Solanesol mainly accumulates in solanaceous crops, including tobacco, tomato, potato, eggplant, and pepper plants. Solanesol binds at ATP binding site and inhibit the phosphorylation of Focal Adhesion Kinase (FAK). Solanesol upregulates HO-1 and Hsp70 expression in L02 cells which activate Nrf2 and HSF1. It induces Nrf2 by enhancement of Keap1 interaction with Hsp90. Solanesol-activated HSF1 is involved in chaperones interaction.
| Cell Experiment | |
|---|---|
| Cell lines | human hepatic cell L02 |
| Preparation method | Cell viability is determined by MTT assays. Cells are seeded into 96-well plates (5 × 104 cells/well) 24 h before treatment. The cells are then treated with various concentrations of solanesol followed by incubating with 5 mg/ml of MTT working solution for 4 h at 37 °C. After being treated with 100 μL of DMSO to dissolve the crystals, the cells are detected under an Microplate Reader to measure the absorbance at 570 nm. |
| Concentrations | 10, 50, 100, 250, 500, 1000 μM |
| Incubation time | 12 h |
| Animal Experiment | |
|---|---|
| Animal models | Male albino rats |
| Formulation | Gelling agent Carbopol-934 |
| Dosages | 0.2 g of gel containing solanesol (1 % w/v) |
| Administration | applied to the plantar surface of the right hind paw by gently rubbing with the index finger |
| Molecular Weight | 631.07 |
| Formula | C45H74O |
| CAS Number | 13190-97-1 |
| Solubility (25°C) | Soluble in Chloroform |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related FAK Products |
|---|
| Petunidin chloride
Petunidin chloride is an O-methylated anthocyanidin derived from delphinidin. *The compound is unstable in solutions, freshly prepared is recommended. |
| FAK-IN-11
FAK-IN-11 is a FAK inhibitor. |
| ZINC40099027
ZINC40099027 is a FAK activator. |
| FC-11
FC-11 is a PROTAC FAK degrader (DC90: 1 nM) . |
| FAK-IN-9
FAK-IN-9 is a potent and orally active FAK inhibitor with an IC50 of 27.44 nM. |
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