Protein Synthesis IN-1

Biological Activity

Protein Synthesis IN-1 (a substitute for Cycloheximide) is an inhibitor of protein biosynthesis in eukaryotic organisms, with IC50 of 532.5 nM. Protein Synthesis IN-1 binds to the E-site of the 60S subunit of the ribosome during the protein synthesis process in eukaryotic cells, blocking the ribosome translocation process mediated by eEF2 and preventing the synthesis of new proteins. Protein Synthesis IN-1 suppresses ferroptosis and inhibits autophagy. Protein Synthesis IN-1 exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome) thus blocking translational elongation. Protein Synthesis IN-1 is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.

Product Citations

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  Mol Cell. 2025 Jun 19;85(12):2320-2336.e9.

  The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance
  Protein Synthesis IN-1 purchased from AbMole

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  Nat Commun. 2025 Jul 01;16(1):5496.

  Mammalian tRNA acetylation determines translation efficiency and tRNA quality control
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Rep. 2025 Feb 19; .

  HSPA8 dampens SCAP/INSIG split and SREBP activation by reducing PKR-mediated INSIG phosphorylation
  Protein Synthesis IN-1 purchased from AbMole

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  Int Immunopharmacol. 2025 Jan 10;144:113564.

  ITGB4/GNB5 axis promotes M2 macrophage reprogramming in NSCLC metastasis
  Protein Synthesis IN-1 purchased from AbMole

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  J Virol. 2025 Jan 31;99(1):e0127724.

  Swine RNF5 positively regulates the antiviral activity of IFITM1 by mediating the degradation of ABHD16A
  Protein Synthesis IN-1 purchased from AbMole

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  J Pharm Sci. 2025 Jul 08; .

  Impact of droplet size and surface charge on oral bioavailability of Norcantharidin-loaded SEDDS: Mechanistic insights into lymphatic transport and intestinal permeability
  Protein Synthesis IN-1 purchased from AbMole

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  Mol Biol Rep. 2025 Jun 24;52(1):633.

  UBE3C promotes pancreatic ductal adenocarcinoma progression by catalysing p53 ubiquitination
  Protein Synthesis IN-1 purchased from AbMole

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  Mol Cancer. 2024 Dec 19;23(1):274.

  ZDHHC20 mediated S-palmitoylation of fatty acid synthase (FASN) promotes hepatocarcinogenesis
  Protein Synthesis IN-1 purchased from AbMole

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  Adv Sci (Weinh). 2024 Jan;e2207435.

  Defective Homologous Recombination Repair By Up-Regulating Lnc-HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage
  Protein Synthesis IN-1 purchased from AbMole

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  Autophagy. 2024 Jun 6.

  The novel lnc-HZ12 suppresses autophagy degradation of BBC3 by preventing its interactions with HSPA8 to induce trophoblast cell apoptosis
  Protein Synthesis IN-1 purchased from AbMole

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  Brain. 2024 Jun 25.

  Heterozygous variants in USP25 cause genetic generalized epilepsy
  Protein Synthesis IN-1 purchased from AbMole

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  Environ Int. 2024 Sep;191:108975.

  BaP/BPDE suppresses homologous recombination repair in human trophoblast cells to induce miscarriage: The roles of lnc-HZ08
  Protein Synthesis IN-1 purchased from AbMole

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  Cancer Lett. 2024 Apr 6;590:216861.

  TRAF6 enhances PD-L1 expression through YAP1-TFCP2 signaling in melanoma
  Protein Synthesis IN-1 purchased from AbMole

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  Free Radic Biol Med. 2024 Aug 23;224:130-143.

  Prohibitin 2 confers NADPH oxidase 1-mediated cytosolic oxidative signaling to promote gastric cancer progression by ERK activation
  Protein Synthesis IN-1 purchased from AbMole

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  iScience. 2024 May 15.

  Deficiency in Prader-Willi syndrome gene necdin leads to attenuated cardiac contractility
  Protein Synthesis IN-1 purchased from AbMole

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  PLoS Pathog. 2024 Sep 30;20(9):e1012594.

  TRIM56 restricts Coxsackievirus B infection by mediating the ubiquitination of viral RNA-dependent RNA polymerase 3D
  Protein Synthesis IN-1 purchased from AbMole

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  J Biol Chem. 2024 Mar 25;300(5):107226.

  Epstein-Barr virus suppresses N6-Methyladenosine modification of TLR9 to promote immune evasion
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Signal. 2024 May 26;111235.

