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Fluo-4 AM

Cat. No. M3836
Fluo-4 AM Structure
Size Price Availability Quantity
100ug USD 150  USD150 In stock
1mg USD 785  USD785 In stock
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Quality Control
  • Current batch:
  • Purity >95%
  • COA
  • MSDS
Biological Activity

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 1096.94
Formula C51H50F2N2O23
CAS Number 273221-67-3
Purity >95%
Solubility DMSO
Storage -20°C, dry, protect from light, sealed
References

[1] Lina Qiu, et al. Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp

[2] Andrs F Miln, et al. Calibration of mammalian skeletal muscle Ca 2+ transients recorded with the fast Ca 2+ dye Mag-Fluo-4

[3] Ana M Rossi, et al. Reliable measurement of free Ca 2+ concentrations in the ER lumen using Mag-Fluo-4

[4] Kateri J Spinelli, et al. Monitoring intracellular calcium ion dynamics in hair cell populations with Fluo-4 AM

[5] Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM

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