Z-YVAD-FMK

Biological Activity

Z-YVAD-FMK is a synthetic peptide that irreversibly inhibits activity of caspase-1. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies.

Product Citations

• 

  2021 May 25 .

  Caspase-1 Inhibitor Reduces Pyroptosis in Kidney Injury Induced by Brain Death
  Z-YVAD-FMK purchased from AbMole

• 

  J Hazard Mater. 2021 Aug 15;416:126138.

  New insights into crosstalk between pyroptosis and autophagy co-induced by molybdenum and cadmium in duck renal tubular epithelial cells
  Z-YVAD-FMK purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Cell Physiol Biochem (2018). Figure 3. Z-VAD-FMK
	Method	western blotting
	Cell Lines	GBM cells
	Concentrations	20 μM
	Incubation Time	3 h
	Results	To examine whether RA-induced apoptosis was caspase-dependent, we introduced the pan-caspase inhibitor z-VAD-fmk. As shown in Fig. 3D, combined treatment with z-VAD-fmk rescued RA-induced cell death.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information

References

IL-1β production is dependent on the activation of purinergic receptors and NLRP3 pathway in human macrophages.
Gicquel T, et al. FASEB J. 2015 Oct;29(10):4162-73. PMID: 26116704.

The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation.
Franklin BS, et al. Nat Immunol. 2014 Aug;15(8):727-37. PMID: 24952505.