Z-YVAD-FMK

Biological Activity

Z-YVAD-FMK is a synthetic peptide that irreversibly inhibits activity of caspase-1. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies.

Product Citations

• 

  Environ Pollut. 2020 Oct 23;273:115919.

  Inhibition of ROS/NLRP3/Caspase-1 mediated pyroptosis attenuates cadmium-induced apoptosis in duck renal tubular epithelial cells
  Z-YVAD-FMK purchased from AbMole

• 

  2021 May 25 .

  Caspase-1 Inhibitor Reduces Pyroptosis in Kidney Injury Induced by Brain Death
  Z-YVAD-FMK purchased from AbMole

• 

  J Hazard Mater. 2021 Aug 15;416:126138.

  New insights into crosstalk between pyroptosis and autophagy co-induced by molybdenum and cadmium in duck renal tubular epithelial cells
  Z-YVAD-FMK purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Cell Physiol Biochem (2018). Figure 3. Z-VAD-FMK
	Method	western blotting
	Cell Lines	GBM cells
	Concentrations	20 μM
	Incubation Time	3 h
	Results	To examine whether RA-induced apoptosis was caspase-dependent, we introduced the pan-caspase inhibitor z-VAD-fmk. As shown in Fig. 3D, combined treatment with z-VAD-fmk rescued RA-induced cell death.

Chemical Information

Molecular Weight	630.66
Formula	C31H39FN4O9
CAS Number	210344-97-1
Solubility (25°C)	DMSO: ≥ 25 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Gicquel T, et al. FASEB J. IL-1β production is dependent on the activation of purinergic receptors and NLRP3 pathway in human macrophages.

[2] Franklin BS, et al. Nat Immunol. The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation.