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Cat. No. M4897
AICAR Structure

Acadesine; AICA Riboside

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
50mg USD 40  USD40 In stock
100mg USD 70  USD70 In stock
200mg USD 120  USD120 In stock
500mg USD 250  USD250 In stock
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Quality Control & Documentation
Biological Activity

In vitro: HepG2 cells are treated with various concentrations of AICAR (0.1-1.0 mM) for 12, 24, and 48 h, respectively. The expression level of IR-β significantly decreases with 0.25, 0.5, and 1.0 mM of AICAR at 48 h to 50%, 53%, and 46% of the control, respectively.

In vivo: Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type andob/ob (O; 59.6 g body wt) mice are injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg/g per day or saline control (C) for 14 days. At 24 h after the last injection (including a 12-h fast), all mice are killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) is excised for analysis. Muscle mass is lower in OC (159±12 mg) than LC, LA, and OA (176±10, 178±9, and 166±16 mg, respectively) mice, independent of a body weight change. The kidney weight is significantly higher in the untreated group when compared with both the exercise and AICAR (0.5 mg/g body wt) groups. The heart weight is higher in the exercise group than in the other groups, whereas the liver weight is significantly higher in the AICAR-treated group when compared with the exercise and untreated groups.

Product Citations
Customer Product Validations & Biological Datas
Source Sci Rep (2018). Figure 1. AICAR
Method Cytometric bead array assays
Cell Lines Human peripheral blood mononuclear cells
Concentrations 1 mM
Incubation Time 24 h
Results Inhibition of adenosine kinase-mediated AICAR phosphorylation to ZMP, using the inhibitor ABT-702, left suppression of LPS-induced target genes by AICAR unaltered and even potentiated the effect of low AICAR concentrations, suggesting an AMPK-independent effect.
Protocol (for reference only)
Cell Experiment
Cell lines K562 cell lines
Preparation method Acadesine is added to K562 cell lines or primary cells (103 CD34+ cells/mL) growing in semisolid methyl cellulose medium. MethoCult H4100 or H4236 are used for cell lines and primary CD34+ cells respectively. Colonies are detected after 10 days of culture by adding 1 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent and are scored by Image J quantification software.
Concentrations 2.5 mM
Incubation time 10 days
Animal Experiment
Animal models mouse xenograft model of K562 cells
Formulation 0.9% NaCl
Dosages 50 mg/kg
Administration Injected intraperitoneally
Chemical Information
Molecular Weight 258.23
Formula C9H14N4O5
CAS Number 2627-69-2
Solubility (25°C) DMSO ≥ 50 mg/mL
Water 20 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Robert G, et al. PLoS One. Acadesine kills chronic myelogenous leukemia (CML) cells through PKC-dependent induction of autophagic cell death.

[2] Nakamaru K, et al. Biochem Biophys Res Commun. AICAR, an activator of AMP-activated protein kinase, down-regulates the insulin receptor expression in HepG2 cells.

[3] Campas C, et al. Blood. Acadesine activates AMPK and induces apoptosis in B-cell chronic lymphocytic leukemia cells but not in T lymphocytes.

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Keywords: AICAR, Acadesine; AICA Riboside supplier, AMPK, inhibitors, activators

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