Free shipping on all orders over $ 500
SN-38 is an active metabolite of CPT-11, inhibits DNA topoisomerase I, DNA synthesis and causes frequent DNA single-strand breaks.
BMC Biol. 2021 May 20;19(1):108.
Very long intergenic non-coding (vlinc) RNAs directly regulate multiple genes in cis and trans
SN-38 purchased from AbMole
Nat Commun. 2019 Dec 20;10(1):5799.
Novel approach reveals genomic landscapes of single-strand DNA breaks with nucleotide resolution in human cells.
SN-38 purchased from AbMole
 |
Source | Customer experimental result (2018). |
| Method | Cell viability assay | |
| Cell Lines | MDA-MB-231 cells | |
| Concentrations | 32nM | |
| Incubation Time | 4 days | |
| Results | MDA-MB-231 cells were treated with 32nM SN-38 for 4 days and then incubated for 4 days. Most of the MDA-MB-231 cells were transformed into senescent cells. |
 |
Source | J Immunol Res (2018). Figure 1. SN-38 |
| Method | flow cytometry | |
| Cell Lines | NB cell lines | |
| Concentrations | 1 nM | |
| Incubation Time | 24 hours | |
| Results | Cisplatin at 2 μM, etoposide at 0.1 μM, irinotecan at 1 nM, and topotecan at 10nM induced apoptosis of all NB cell lines after 48 hours of treatment, with the exception of SK-N-AS treated with the first three drugs and SK-N-BE(2)c treated with all four drugs |
| Cell Experiment | |
|---|---|
| Cell lines | A-172, U-87, and LA-567 |
| Preparation method | MTT assay |
| Concentrations | 0 -1000 nM |
| Incubation time | 48 h |
| Animal Experiment | |
|---|---|
| Animal models | Nontumor-bearing mice |
| Formulation | dissolved in DMSO (1 mg/mL) |
| Dosages | 1 mg/kg |
| Administration | i.v. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Molecular Weight | 392.4 |
| Formula | C22H20N2O5 |
| CAS Number | 86639-52-3 |
| Purity | 99.57% |
| Solubility | DMSO 21 mg/mL |
| Storage | at -20°C |
| Related Topoisomerase Products |
|---|
| Pyrazoloacridine
Pyrazoloacridine (NSC 366140) has anticancer activity, inhibiting the activity of topoisomerases 1 and 2 (topoisomerases 1 and 2). Pyrazoloacridine (NSC 366140) on K562 in myeloid leukemia cells IC50 The value is 1.25 μM (24 h). |
| Berberine chloride hydrate
Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) is an alkaloid commonly used as an antibiotic. Berberine chloride hydrate induces reactive oxygen species (ROS) production and inhibits DNA topoisomerase. Anti-tumor properties. |
| PluriSIn #2
PluriSIn #2 is a selective transcriptional inhibitor of topoisomerase II α (TOP2A). PluriSIn #2 is a compound that selectively eliminates undifferentiated human pluripotent stem cells (hPSCs). |
| LMP744 hydrochloride
LMP744 hydrochloride (MJ-III65 hydrochloride) is a DNA chimeric agent that is an inhibitor of topoisomerase 1 (Top1) with antitumor activity. |
| Alternariol
Alternariol is a mycotoxin produced by Alternaria species. Alternariol inhibits the catalytic activity of topoisomerase I and topoisomerase II enzymes. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.
