (2020), Figure 2, Gemcitabine (Abmole Bioscience)
Method MTT assay for cell cytotoxicity
Cell Lines TNBC cell lines
Concentrations 0, 1, 3, 5 nm
Incubation Time 48 h
Results Next, the cytotoxicity in each group was monitored with MTT when the cells were treated with 0, 1, 3 and 5 nm gemcitabine and findings tended to prove that agonized NLRP3 could better resist the inhibitory effect of growing gemcitabine, in other words, higher expression of NLRP3 decreased the cytotoxicity and added to the gemcitabine resistance while inhibition of NLRP3 increased cytotoxicity in TNBCC, helping to alleviate the drug resistance
Source Biosci Rep (2020), Figure 1, Gemcitabine (Abmole Bioscience)
Method Cell culture and treatment
Cell Lines TNBC cell lines
Concentrations 0, 1, 3, 5 nM
Incubation Time 72 h
Results The linear graph presented the cytotoxicity at 72 h when exposed to different concentrations. It showed that highest cell viability was seen in agonist group with higher NLRP3 and lowest in antagonist group, suggesting that the higher expression of NLRP3, the less sensitive to different gemcitabine doses
Source Bioscience Reports (2020 Jul). Figure 1. Gemcitabine (Abmole Bioscience)
Method Cell culture
Cell Lines TNBC cell lines
Concentrations 0, 1, 3, 5 nM
Incubation Time 72 h
Results It showed that highest cell viability was seen in agonist group with higher NLRP3 and lowest in antagonist group, suggesting that the higher expression of NLRP3, the less sensitive to different gemcitabine doses
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 263.2
Formula C9H11F2N3O4
CAS Number 95058-81-4
Purity 99.95%
Solubility DMSO ≥ 30 mg/mL
Storage -20°C, protect from light, sealed
References

Gemcitabine resistance in pancreatic ductal adenocarcinoma.
Binenbaum Y, et al. Drug Resist Updat. 2015 Nov;23:55-68. PMID: 26690340.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Gemcitabine, NSC 613327; LY188011 supplier, DNA/RNA Synthesis, inhibitors

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