SN-38 is an active metabolite of CPT-11, inhibits DNA topoisomerase I, DNA synthesis and causes frequent DNA single-strand breaks.
BMC Biol. 2021 May 20;19(1):108.
Very long intergenic non-coding (vlinc) RNAs directly regulate multiple genes in cis and trans
SN-38 purchased from AbMole
Nat Commun. 2019 Dec 20;10(1):5799.
Novel approach reveals genomic landscapes of single-strand DNA breaks with nucleotide resolution in human cells.
SN-38 purchased from AbMole
|Source||Customer experimental result (2018).|
|Method||Cell viability assay|
|Cell Lines||MDA-MB-231 cells|
|Incubation Time||4 days|
|Results||MDA-MB-231 cells were treated with 32nM SN-38 for 4 days and then incubated for 4 days. Most of the MDA-MB-231 cells were transformed into senescent cells.|
|Source||J Immunol Res (2018). Figure 1. SN-38|
|Cell Lines||NB cell lines|
|Incubation Time||24 hours|
|Results||Cisplatin at 2 μM, etoposide at 0.1 μM, irinotecan at 1 nM, and topotecan at 10nM induced apoptosis of all NB cell lines after 48 hours of treatment, with the exception of SK-N-AS treated with the first three drugs and SK-N-BE(2)c treated with all four drugs|
|Cell lines||A-172, U-87, and LA-567|
|Preparation method||MTT assay|
|Concentrations||0 -1000 nM|
|Incubation time||48 h|
|Animal models||Nontumor-bearing mice|
|Formulation||dissolved in DMSO (1 mg/mL)|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
|Solubility||DMSO 21 mg/mL|
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