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Camptothecin (CPT), an alkaloid, is an inhibitor of DNA Topoisomerase I (Topo I) with an IC50 value of 679 nM. Camptothecin (CPT) has strong antitumor activity against colorectal cancer, breast cancer, lung cancer and ovarian cancer, and modulates hypoxia-inducible factor-1α (HIF-1α) activity by altering miRNA expression patterns in human cancer cells.
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Camptothecin purchased from AbMole
| Cell Experiment | |
|---|---|
| Cell lines | HCT116 |
| Preparation method | Briefly, cells were incubated with BrdUrd (24 h for normal cells, and 72 h for CPT-treated cells), fixed for 20 min in 2% glutaraldehyde in PBS at room temperature, and the cell membranes were permeabilized with acetone and methanol (1:1). The cells were then incubated for 30 min in 2 m HCl followed by a 30-min incubation in 0.1 m sodium borate (pH 8.5), washed three times in PBS, incubated for 60 min in 1% bovine serum albumin/PBS, and followed by a 45-min incubation in PBS containing 2 μg/ml mouse monoclonal antibody. |
| Concentrations | 20 nM, 250 nM |
| Incubation time | 72 h |
| Animal Experiment | |
|---|---|
| Animal models | CDF1 female mice |
| Formulation | Polyethylene glycol 400, propylene glycol and polysorbate 80 (40:50:2, volume ratio) |
| Dosages | 2.5 mg/kg |
| Administration | Intravenously administered |
| Molecular Weight | 348.36 |
| Formula | C20H16N2O4 |
| CAS Number | 7689-03-4 |
| Solubility (25°C) | DMSO 3 mg/mL |
| Storage | 2-8°C, protect from light |
[1] Liu LF, et al. Ann N Y Acad Sci. Mechanism of action of camptothecin.
| Related Topoisomerase Products |
|---|
| Amonafide
Amonafide is a unique ATP-independent Topo II inhibitor that is being studied in the treatment of cancer. |
| Ellagic acid
Ellagic acid is a natural antioxidant as well as a potent, ATP-competitive inhibitor of CK2 and SHP2 with IC50 and Ki values of 40 nM and 20 nM, respectively.In addition, Ellagic acid exhibits antiproliferative activity and inhibition of topoisomerase II (Topoisomerase II). |
| Pirarubicin
Pirarubicin is an anthracycline that is an inhibitor of Topoisomerase II.*The compound is unstable in solutions, freshly prepared is recommended |
| β-Lapachone
β-Lapachone is a selective DNA topoisomerase I inhibitor, exhibiting no inhibitory activities against DNA topoisomerase II or ligase. |
| Flumequine
Flumequine is a synthetic chemotherapeutic antibiotic, inhibiting topoisomerase II with IC50 of 15 μM. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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