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Phalloidin

Cat. No. M11153

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Phalloidin Structure
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1mg USD 440  USD440 In stock
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Biological Activity

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome (λex=495 nm, λem=520 nm). Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength.

Chemical Information
Molecular Weight 788.87
Formula C35H48N8O11S
CAS Number 17466-45-4
Solubility (25°C) Water
Storage -20°C, protect from light, dry, sealed
References

[1] Hanieh Mazloom-Farsibaf, et al. PLoS One. Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells

[2] Sanchaita Das, et al. Structure. D-loop Dynamics and Near-Atomic-Resolution Cryo-EM Structure of Phalloidin-Bound F-Actin

[3] Sabrina Pospich, et al. Structure. Structural Effects and Functional Implications of Phalloidin and Jasplakinolide Binding to Actin Filaments

[4] H Faulstich, et al. Adv Exp Med Biol. New aspects of amanitin and phalloidin poisoning

[5] J A Cooper. J Cell Biol. Effects of cytochalasin and phalloidin on actin

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