Free shipping on all orders over $ 500

Fluo-4 AM

Cat. No. M3836
Fluo-4 AM Structure
Size Price Availability Quantity
100ug USD 150  USD150 In stock
1mg USD 785  USD785 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright.

Chemical Information
Molecular Weight 1096.94
Formula C51H50F2N2O23
CAS Number 273221-67-3
Solubility (25°C) DMSO
Storage -20°C, dry, protect from light, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Lina Qiu, et al. Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp

[2] Andrs F Miln, et al. Calibration of mammalian skeletal muscle Ca 2+ transients recorded with the fast Ca 2+ dye Mag-Fluo-4

[3] Ana M Rossi, et al. Reliable measurement of free Ca 2+ concentrations in the ER lumen using Mag-Fluo-4

[4] Kateri J Spinelli, et al. Monitoring intracellular calcium ion dynamics in hair cell populations with Fluo-4 AM

[5] Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM

Related Fluorescent Dye Products

Zinquin is a zinc-sensitive, cell-impermeable fluorescent probe. Excitation (nm) 355, Emission (nm) 491

IRDye 800 NHS

IRDye 800 NHS is an active ester that can be used to label biomolecules, such as proteins and nucleic acids, in order to track and detect the dynamic behavior of these molecules in molecular biology research. IRDye 800CW NHS has good water solubility and can be bound to biomolecules, and the biomolecules still maintain their original biological activity after binding.


SOSG (Singlet Oxygen Sensor Green Fluorescent Probe) is highly selective for singlet oxygen (1O2). It is commonly used for the detection of singlet oxygen in solutions and the evaluation of the efficacy of free radical scavengers.


FM1-43 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers.


BCIP is a chromogenic substrate for colorimetric assay of alkaline phosphatase activity.

Abmole Inhibitor Catalog

Keywords: Fluo-4 AM supplier, Fluorescent Dye, inhibitors, activators

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.