(±)-Blebbistatin (BLEB) is a small cell permeable selective myosin II inhibitor. Rabbit arterial SM was potently inhibited by BLEB (IC50 ∼5 µM) although this tissue does not express significant amounts of NMM II. Blebbistatin (BLEB) inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Blebbistatin (BLEB) rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells.
Due to the known light sensitivity of Blebbistatin (BLEB), it was always kept in the dark in the refrigerator until just prior to usage.
Sci China Life Sci. 2022 Oct;65(10):2031-2049.
Mechanical transmission enables EMT cancer cells to drive epithelial cancer cell migration to guide tumor spheroid disaggregation
Blebbistain purchased from AbMole
| Molecular Weight | 292.34 |
| Formula | C18H16N2O2 |
| CAS Number | 674289-55-5 |
| Solubility (25°C) | DMSO ≥ 15 mg/mL |
| Storage | -20°C, protect from light, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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