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Fluo-4 AM

Cat. No. M3836
Fluo-4 AM Structure
Size Price Availability Quantity
100ug USD 150  USD150 In stock
1mg USD 785  USD785 In stock
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Quality Control & Documentation
Biological Activity

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright.

Chemical Information
Molecular Weight 1096.94
Formula C51H50F2N2O23
CAS Number 273221-67-3
Solubility (25°C) DMSO
Storage -20°C, dry, protect from light, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Lina Qiu, et al. Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp

[2] Andrs F Miln, et al. Calibration of mammalian skeletal muscle Ca 2+ transients recorded with the fast Ca 2+ dye Mag-Fluo-4

[3] Ana M Rossi, et al. Reliable measurement of free Ca 2+ concentrations in the ER lumen using Mag-Fluo-4

[4] Kateri J Spinelli, et al. Monitoring intracellular calcium ion dynamics in hair cell populations with Fluo-4 AM

[5] Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM

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