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In vitro: 10-HCPT has an inhibitory effect on phosphorylation of histone H1 and H3 in murine hepatoma cells and also exhibits a differentiation inducing effect in human HepG2 cells. 10-HCPT inhibits the cell growth, reduces the cell viability and disturbs the cell cycle distribution of human colon cancer cell line Colo 205. It induces cell cycle arrest in the G2/M phase.
In vivo: 10-HCPT can be safely orally administered and, alternatively, a low-dose long-term treatment. Prolonged elimination of HCPT in vivo may have a significant impact on its therapeutic effects. HCPT is metabolized to its carboxylate form and glucuronides. Dose-dependent toxicity is observed with i.v. administration of HCPT.
| Cell Experiment | |
|---|---|
| Cell lines | Colo 205 cells |
| Preparation method | Colo 205 cells (5×105) (ATCC:CCL-222) are seeded in 25T flasks overnight and then treated without (control) and with 5, 10, 15 or 20 nM of HCPT, respectively. After treatment for 24-120 h, cells are harvested by trypsin-EDTA and then centrifuged at 1,500 rpm for 5 min at 4˚C. The cell pellet is resuspended in culture medium containing 0.04% trypan blue and the viable cells are enumerated by a hemocytometer. |
| Concentrations | 5, 10, 15 or 20 nM |
| Incubation time | 24-120 h |
| Animal Experiment | |
|---|---|
| Animal models | BALB/c-nude mice |
| Formulation | propylene glycol |
| Dosages | 1, 2.5, 5, 7.5 mg/kg |
| Administration | oral administration |
| Molecular Weight | 364.35 |
| Formula | C20H16N2O5 |
| CAS Number | 64439-81-2 |
| Solubility (25°C) | 29 mg/mL in DMSO |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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