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Imidazole Ketone Erastin (IKE) is an inducer for ferroptosis iron death. It is also a potent, selective, and metabolically stable system XC -- inhibitor. IKE is more water-soluble than Erastin. Inducing iron death in diffuse large B-cell lymphoma cells (e.g. Sudhl-5/6/16).
| Cell Experiment | |
|---|---|
| Cell lines | DLBCL cell line |
| Preparation method | DLBCL cells are plated at 10,000 cells per well in white 384-well plates (32 μL per well) in technical duplicates and incubated overnight. The cells are then treated with 8 μL medium containing a two-fold dilution series of vehicle (DMSO), IKE (starting from 100 μM) with or without Fer-1 (starting from 200 μM). After 24 h incubation, 40 μL of 50% CellTiter-Glo / 50% cell culture medium is added to each well and incubated at room temperature with shaking for 15 min. Luminescence is measured. All cell viability data are normalized to the DMSO vehicle condition. Experiments are performed three independent times with different passages for each cell line. From these data, dose-response curves and IC50 values are computed. |
| Concentrations | 0-100 μM |
| Incubation time | 24 h |
| Animal Experiment | |
|---|---|
| Animal models | Non-obese diabetic/ severe combined immunodeficiency (NOD/SCID) mice |
| Formulation | dissolved in 5% DMSO/95% HBSS at pH 4 solution |
| Dosages | 50 mg/kg, 17 mg/kg |
| Administration | IP and PO and IV |
| Molecular Weight | 655.14 |
| Formula | C35H35ClN6O5 |
| CAS Number | 1801530-11-9 |
| Solubility (25°C) | DMSO 90 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Related Ferroptosis Products |
|---|
| HBED
HBED is a potent iron chelator. |
| viFSP1
viFSP1 is a species-independent inhibitor of FSP1 that induces ferroptosis in FSP1-dependent cells. |
| icFSP1
icFSP1 is a potent ferroptosis suppressor protein-1 (FSP1) inhibitor. |
| (R)-HTS-3
(R)-HTS-3 is an lysophosphatidylcholine acyltransferase 3 (LPCAT3) inhibitor, with an IC50 of 0.09 μM. |
| CuATSP
CuATSP is a potent inhibitor of ferroptosis. |
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