Free shipping on all orders over $ 500

PF-562271

Cat. No. M2302
PF-562271 Structure
Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mM*1mL in DMSO USD 126  USD140 In stock
2mg USD 72  USD80 In stock
5mg USD 108  USD120 In stock
10mg USD 162  USD180 In stock
50mg USD 396  USD440 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
Biological Activity

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK and Pyk2 with IC50 of 1.5 nM and 14 nM, respectively. Treatment with PF-562271 impaires cell viability in all cell lines, with an average IC50 of 2.4 μM after 3 days of treatment. TC32 and A673 are the 2 most sensitive cell lines, with IC50 concentrations of 2.1 and 1.7 μM, respectively. 

In vivo, Rats received PF-562271 demonstrate a decrease in tumor growth after 2 weeks of treatment with signs of bone healing as evidenced by the deposition of new bone (cortical and cancellous) at sites previously damaged by the tumor.

Product Citations
Customer Product Validations & Biological Datas
Source AM J Resp Crit Care (2018). Figure E2. PF562271 (Abmole Bioscience)
Method qPCR
Cell Lines Lung fibroblasts
Concentrations 10 μM
Incubation Time 24 h
Results Focal Adhesion Kinase (PF562271), ALK5 (A83-01), or MTRF (CCG-100602) did not consistently restore fibroblast miR-29 levels on IPF-ECM
Source Mol Cancer Ther. (2015) Figure 5. PF-562271 (M2302, Abmole, shanghai, China)
Method western blotting, immunohistochemical staining(IHC)
Cell Lines SKOV3 cells
Concentrations
Incubation Time
Results NISCH suppressed tumor metastasis via inhibiting FAK signal transduction in vivo.
Source Mol Cancer Ther. (2015) Figure 5. PF-562271 (M2302, Abmole, shanghai, China)
Method western blotting, immunohistochemical staining(IHC)
Cell Lines SKOV3 cells
Concentrations
Incubation Time 72h
Results Nischarin attenuated cell invasion by inhibiting FAK signal transduction.
Protocol
Cell Experiment
Cell lines MPanc-96 or MAD08-608 cells
Preparation method Cell migration and invasion assays were performed using transwell migration chambers (BD Biosciences, Bedford, MA) as previously described (26–28). Conditioned media was prepared from subconfluent MPanc-96 or MAD08-608 cells by harvesting growth medium (containing 0.5% FBS) for two 24 h periods. The conditioned media was centrifuged to remove cellular debris and concentrated 3 fold using Amicon Ultra-15 3K centrifugal filter devices (Millipore, Billerica, MA). Mock conditioned media was prepared by concentrating 0.5% FBS in similar fashion. For proliferation assays, cells (1.5 × 105) were plated in 96-well plates in 1% FBS-containing media and cell number was measured using the CyQuant assay (Invitrogen, Grand Island, NY).
Concentrations 0~1µM
Incubation time 48h
Animal Experiment
Animal models Orthotopic tumor model (Six-week old male athymic nude mice)
Formulation saline
Dosages 33 mg/kg twice/day
Administration by gastric lavage
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 507.49
Formula C21H20F3N7O3S
CAS Number 717907-75-0
Purity >99%
Solubility DMSO ≥ 60 mg/mL
Storage at -20°C
Related FAK Products
BI-4464

BI-4464 is a novel highly selective ATP competitive inhibitor of PTK2/FAK with IC50 of 17 nM, it is also a PTK2 ligand for PROTAC.

Solanesol

Solanesol (Nonaisoprenol) is a long-chain polyisoprenoid alcohol compound with inhibitory activities toward FAK.

Defactinib hydrochloride

Defactinib hydrochloride is an orally bioavailable, small-molecule focal adhesion kinase (FAK) inhibitor with potential antiangiogenic and antineoplastic activities.

GSK2256098

GSK2256098 is a potent, selective, reversible, and ATP competitive FAK kinase inhibitor with apparent Ki of 0.4 nM.

Y15

Y15 is a novel small molecule FAK phosphorylation inhibitor; specifically block phosphorylation of Y397-FAK and total phosphorylation of FAK.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: PF-562271 supplier, FAK, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.