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MQAE is a novel chloride ion fluorescence probe excited by ultraviolet light. MQAE, binding bromine ion as ligand, can be used to detect the activity of chloride. MQAE exhibits a reduction of fluorescence intensity with the increase of intracellular Cl- concentration. MQAE has a pH-independent fluorescence intensity basically at a Cl- concentration <50 nM. MQAE has high water solubility and cell permeability, MQAE has been used for fluorescence detection such as confocal microscopy and flow cytometry. MQAE is a fluorescent indicator that is quenched via collision with chloride, and is more sensitive and selective than 36Cl and microelectrode-based methods for chloride measurement in cells. Ex/Em=350/460 nm
| Molecular Weight | 326.19 |
| Formula | C14H16BrNO3 |
| CAS Number | 162558-52-3 |
| Solubility (25°C) | Water 90 mg/mL DMSO ≥ 35 mg/mL |
| Storage | 2-8°C, protect from light, dry, sealed |
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