All AbMole products are for research use only, cannot be used for human consumption.
In vitro: HepG2 cells are treated with various concentrations of AICAR (0.1-1.0 mM) for 12, 24, and 48 h, respectively. The expression level of IR-β significantly decreases with 0.25, 0.5, and 1.0 mM of AICAR at 48 h to 50%, 53%, and 46% of the control, respectively.
In vivo: Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type andob/ob (O; 59.6 g body wt) mice are injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg/g per day or saline control (C) for 14 days. At 24 h after the last injection (including a 12-h fast), all mice are killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) is excised for analysis. Muscle mass is lower in OC (159±12 mg) than LC, LA, and OA (176±10, 178±9, and 166±16 mg, respectively) mice, independent of a body weight change. The kidney weight is significantly higher in the untreated group when compared with both the exercise and AICAR (0.5 mg/g body wt) groups. The heart weight is higher in the exercise group than in the other groups, whereas the liver weight is significantly higher in the AICAR-treated group when compared with the exercise and untreated groups.
BMC Pulm Med. 2024 May 8;24(1):224.
Simvastatin attenuates silica-induced pulmonary inflammation and fibrosis in rats via the AMPK-NOX pathway
AICAR purchased from AbMole
Front Oncol. 2022 Jul 28;12:968547.
Comprehensive analysis of the glutathione S-transferase Mu (GSTM) gene family in ovarian cancer identifies prognostic and expression significance
AICAR purchased from AbMole
Research Square. 2022 May.
Perfluorooctane sulfonic acid induces liver lipid accumulation through AMPK/ACC signaling
AICAR purchased from AbMole
Cell Experiment | |
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Cell lines | K562 cell lines |
Preparation method | Acadesine is added to K562 cell lines or primary cells (103 CD34+ cells/mL) growing in semisolid methyl cellulose medium. MethoCult H4100 or H4236 are used for cell lines and primary CD34+ cells respectively. Colonies are detected after 10 days of culture by adding 1 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent and are scored by Image J quantification software. |
Concentrations | 2.5 mM |
Incubation time | 10 days |
Animal Experiment | |
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Animal models | mouse xenograft model of K562 cells |
Formulation | 0.9% NaCl |
Dosages | 50 mg/kg |
Administration | Injected intraperitoneally |
Molecular Weight | 258.23 |
Formula | C9H14N4O5 |
CAS Number | 2627-69-2 |
Solubility (25°C) | DMSO ≥ 50 mg/mL Water 20 mg/mL |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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