DAPI dihydrochloride

Biological Activity

DAPI dihydrochloride is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.

Product Citations

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  Appl Biochem Biotechnol Appl Biochem Biotechnol. 2022 Jul 1.

  Apatinib Through Activating the RhoA/ROCK Signaling Pathway to Cause Dysfunction of Vascular Smooth Muscle CellsMuscle Cells
  DAPI dihydrochloride purchased from AbMole

• 

  Adv Mater. 2023 Feb 22;e2211138.

  Genetically Programmable Vesicles for Enhancing CAR-T Therapy Against Solid Tumors
  DAPI dihydrochloride purchased from AbMole

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  Nucleic Acids Res . 2022 Aug 12;50(14):7938-7958.

  HDAC1 and PRC2 mediate combinatorial control in SPI1/PU.1-dependent gene repression in murine erythroleukaemia
  DAPI dihydrochloride purchased from AbMole

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  Oncol Lett. 2022 May;23(5):163.

  AK4P1 is a cancer-promoting pseudogene in pancreatic adenocarcinoma cells whose transcripts can be transmitted by exosomes
  DAPI dihydrochloride purchased from AbMole

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  Ann Biomed Eng. 2021 Jun;49(6):1551-1560.

  Comparison of in Vascular Bioreactors and In Vivo Models of Degradation and Cellular Response of Mg-Zn-Mn Stents
  DAPI dihydrochloride purchased from AbMole

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  Reprod Sci. 2021 Jun 2.

  LncRNA HOXA-AS2 Activates the Notch Pathway to Promote Cervical Cancer Cell Proliferation and Migration
  DAPI dihydrochloride purchased from AbMole

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  Theranostics. 2020 Apr 27;10(13):5671-5686.

  SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma
  DAPI dihydrochloride purchased from AbMole

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  Oncology. 2020 Jul 20.

  5-Fluorouracil enhances the chemosensitivity of gastric cancer to TRAIL via inhibition of the MAPK pathway
  DAPI dihydrochloride purchased from AbMole

• 

  Life Sci. 2020 Sep 15;257:118021.

  TRIB3 destabilizes tumor suppressor PPARα expression through ubiquitin-mediated proteasome degradation in acute myeloid leukemia
  DAPI dihydrochloride purchased from AbMole

• 

  Anat Rec (Hoboken). 2020 Jan 21.

  Resibufogenin inhibited colorectal cancer cell growth and tumorigenesis through triggering ferroptosis and ROS production mediated by GPX4 inactivation
  DAPI dihydrochloride purchased from AbMole

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  Aging (Albany NY). 2020 Jun 9;12(11):11025-11041.

  LncRNA ADAMTS9-AS2 inhibits gastric cancer (GC) development and sensitizes chemoresistant GC cells to cisplatin by regulating miR-223-3p/NLRP3 axis
  DAPI dihydrochloride purchased from AbMole

Chemical Information

Molecular Weight	350.25
Formula	C16H17Cl2N5
CAS Number	28718-90-3
Solubility (25°C)	Water 5 mg/mL (Need ultrasonic)
Storage	-20°C, protect from light, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Qunxiong Wu, et al. Reprod Sci. LncRNA HOXA-AS2 Activates the Notch Pathway to Promote Cervical Cancer Cell Proliferation and Migration