In vitro: G5 is capable of activating an apoptosome-independent apoptotic pathway. It targets the ubiquitinproteasome system by inhibiting the ubiquitin isopeptidases. G5 induces a rather unique apoptotic pathway, which includes a Bcl-2-dependent but apoptosome-independent mitochondrial pathway with upregulation of the BH3-only protein Noxa, stabilization of the inhibitor of apoptosis antagonist Smac, but also the involvement of the death receptor pathway. G5 is a potent apoptotic inducer showing IC50s of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively.*The compound is unstable in solutions, freshly prepared is recommended
| Cell Experiment | |
|---|---|
| Cell lines | IMR90-E1A (E1A) and E1A/C9DN cells |
| Preparation method | Western blot of E1A cell lysates treated with stealth RNAi oligos specific for Apaf-1 or HDAC4 as indicated. E1A cells transfected with Apaf-1 or HDAC4-specific RNAi oligos were incubated for 20 hours with the indicated apoptotic insults. |
| Concentrations | 1.25 μmol/L |
| Incubation time | 20 h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 414.39 |
| Formula | C19H14N2O7S |
| CAS Number | 108477-18-5 |
| Form | Solid |
| Solubility (25°C) | 10mM in DMSO |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related Apoptosis Products |
|---|
| Cytochrome C (from Pig Heart)
Cytochrome C (from Pig Heart) is a multifunctional enzyme involved in cellular life and death decisions.Cytochrome C is critical in mitochondrial electron transport and intrinsic type II apoptosis. |
| Celosin K
Celosin K, a triterpenoid saponin isolated from the seeds of Semen Celosiae, is also a potent inhibitor of t-BHP-induced neuronal damage. In addition, Celosin K inhibits oxidative stress, apoptosis, and autophagy. |
| GLP-2(rat)
GLP-2(rat) is an intestinal growth factor. |
| Edratide
Edratide (TV 4710) is a synthetic peptide of 19 amino acid based on the complementarity-determining region 1 (CDR1) of a human anti-DNA antibody that expresses a major idiotype denoted 16/6 Id. |
| Thrombospondin-1 (1016-1023) (human, bovine, mouse)
Thrombospondin-1 (1016-1023) (human, bovine, mouse), is the C-terminal end of the native sequence of Thrombospondin-1 (TSP-1), is a CD47 agonist peptide. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.
