BAPTA-AM

Biological Activity

BAPTA-AM exerts an open channel blocking effect on hERG, hKv1.3 and hKv1.5 channels with IC50 of 1.3, 1.45 and 1.23 μM in HEK 293 cells. BAPTA-AM, as an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. BAPTA-AM prevents free radical-mediated toxicity promote apoptosis in non-neuronal cells and produce a beneficial effect in neuronal cells by protecting neurons from ischemic damage. In addition, it has been suggested that BAPTA-AM induces a late, but not early, increase of intracellular calcium in I-IL-60 neoplastic cells. Mixed cortical cell cultures (DIV 13-16) exposed to 10 μM BAPTA-AM for 24- or 48-hr show moderate (45-70%) neuronal injury as evaluated by increased LDH release into the bathing medium after 24-48-hr.

Product Citations

• 

  Neurochem Res. 2023 Feb;48(2):447-457.

  Ca2+ Regulates Autophagy Through CaMKKβ/AMPK/mTOR Signaling Pathway in Mechanical Spinal cord Injury: An in vitro Study
  BAPTA-AM purchased from AbMole

• 

  Ecotoxicol Environ Saf. 2021 Sep 1;220:112341.

  Cadmium induces apoptosis of human granulosa cell line KGN via mitochondrial dysfunction-mediated pathways
  BAPTA-AM purchased from AbMole

• 

  Ecotoxicol Environ Saf. 2021 Jan 15;208:111429.

  Bisphenol A induces apoptosis through GPER-dependent activation of the ROS/Ca 2+-ASK1-JNK pathway in human granulosa cell line KGN
  BAPTA-AM purchased from AbMole

• 

  Environ Pollut. 2021 Feb 1;270:116051.

  Bisphenol AF induces apoptosis via estrogen receptor beta (ERβ) and ROS-ASK1-JNK MAPK pathway in human granulosa cell line KGN
  BAPTA-AM purchased from AbMole

Customer Product Validations & Biological Datas

	Source	ECOTOX ENVIRON SAFE 2021 Jan. Figure 5. BAPTA-AM (Abmole Bioscience Inc, Houston, TX, USA)
	Method	cell culture
	Cell Lines	KGN cells
	Concentrations	10 μM
	Incubation Time	1h
	Results	We further pretreated KGN cells with BAPTA-AM (a calcium chelator) and found that BPA-induced phosphorylation of JNK and ASK1 proteins and apoptosis of KGN cells was significantly inhibited

Chemical Information

Molecular Weight	764.68
Formula	C34H40N2O18
CAS Number	126150-97-8
Solubility (25°C)	DMSO 40 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Tang Q,et.al. Biochem Pharmacol The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells.