All AbMole products are for research use only, cannot be used for human consumption.
For this product's availability, delivery time and price, please email [email protected] directly or click the "Inquiry Now" button below.
Hoechst 33258 analog 6 is a anglog of Hoechst stains(Hoechst 33258), which are part of a family of blue fluorescent dyes used to stain DNA. IC50 Value: Target: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.
| Molecular Weight | 536.71 |
| CAS Number | 129244-66-2 |
| Solubility (25°C) | DMSO 25 mg/mL |
| Storage | 2-8°C, protect from light |
| Related Fluorescent Dye Products |
|---|
| DSPE-Rhodamine
DSPE-Rhodamine is formed by the conjugation of DSPE with the Rhodamine fluorescent dye. DSPE possesses a hydrophobic lipid tail and a hydrophilic head group, while Rhodamine is a red fluorescent dye. DSPE-Rhodamine retains the lipid properties of DSPE while also imparting fluorescent labelling capabilities. As a key component of delivery systems, DSPE-Rhodamine enhances targeting and bioavailability. Its lipid properties facilitate the penetration of molecules through cell membranes, while its fluorescent properties enable tracking of distribution and dynamic changes within the body. |
| LD540
LD540 is a novel high-sensitivity lipophilic dye modified with BODIPY fluorescent groups, designed for precise labelling and imaging of lipid droplets. LD540 exhibits excellent photostability and an optimal fluorescence spectrum, making it compatible with various commonly used fluorescent dyes (such as DAPI and Alexa Fluor 647), thereby supporting multi-colour imaging requirements. Additionally, LD540 is suitable for both fixed and live cells and can label ultra-small lipid droplets. |
| PDMPO
PDMPO (Yellow/Blue DND-160) is a ratiometric probe for the determination of lysosomal pH for fluorescence imaging. PDMPO exhibits pH-dependent dual excitation and dual emission peaks. PDMPO produces blue fluorescence (Ex/Em=329 nm/440 nm) in weakly acidic organelles, and in more acidic lysosomes it becomes yellow fluorescence (Ex/Em=384 nm/540 nm). |
| Copper probe CF4
Copper probe CF4 (Copper fluor CF4) is a Cu+-specific fluorescent probe based on a rhodol dye scaffold. Copper probe CF4 (Copper fluor CF4) has high copper selectivity with a Kd value of 2.9×10-13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper probe CF4 (Copper fluor CF4) is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission). Copper probe CF4 (Copper fluor CF4) can be used to study colon cancer. |
| Phalloidin-Fluor 647 Conjugate
Phalloidin-Fluor 647 Conjugate selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
