Blebbistatin is a cell-permeable inhibitor for non muscle myosin II ATPase with IC50 of ~2 μM, does not inhibit myosin light chain kinase, inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly.
*The compound is unstable in solutions, freshly prepared is recommended
Lab Chip. 2023 Jul 7.
A microfluidic platform integrating dynamic cell culture and dielectrophoretic manipulation for in situ assessment of endothelial cell mechanics
(-)-Blebbistatin purchased from AbMole
Molecular Weight | 292.33 |
Formula | C18H16N2O2 |
CAS Number | 856925-71-8 |
Solubility (25°C) | DMSO 10 mg/mL |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
Related Myosin Products |
---|
Myosin Light Chain Kinase Substrate (smooth muscle)
Myosin Light Chain Kinase Substrate (smooth muscle) is a smooth muscle myosin light chain kinase (MLCK) synthetic peptide substrate. |
Kemptamide
Kemptamide, a 13 amino acid fragment with a sequence corresponding to residues 11-23 of gizzard light chain. |
MYK-224
MYK-224 is a cardiac myosin complex modulator that can be used in studies related to cardiovascular disease. |
Myosin V-IN-1
Myosin V-IN-1 is a potent and selective Myosin V inhibitor, with a Ki of 6 μM. |
16-38-Thymosin β4 (cattle) TFA
16-38-Thymosin β4 (cattle) TFA is a non-Ca2+-dependent MLCK activator with high affinity. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.