All AbMole products are for research use only, cannot be used for human consumption.
Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome (λex=495 nm, λem=520 nm). Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength.
| Molecular Weight | 788.87 |
| Formula | C35H48N8O11S |
| CAS Number | 17466-45-4 |
| Solubility (25°C) | Water |
| Storage | -20°C, protect from light, dry, sealed |
[4] H Faulstich, et al. Adv Exp Med Biol. New aspects of amanitin and phalloidin poisoning
[5] J A Cooper. J Cell Biol. Effects of cytochalasin and phalloidin on actin
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