Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright.
|Storage||-20°C, dry, protect from light, sealed|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
 Lina Qiu, et al. Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
 Andrs F Miln, et al. Calibration of mammalian skeletal muscle Ca 2+ transients recorded with the fast Ca 2+ dye Mag-Fluo-4
 Ana M Rossi, et al. Reliable measurement of free Ca 2+ concentrations in the ER lumen using Mag-Fluo-4
 Kateri J Spinelli, et al. Monitoring intracellular calcium ion dynamics in hair cell populations with Fluo-4 AM
 Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM
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Mito Red is a cell membrane permeable rhodamine based dye. It localizes mitochondria, and emits red fluorescence. λex 569 nm; λem 594 nm in DMSO
6-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase.
5-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase.
5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.
5-FAM SE is a green fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates.
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