  BMSC derived EVs inhibit colorectal Cancer progression by transporting MAGI2-AS3 or something similar
  Protein Synthesis IN-1 purchased from AbMole

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  Respir Res. 2024 Jul 25;25(1):287.

  Novel hypoxia-induced HIF-1αactivation in asthma pathogenesis
  Protein Synthesis IN-1 purchased from AbMole

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  Mol Carcinog. 2024 Jan.

  SN-38, an active metabolite of irinotecan, inhibits transcription of nuclear factor erythroid 2-related factor 2 and enhances drug sensitivity of colorectal cancer cells
  Protein Synthesis IN-1 purchased from AbMole

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  iScience. 2024 Jan 19.

  FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-β pathway via facilitating CD109 expression
  Protein Synthesis IN-1 purchased from AbMole

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  Mol Cell Biochem. 2024 Feb 17.

  Tripartite-motif 3 represses ovarian cancer progression by downregulating lactate dehydrogenase A and inhibiting AKT signaling
  Protein Synthesis IN-1 purchased from AbMole

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  Sci Rep. 2024 Oct 10;14(1):23685.

  USP10 promotes cell proliferation, migration, and invasion in NSCLC through deubiquitination and stabilization of EIF4G1
  Protein Synthesis IN-1 purchased from AbMole

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  Viruses. 2024 Sep 23;16(9):1503.

  N-Acetylcysteine Inhibits Coxsackievirus B3 Replication by Downregulating Eukaryotic Translation Elongation Factor 1 Alpha 1
  Protein Synthesis IN-1 purchased from AbMole

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  Leuk Res. 2024 Jun 29;52(1):46.

  Combining the novel FLT3 and MERTK dual inhibitor MRX-2843 with venetoclax results in promising antileukemic activity against FLT3-ITD AML
  Protein Synthesis IN-1 purchased from AbMole

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  Patent. CN117535410A 2024 Feb 09.

  Patent. CN117535410A
  Protein Synthesis IN-1 purchased from AbMole

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  J Transl Med. 2023 May 5;21(1):303.

  SYTL2 promotes metastasis of prostate cancer cells by enhancing FSCN1-mediated pseudopodia formation and invasion
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Death Discov. 2023 Apr 26.

  Ammonium Tetrathiomolybdate Relieves Oxidative Stress in Cisplatin-induced Acute Kidney Injury via NRF2 Signaling Pathway
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Death Discov. 2023 Jul 25;9(1):259.

  Ammonium tetrathiomolybdate relieves oxidative stress in cisplatin-induced acute kidney injury via NRF2 signaling pathway
  Protein Synthesis IN-1 purchased from AbMole

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  J Cell Mol Med. 2023 Aug 29.

  Alleviation of temporomandibular joint osteoarthritis by targeting RIPK1-mediated inflammatory signalling
  Protein Synthesis IN-1 purchased from AbMole

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  FASEB J. 2023 Oct;37(10):e23183.

  IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6A-dependent manner
  Protein Synthesis IN-1 purchased from AbMole

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  New Journal of Chemistry. 2023 Jun 20.

  Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection
  Protein Synthesis IN-1 purchased from AbMole

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  New J. Chem. 2023 Jun 28;47, 14344-14354.

  Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection
  Protein Synthesis IN-1 purchased from AbMole

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  Biochem Genet. 2023 Nov 11.

  Functional Characterization of a Novel SLC4A4 Variant and Uniparental Isodisomy in Proximal Renal Tubular Acidosis Patient
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Death Differ. 2022 Jul;29(7):1395-1408.

  SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK
  Protein Synthesis IN-1 purchased from AbMole

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  Research Square. 2022 Oct 12.

  IGF2BP2 promotes ovarian cancer growth and metastasis via upregulating CKAP2L protein expression in a m6A-dependent manner
  Protein Synthesis IN-1 purchased from AbMole

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  Ecotoxicol Environ Saf. 2022 Jun 1;237:113564.

  Environmental BPDE induced human trophoblast cell apoptosis by up-regulating lnc-HZ01/p53 positive feedback loop
  Protein Synthesis IN-1 purchased from AbMole

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  Virol Sin. 2022 Aug 16;S1995-820X(22)00143-2.

  CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Biol Toxicol. 2022 Apr;38(2):291-310.

  Lnc-HZ08 regulates BPDE-induced trophoblast cell dysfunctions by promoting PI3K ubiquitin degradation and is associated with miscarriage
  Protein Synthesis IN-1 purchased from AbMole

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  Bioengineered. 2022 Apr;13(4):8000-8012.

  UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization
  Protein Synthesis IN-1 purchased from AbMole

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  J Biol Chem. 2022 Nov 21;102734.

  Ubiquitin-specific peptidase 14 maintains estrogen receptor α stability via its deubiquitination activity in endometrial cancer
  Protein Synthesis IN-1 purchased from AbMole

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  Mol Cell Endocrinol. 2022 Apr 5;545:111575.

  An intron mutation of HNF1A causes abnormal splicing and impairs its activity as a transcription factor
  Protein Synthesis IN-1 purchased from AbMole

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  Adv Sci (Weinh). 2021 Feb.

  Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple‐Negative Breast Cancer Tumor Growth
  Protein Synthesis IN-1 purchased from AbMole

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  Adv Sci (Weinh). 2021 Jan 6;8(4):2003205.

  Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple-Negative Breast Cancer Tumor Growth
  Protein Synthesis IN-1 purchased from AbMole

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  Clin Transl Med. 2021 Apr;11(4):e395.

  FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression
  Protein Synthesis IN-1 purchased from AbMole

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  Int J Mol Sci. 2021 Aug 16;22(16):8779.

  Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency
  Protein Synthesis IN-1 purchased from AbMole

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  J Cancer. 2021 Oct 17;12(23):7041-7051.

  N6-methyladenosine regulates ATM expression and downstream signaling
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Rep. 2020 Jul 28;32(4):107974.

  L ARP7 Is a BRCA1 Ubiquitinase Substrate and Regulates Genome Stability and Tumorigenesis
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Rep. 2020 Jul 28;32(4):107974.

  LARP7 is a BRCA1 ubiquitinase substrate and regulates genome stability and tumorigenesis
  Protein Synthesis IN-1 purchased from AbMole

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  Theranostics. 2020 Apr 27;10(13):5671-5686.

  SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Oncol (Dordr). 2020 Oct 19.

  Ubiquitin-specific protease 2a promotes hepatocellular carcinoma progression via deubiquitination and stabilization of RAB1A
  Protein Synthesis IN-1 purchased from AbMole

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  Cell Microbiol. 2020 Mar;22(3):e13148.

  Hepatitis B virus X protein modulates upregulation of DHX9 to promote viral DNA replication.
  Protein Synthesis IN-1 purchased from AbMole

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  Life Sci. 2020 Sep 15;257:118021.

  TRIB3 destabilizes tumor suppressor PPARα expression through ubiquitin-mediated proteasome degradation in acute myeloid leukemia
  Protein Synthesis IN-1 purchased from AbMole

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  Front Cell Infect Microbiol. 2020 Apr 16;10:158.

  Difluoromethylornithine, a Decarboxylase 1 Inhibitor, Suppresses Hepatitis B Virus Replication by Reducing HBc Protein Levels
  Protein Synthesis IN-1 purchased from AbMole

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  Aging (Albany NY). 2018 Oct 18;10(10):2783-2799.

  Ubiquitin-specific protease 4 promotes metastasis of hepatocellular carcinoma by increasing TGF-β signaling-induced epithelial-mesenchymal transition.
  Protein Synthesis IN-1 purchased from AbMole

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  Biotechnol Lett. 2018 Aug;40:1181-1188.

  UHRF2 promotes DNA damage response by decreasing p21 via RING finger domain.
  Protein Synthesis IN-1 purchased from AbMole

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  Protein Cell. 2017 Mar;8(3):202-218.

  E3 ligase UHRF2 stabilizes the acetyltransferase TIP60 and regulates H3K9ac and H3K14ac via RING finger domain
  Protein Synthesis IN-1 purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Biotechnol Lett (2018 Aug). Figure 3. cycloheximide (Abmole Bioscience)
	Method	Western blotting
	Cell Lines	Human embryonic kidney 293 cells
	Concentrations	50 ug/mL
	Incubation Time	3 h
	Results	The overexpression of UHRF2 shortened the half-life of p21 to 1 h compared to 3 h under controlled conditions.

	Source	Protein and Cell (2016). Figure 4.CHX (ABmole Bioscience, USA)
	Method	Half-life experiments
	Cell Lines	Human normal liver cells (LO2)
	Concentrations	100 μg/mL
	Incubation Time
	Results	Graph represents collated results rom three independent half-life experiments performed in HEK293, LO2 and HepG2 cells. TIP60 levels were normalized to 1 at time zero.

Chemical Information

Molecular Weight	281.4
Solubility (25°C)	DMSO ≥ 60 mg/mL Water 10 mg/mL (ultrasonic and warming)
Storage	4°C, protect from light